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  1. 30601
    “…The ability to predict immunogenic regions in selected proteins by in-silico methods has broad implications, such as allowing a quick selection of potential reagents to be used as diagnostics, vaccines, immunotherapeutics, or research tools in several branches of biological and biotechnological research. However, the prediction of antibody target sites in proteins using computational methodologies has proven to be a highly challenging task, which is likely due to the somewhat elusive nature of B-cell epitopes. …”
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    B-Pred, a structure based B-cell epitopes prediction server
  2. 30602
    por O’Grady, Eoin P., Sokol, Pamela A.
    Publicado 2011
    “…Members of the Burkholderia cepacia complex (Bcc) are important in medical, biotechnological, and agricultural disciplines. These bacteria naturally occur in soil and water environments and have adapted to survive in association with plants and animals including humans. …”
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    Burkholderia cenocepacia Differential Gene Expression during Host–Pathogen Interactions and Adaptation to the Host Environment
  3. 30603
    “…Thus, our sequencing and annotation strategies provide a general view of the cellular and molecular processes that take place in these arthropods, allowed the discovery of new pharmacological and biotechnological targets and uncovered several regulatory and metabolic responses behind the assembly of the scorpion venom. …”
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    Global Transcriptome Analysis of the Scorpion Centruroides noxius: New Toxin Families and Evolutionary Insights from an Ancestral Scorpion Species
  4. 30604
    por Ludwig, Marcus, Bryant, Donald A.
    Publicado 2012
    “…It is a model organism for studies of cyanobacterial metabolism and has great potential for biotechnological applications. It exhibits an exceptional tolerance of high-light irradiation and shows very rapid growth. …”
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    Synechococcus sp. Strain PCC 7002 Transcriptome: Acclimation to Temperature, Salinity, Oxidative Stress, and Mixotrophic Growth Conditions
  5. 30605
    “…Furthermore, this seaweed expressed sequences related to terpene biosynthesis, including the complete mevalonate-independent pathway, which offers new possibilities for biotechnological applications using secondary metabolites from L. dendroidea.…”
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    Transcriptomic analysis of the red seaweed Laurencia dendroidea (Florideophyceae, Rhodophyta) and its microbiome
  6. 30606
    “…Optimization of the SSELP process at different levels should further improve the efficiency of microbial lipid technology, which in turn, promote the biotechnological production of fatty acid-derived products from lignocellulosic biomass.…”
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    Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process
  7. 30607
    “…The biodegradation of lignin, one of the most abundant carbon compounds on Earth, has important biotechnological applications in the derivation of useful products from lignocellulosic wastes. …”
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    The CouPSTU and TarPQM Transporters in Rhodopseudomonas palustris: Redundant, Promiscuous Uptake Systems for Lignin-Derived Aromatic Substrates
  8. 30608
    “…The recombinant enzyme (90 kDa) was purified and characterized in order to evaluate its biotechnological potential. Recombinant P. pastoris efficiently secreted β-glucosidase into the medium and produced high level of enzymatic activity (41 U/ml) after 192 h of growth, under methanol induction. …”
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    Cloning, expression and characterization of an ethanol tolerant GH3 β-glucosidase from Myceliophthora thermophila
  9. 30609
    “…Recombinant TaDGAT2 restored TAG biosynthesis to the Saccharomyces cerevisiae H1246 TAG-deficient mutant, and microsomes from the complemented mutant displayed DGAT activity with C16 and C18 saturated and unsaturated fatty acyl-CoA and diacylglycerol substrates. To examine its biotechnological potential, TaDGAT2 was expressed under control of a strong seed-specific promoter in wild-type Arabidopsis thaliana and the high linoleic acid fad3fae1 mutant. …”
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    A thraustochytrid diacylglycerol acyltransferase 2 with broad substrate specificity strongly increases oleic acid content in engineered Arabidopsis thaliana seeds
  10. 30610
    “…Assessments of bacterial community diversity and dynamics are fundamental for the understanding of microbial ecology as well as biotechnological applications. We show that the choice of PCR primers has great impact on the results of analyses of diversity and dynamics using gene libraries and DNA fingerprinting. …”
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    The Choice of PCR Primers Has Great Impact on Assessments of Bacterial Community Diversity and Dynamics in a Wastewater Treatment Plant
  11. 30611
    “…When “wired” with Os(dmbpy)PVI, the graphite electrodes modified with fdgPDH showed a pronounced increase in the current density with J(max) 13- and 6-fold higher than that observed for gPDH- and dgPDH-modified electrodes, making the fragmented enzyme extraordinarily attractive for further biotechnological applications. An easier access of the substrate to the active site and improved communication between the enzyme and mediator matrix are suggested as the two main reasons for the excellent performance of the fdgPDH when compared with that of gPDH and dgPDH. …”
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    Further Insights into the Catalytical Properties of Deglycosylated Pyranose Dehydrogenase from Agaricus meleagris Recombinantly Expressed in Pichia pastoris
  12. 30612
    “…Currently, glycolic acid is produced in a chemical process using fossil resources and toxic chemicals. Biotechnological production of glycolic acid using renewable resources is a desirable alternative. …”
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    Glycolic acid production in the engineered yeasts Saccharomyces cerevisiae and Kluyveromyces lactis
  13. 30613
    “…Importantly, since these pathways are conserved in all eukaryotes, it will be of interest to investigate similar engineering approaches in other cell types of biotechnological interest, such as insect cells and mammalian cells.…”
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    Enhanced membrane protein expression by engineering increased intracellular membrane production
  14. 30614
    “…Several medical and biotechnological applications have emerged utilizing their high collagenolytic efficiency. …”
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    Proteomic protease specificity profiling of clostridial collagenases reveals their intrinsic nature as dedicated degraders of collagen()()
  15. 30615
    “…Hence, the pSiM24 vector would be useful for various plant biotechnological applications. In addition, the pSiM24 plasmid can act as a platform for other applications, such as gene expression studies and different promoter expressional analyses.…”
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    pSiM24 Is a Novel Versatile Gene Expression Vector for Transient Assays As Well As Stable Expression of Foreign Genes in Plants
  16. 30616
    por Pereira-Leal, José B, Abreu, Isabel A, Alabaça, Cláudia S, Almeida, Maria Helena, Almeida, Paulo, Almeida, Tânia, Amorim, Maria Isabel, Araújo, Susana, Azevedo, Herlânder, Badia, Aleix, Batista, Dora, Bohn, Andreas, Capote, Tiago, Carrasquinho, Isabel, Chaves, Inês, Coelho, Ana Cristina, Costa, Maria Manuela Ribeiro, Costa, Rita, Cravador, Alfredo, Egas, Conceição, Faro, Carlos, Fortes, Ana M, Fortunato, Ana S, Gaspar, Maria João, Gonçalves, Sónia, Graça, José, Horta, Marília, Inácio, Vera, Leitão, José M, Lino-Neto, Teresa, Marum, Liliana, Matos, José, Mendonça, Diogo, Miguel, Andreia, Miguel, Célia M, Morais-Cecílio, Leonor, Neves, Isabel, Nóbrega, Filomena, Oliveira, Maria Margarida, Oliveira, Rute, Pais, Maria Salomé, Paiva, Jorge A, Paulo, Octávio S, Pinheiro, Miguel, Raimundo, João AP, Ramalho, José C, Ribeiro, Ana I, Ribeiro, Teresa, Rocheta, Margarida, Rodrigues, Ana Isabel, Rodrigues, José C, Saibo, Nelson JM, Santo, Tatiana E, Santos, Ana Margarida, Sá-Pereira, Paula, Sebastiana, Mónica, Simões, Fernanda, Sobral, Rómulo S, Tavares, Rui, Teixeira, Rita, Varela, Carolina, Veloso, Maria Manuela, Ricardo, Cândido PP
    Publicado 2014
    “…Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. …”
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    A comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencing
  17. 30617
    “…These nanoassemblies display unique properties and can serve as multifunctional platforms for various biotechnological applications.…”
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    Seamless Metallic Coating and Surface Adhesion of Self-Assembled Bioinspired Nanostructures Based on Di-(3,4-dihydroxy-l-phenylalanine) Peptide Motif
  18. 30618
    “…Several Thermus species have shown biotechnological potential such as reduction of heavy metals which is essential for eradication of heavy metal pollution; removing of organic contaminants in water; opening clogged pipes, controlling global warming among many others. …”
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    Analysis of genomic rearrangements, horizontal gene transfer and role of plasmids in the evolution of industrial important Thermus species
  19. 30619
    “…Barnacle adhesion underwater is an important phenomenon to understand for the prevention of biofouling and potential biotechnological innovations, yet so far, identifying what makes barnacle glue proteins ‘sticky’ has proved elusive. …”
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    Adhesive Proteins of Stalked and Acorn Barnacles Display Homology with Low Sequence Similarities
  20. 30620
    “…Horseradish Peroxidase (HRP) is one of the most studied peroxidases and a great number of chemical modifications and genetic manipulations have been carried out on its surface accessible residues to improve its stability and catalytic efficiency necessary for biotechnological applications. Most of the stabilized derivatives of HRP reported to date have involved chemical or genetic modifications of three surface-exposed lysines (K174, K232 and K241). …”
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    How Modification of Accessible Lysines to Phenylalanine Modulates the Structural and Functional Properties of Horseradish Peroxidase: A Simulation Study
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