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  1. 30681
    “…BACKGROUND: The thermotolerant methylotrophic yeast Ogataea polymorpha has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell factory in fermentative productions of chemicals, biofuels and other bio-products. …”
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    Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
  2. 30682
    “…CONCLUSION: Our results provide novel data on the physiological and molecular mechanisms of nervonic acid biosynthesis and oil accumulation in M. oleifera seeds, and will also serve as a starting point for biotechnological genetic engineering for the production of nervonic acid resources. …”
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    Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds
  3. 30683
    “…Currently, several researchers are focused on the development of cell therapies, anti-tumor vaccines, and new biotechnological drugs that have already shown promising results in preclinical studies, therefore, in the near future, we will certainly assist to a new revolution in the field of medical oncology.…”
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    Evolution of Cancer Pharmacological Treatments at the Turn of the Third Millennium
  4. 30684
    “…Halophilic microbes are studied to understand the metabolic pathways adopted by organisms in such extreme environment and for their biotechnological exploitation. In thallosohaline environments worldwide, the autotrophic alga Dunaliella salina Teodoresco is omnipresent, but it is being recently realised that the heterotrophic components vary in different regions. …”
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    The heterotrophic eubacterial and archaeal co-inhabitants of the halophilic Dunaliella salina in solar salterns fed by Bay of Bengal along south eastern coast of India
  5. 30685
    “…Enzymes have a wide variety of applications in diverse biotechnological fields, and the immobilization of enzymes plays a key role in academic research or industrialization due to the stabilization and recyclability it confers. …”
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    Immobilization of Brassica oleracea Chlorophyllase 1 (BoCLH1) and Candida rugosa Lipase (CRL) in Magnetic Alginate Beads: An Enzymatic Evaluation in the Corresponding Proteins
  6. 30686
    “…However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. …”
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    Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance
  7. 30687
    “…The advent of high-throughput biotechnologies, such as mass spectrometry and next-generation sequencing, has led us to further expand dbAMP as a database-assisted platform for providing comprehensively functional and physicochemical analyses for AMPs based on the large-scale transcriptome and proteome data. …”
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    dbAMP: an integrated resource for exploring antimicrobial peptides with functional activities and physicochemical properties on transcriptome and proteome data
  8. 30688
    por Peters, Kristian, Bradbury, James, Bergmann, Sven, Capuccini, Marco, Cascante, Marta, de Atauri, Pedro, Ebbels, Timothy M D, Foguet, Carles, Glen, Robert, Gonzalez-Beltran, Alejandra, Günther, Ulrich L, Handakas, Evangelos, Hankemeier, Thomas, Haug, Kenneth, Herman, Stephanie, Holub, Petr, Izzo, Massimiliano, Jacob, Daniel, Johnson, David, Jourdan, Fabien, Kale, Namrata, Karaman, Ibrahim, Khalili, Bita, Emami Khonsari, Payam, Kultima, Kim, Lampa, Samuel, Larsson, Anders, Ludwig, Christian, Moreno, Pablo, Neumann, Steffen, Novella, Jon Ander, O'Donovan, Claire, Pearce, Jake T M, Peluso, Alina, Piras, Marco Enrico, Pireddu, Luca, Reed, Michelle A C, Rocca-Serra, Philippe, Roger, Pierrick, Rosato, Antonio, Rueedi, Rico, Ruttkies, Christoph, Sadawi, Noureddin, Salek, Reza M, Sansone, Susanna-Assunta, Selivanov, Vitaly, Spjuth, Ola, Schober, Daniel, Thévenot, Etienne A, Tomasoni, Mattia, van Rijswijk, Merlijn, van Vliet, Michael, Viant, Mark R, Weber, Ralf J M, Zanetti, Gianluigi, Steinbeck, Christoph
    Publicado 2018
    “…The research field is dynamic and expanding with applications across biomedical, biotechnological, and many other applied biological domains. …”
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    PhenoMeNal: processing and analysis of metabolomics data in the cloud
  9. 30689
    “…Mucor circinelloides is one of many promising microorganisms; it exhibits diverse biotechnological importance ranging from the production of functional lipids to applications in the manufacture of bio-fuel. …”
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    Engineering of Fatty Acid Synthases (FASs) to Boost the Production of Medium-Chain Fatty Acids (MCFAs) in Mucor circinelloides
  10. 30690
    “…However, the rapidly expanding salinity stress in cultivable areas poses a major peril to crop yield. Among various biotechnological techniques being used to reduce the negative effects of salinity, the use of arbuscular mycorrhizal fungi (AMF) is considered to be an efficient approach for bio-amelioration of salinity stress. …”
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    Mitigation of Salinity Stress in Plants by Arbuscular Mycorrhizal Symbiosis: Current Understanding and New Challenges
  11. 30691
    “…Given the diversity of natural β-(1→3)-glucans and their wide range of biotechnological applications, the identification of enzymatic tools that can act on β-(1→3)-glucooligosaccharides is an attractive area of research. …”
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    Unraveling the subtleties of β-(1→3)-glucan phosphorylase specificity in the GH94, GH149, and GH161 glycoside hydrolase families
  12. 30692
    “…Printed electronics have led to new possibilities in the detection and quantification of a wide range of molecules important for medical, biotechnological, and environmental fields. The integration with microfluidics is often adopted to avoid hand-deposition of little volumes of reagents and samples on miniaturized electrodes that strongly depend on operator’s skills. …”
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    Support-Material-Free Microfluidics on an Electrochemical Sensors Platform by Aerosol Jet Printing
  13. 30693
    “…Mantel and Procrustes correlation analyses with microbial taxonomic data identified not only the well-studied phyla Actinobacteria and Proteobacteria, but also, interestingly, the less biotechnologically exploited phyla Verrucomicrobia and Bacteroidetes, as potential sources harbouring diverse A and KS domains. …”
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    Microbial community drivers of PK/NRP gene diversity in selected global soils
  14. 30694
    “…The prediction of the tridimensional structure of some PGs showed a conserved parallel beta-helical fold related to polysaccharide hydrolysis and a modular architecture, where exons correspond to structural elements. Further biotechnological approaches could target specific softening-related PGs to extend mango post-harvest shelf life.…”
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    Genome-Wide Identification of Mango (Mangifera indica L.) Polygalacturonases: Expression Analysis of Family Members and Total Enzyme Activity During Fruit Ripening
  15. 30695
    “…These findings thus reveal a novel molecular determinant of Si transport in plants and inform breeding, biotechnological, and agricultural practices to effectively utilize Si in the context of plant resilience to environmental stress.…”
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    Si permeability of a deficient Lsi1 aquaporin in tobacco can be enhanced through a conserved residue substitution
  16. 30696
    “…BACKGROUND: In the past two decades, biologists have been able to identify the gene signatures associated with various phenotypes through the monitoring of gene expressions with high-throughput biotechnologies. These gene signatures have in turn been successfully applied to drug development, disease prevention, crop improvement, etc. …”
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    Construction of gene causal regulatory networks using microarray data with the coefficient of intrinsic dependence
  17. 30697
    “…Pichia pastoris is recognized as a biotechnological workhorse for recombinant protein expression. …”
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    Contextualized genome-scale model unveils high-order metabolic effects of the specific growth rate and oxygenation level in recombinant Pichia pastoris
  18. 30698
    “…Thermoalkaliphilic lipases from the I.5 family, with their native advantages such as high thermostability and tolerance to alkaline pHs, are a target for biotechnological applications. Although several strategies have been applied to increase lipases activity, the enhancement through protein engineering without compromising other capabilities is still elusive. …”
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    Disulfide Engineered Lipase to Enhance the Catalytic Activity: A Structure-Based Approach on BTL2
  19. 30699
    por Yang, Xinjia, Peng, Jialin, Pan, Junmin
    Publicado 2019
    “…BACKGROUND: Chlamydomonas reinhardtii is a unicellular green alga, which is a most commonly used model organism for basic research and biotechnological applications. Generation of transgenic strains, which usually requires selectable markers, is instrumental in such studies/applications. …”
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    Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas
  20. 30700
    “…These PpCRN-associated mechanisms are now targets of biotechnological studies aiming to break pathogen’s virulence and to promote plant resistance.…”
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    PpCRN7 and PpCRN20 of Phythophthora parasitica regulate plant cell death leading to enhancement of host susceptibility
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