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31381por Bellora, Nicolás, Moliné, Martín, David-Palma, Márcia, Coelho, Marco A., Hittinger, Chris Todd, Sampaio, José P., Gonçalves, Paula, Libkind, Diego“…Although there are a few edible Tremella spp., the only species with current biotechnological use is the astaxanthin-producing yeast Phaffia rhodozyma (Cystofilobasidiales). …”
Publicado 2016
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31382por Choo, Jin Ho, Hong, Chang Pyo, Lim, Jae Yun, Seo, Jeong-Ah, Kim, Young-Suk, Lee, Dong Wook, Park, Sin-Gi, Lee, Gir Won, Carroll, Emily, Lee, Yin-Won, Kang, Hyun Ah“…BACKGROUND: Genomic studies on fungal species with hydrolytic activity have gained increased attention due to their great biotechnological potential for biomass-based biofuel production. …”
Publicado 2016
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31383por Constable, Ian J., Pierce, Cora M., Lai, Chooi-May, Magno, Aaron L., Degli-Esposti, Mariapia A., French, Martyn A., McAllister, Ian L., Butler, Steve, Barone, Samuel B., Schwartz, Steven D., Blumenkranz, Mark S., Rakoczy, Elizabeth P.“…FUNDING: National Health and Medical Research Council of Australia (AP1010405), Lions Eye Institute, Perth Australia, Avalanche Biotechnologies, Menlo Pk, CA, USA.…”
Publicado 2016
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31384por Hobbs, Christopher K., Porter, Vanessa L., Stow, Maxwell L. S., Siame, Bupe A., Tsang, Herbert H., Leung, Ka Yin“…Therefore, developing efficient methods to identify effectors scattered in bacterial genomes can lead to a better understanding of host-pathogen interactions and ultimately to important medical and biotechnological applications. RESULTS: We used 21 genomic and proteomic attributes to create a precise and reliable T3SS effector prediction method called Genome Search for Effectors Tool (GenSET). …”
Publicado 2016
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31385por Le Quéré, Antoine, Tak, Nisha, Gehlot, Hukam Singh, Lavire, Celine, Meyer, Thibault, Chapulliot, David, Rathi, Sonam, Sakrouhi, Ilham, Rocha, Guadalupe, Rohmer, Marine, Severac, Dany, Filali-Maltouf, Abdelkarim, Munive, Jose-Antonio“…Finally, given the original phylogeographic distribution and the different hosts with which it can develop a beneficial symbiotic interaction, Ensifer aridi may provide new biotechnological opportunities for degraded land restoration initiatives in the future. …”
Publicado 2017
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31386por Zhou, Junpei, Song, Zhifeng, Zhang, Rui, Liu, Rui, Wu, Qian, Li, Junjun, Tang, Xianghua, Xu, Bo, Ding, Junmei, Han, Nanyu, Huang, Zunxi“…These characteristics suggest that the enzyme has versatile potential in biotechnological applications, such as bioconversion of chitin waste and the processing of marine materials and saline foods. …”
Publicado 2017
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31387por Han, Nanyu, Miao, Huabiao, Ding, Junmei, Li, Junjun, Mu, Yuelin, Zhou, Junpei, Huang, Zunxi“…BACKGROUND: Xylanases have been widely employed in many industrial processes, and thermophilic xylanases are in great demand for meeting the high-temperature requirements of biotechnological treatments. In this work, we aim to improve the thermostability of XynCDBFV, a glycoside hydrolase (GH) family 11 xylanase from the ruminal fungus Neocallimastix patriciarum, by site-directed mutagenesis. …”
Publicado 2017
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31388“…Our goal was to select, examine, and characterize eight novel glycoside hydrolases that were previously detected in metagenomic libraries, to serve biotechnological applications with high performance. RESULTS: Here, eight glycosyl hydrolase family candidate genes were selected from metagenomes of wheat straw-degrading microbial consortia using molecular cloning and subsequent gene expression studies in Escherichia coli. …”
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31389por Kong, Wen, Fu, Xiao, Wang, Lei, Alhujaily, Ahmad, Zhang, Jingli, Ma, Fuying, Zhang, Xiaoyu, Yu, Hongbo“…The remarkable lignin-degradative potential makes VP attractive for biotechnological applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13068-017-0906-x) contains supplementary material, which is available to authorized users.…”
Publicado 2017
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31390“…This sporulation-negative mutant represents an improved industrial production strain for biotechnological processes otherwise impaired by the possibility of endospore formation. …”
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31391por Huo, Ying-Yi, Jian, Shu-Ling, Cheng, Hong, Rong, Zhen, Cui, Heng-Lin, Xu, Xue-Wei“…The characterization and mechanistic analyses of these two novel enzymes should provide a basis for further exploration of their potential biotechnological applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12934-018-0864-4) contains supplementary material, which is available to authorized users.…”
Publicado 2018
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31392por Kosa, Gergely, Zimmermann, Boris, Kohler, Achim, Ekeberg, Dag, Afseth, Nils Kristian, Mounier, Jerome, Shapaval, Volha“…A critical step in the development of such biotechnological applications is the selection of suitable strains for lipid production. …”
Publicado 2018
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31393por Moraes, Eduardo C., Alvarez, Thabata M., Persinoti, Gabriela F., Tomazetto, Geizecler, Brenelli, Livia B., Paixão, Douglas A. A., Ematsu, Gabriela C., Aricetti, Juliana A., Caldana, Camila, Dixon, Neil, Bugg, Timothy D. H., Squina, Fabio M.“…This work represents a pioneering study on lignin conversion and valorization strategies based on metagenomics, revealing several novel lignin conversion enzymes, aromatic-degrading bacterial genomes, and a novel bacterial strain of potential biotechnological interest. The validation of a biosynthetic route for vanillin synthesis confirmed the applicability of the targeted metagenome discovery approach for lignin valorization strategies. …”
Publicado 2018
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31394por da Costa, Elisabete, Azevedo, Vitor, Melo, Tânia, Rego, Andreia M., V. Evtuguin, Dmitry, Domingues, Pedro, Calado, Ricardo, Pereira, Rui, Abreu, Maria H., Domingues, Maria R.“…Overall, MS-based platforms represent a powerful tool to characterize lipid metabolism and target lipids along different life stages of algal species displaying complex life cycles (such as Porphyra dioica), contributing to their biotechnological application.…”
Publicado 2018
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31395por Ongey, Elvis L., Giessmann, Robert T., Fons, Michel, Rappsilber, Juri, Adrian, Lorenz, Neubauer, Peter“…Cultivating R. gnavus E1 is challenging, limiting high-quality production, further biotechnological development and therapeutic exploitation of RumA. …”
Publicado 2018
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31396“…There is the demand for searching plant species containing these iridoids or developing biotechnological methods to obtain the compounds. The present study investigated the effects of methyl jasmonate (MeJa, 50 μM), ethephon (Eth, 50 μM) and L-phenylalanine (L-Phe, 2.4 g/L of medium), added to previously selected variant of Murashige and Skoog medium (supplemented with plant growth regulators: 6-benzylaminopurine 1.0 mg/L, α-naphthaleneacetic acid 0.5 mg/L, gibberellic acid 0.25 mg/L) on the accumulation of harpagide and 8-O-acetyl-harpagide in Melittis melissophyllum L. agitated shoot cultures. …”
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31397“…Knowledge of the sequence of the plantaricin C coding region will help in studying its molecular components and allow their involvement in bacteriocin synthesis to be investigated, facilitating the use of the bacteriocin or its genetic elements in new biotechnological applications.…”
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31398Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCRpor Amorim, Lidiane Lindinalva Barbosa, Ferreira-Neto, José Ribamar Costa, Bezerra-Neto, João Pacífico, Pandolfi, Valesca, de Araújo, Flávia Tadeu, da Silva Matos, Mitalle Karen, Santos, Mauro Guida, Kido, Ederson Akio, Benko-Iseppon, Ana Maria“…The target genes analyzed, in turn, deserve functional evaluation due to their transcriptional regulation under stresses and biotechnological potential. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13007-018-0354-z) contains supplementary material, which is available to authorized users.…”
Publicado 2018
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31399por Guzina, Jelena, Chen, Wei-Hua, Stankovic, Tamara, Djordjevic, Magdalena, Zdobnov, Evgeny, Djordjevic, Marko“…We also find possible existence of a modified mechanism of CRISPR-associated small RNA action, which, interestingly, closely resembles the setup employed in biotechnological applications. Overall, our findings indicate that scaRNA:tracrRNA pairs are present in all subtypes of Type II systems, and point to an underlying connection with bacterial virulence. …”
Publicado 2018
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31400“…This possibly plays an important role in driving bacteriophage adaptation and therefore could be considered as beneficial. However, from biotechnological and experimental viewpoint, this might create some problems, and strongly argues for employing bacterial expression systems, stocked with proofreading mechanisms. …”
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