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6921“…In addition, types of cassettes and genes responsible for resistance to antibiotics for 22 multidrug resistant strains were determined. …”
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6922por Yang, Guanghua, Kramer, M. Gabriela, Fernandez-Ruiz, Veronica, Kawa, Milosz P., Huang, Xin, Liu, Zhongmin, Prieto, Jesus, Qian, Cheng“…Two distinct expression cassettes were inserted into a SIN-vector: one controlled by an endothelial lineage-specific, murine vascular endothelial cadherin (mVEcad) promoter for the expression of a transactivator, rtTA2S-M2; and the other driven by an inducible promoter, TREalb, for a firefly luciferase reporter gene. …”
Publicado 2015
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6923por McDougald, Devin S., Duong, Thu T., Palozola, Katherine C., Marsh, Anson, Papp, Tyler E., Mills, Jason A., Zhou, Shangzhen, Bennett, Jean“…These cells exhibit potent activation of proviral plasmids after transfection or after infection with AAV vectors delivering transgene cassettes carrying photoreceptor-specific promoters. …”
Publicado 2019
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6924“…These natural DNA transfer vehicles have been harnessed as experimental tools for stably introducing a wide variety of foreign DNA sequences, including selectable marker genes, reporters, shRNA expression cassettes, mutagenic gene trap cassettes, and therapeutic gene constructs into the genomes of target cells in a regulated and highly efficient manner. …”
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6925por Fan, Jianzhong, Cai, Heng, Fang, Youhong, He, Jintao, Zhang, Linghong, Xu, Qingye, Yang, Yunxing, Leptihn, Sebastian, Yu, Yunsong, Zhao, Dongdong, Hua, Xiaoting“…Despite the presence of mcr-9 cassettes, the strains were not rendered colistin resistant. …”
Publicado 2022
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6926“…Embryogenic calli of sugarcane were bombarded with both expression cassettes and selected on regeneration medium supplemented with phosphinothricin. …”
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6927por Sheppard, James, Land, Eric S., Toennisson, Tiffany Aurora, Doherty, Colleen J., Perera, Imara Y.“…Seedlings were grown for 5 days under continuous light in seed cassettes. The arrangement of the seed cassettes within each experimental container results in a gradient of fractional g (in the spinning rotor). …”
Publicado 2021
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6928por Delannoy Sotomayor, Andres Guillermo“…In preparation for Run-3 the BRIL project is performing a scheduled rebuild of two online luminometers, the Pixel Luminosity Telescope (PLT), one of four cassettes with four telescopes is shown in the photograph, and the Fast Beam Conditions Monitor (BCMF) The Run-2 results were extremely promising and are expected to yield best ever systematic and statistical uncertainties for precision physics measurements.…”
Publicado 2019
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6929
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6930“…In theory, up to ten single guide RNA (sgRNA) cassettes can be simultaneously assembled into the final binary vectors. …”
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6931por Allan, Christopher M., Procaccia, Shiri, Tran, Deanna, Tu, Yiping, Barnes, Richard H., Larsson, Mikael, Allan, Bernard B., Young, Lorraine C., Hong, Cynthia, Tontonoz, Peter, Fong, Loren G., Young, Stephen G., Beigneux, Anne P.“…To investigate the importance of SLURP2, we first examined Slurp2 knockout mice in which exon 2–3 sequences had been replaced with lacZ and neo cassettes. Slurp2(−/−) mice exhibited hyperkeratosis on the volar surface of the paws (i.e., PPK), increased keratinocyte proliferation, and an accumulation of lipid droplets in the stratum corneum. …”
Publicado 2015
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6932por Wang, Yu, Wei, Dongsheng, Zhu, Xiangyang, Pan, Jiao, Zhang, Ping, Huo, Liang, Zhu, Xudong“…We then demonstrated the spontaneous elimination of the system via a cis arrangement of the CRISPR-Cas9 expression cassettes to the recombination construct. After a system-mediated double crossover, the CRISPR-Cas9 cassettes were cleaved and degraded, which was validated by Southern blotting. …”
Publicado 2016
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6933por Jessop‐Fabre, Mathew M, Jakočiūnas, Tadas, Stovicek, Vratislav, Dai, Zongjie, Jensen, Michael K, Keasling, Jay D, Borodina, Irina“…The integration of linearized expression cassettes into defined genomic loci is facilitated by CRISPR/Cas9. …”
Publicado 2016
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6934por Heneghan, Mary N., Burns, Claire, Costa, Ana M. S. B., Burton, Kerry S., Challen, Michael P., Bailey, Andy M., Foster, Gary D.“…Three Spr1 gene silencing cassettes were constructed around sense, antisense and non‐translatable‐stop strategies (pGRsensehph, pGRantihph and pGRstophph). …”
Publicado 2016
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6935“…We also provided related genetic parts to assist in the construction of mutagenesis cassettes with a tetracycline-selectable marker. CONCLUSIONS: Our plasmid greatly simplifies the construction of Gene Doctoring donor plasmids and allows for the assembly of complex, multi-part insertion or deletion cassettes with a free choice of target sites and selection markers. …”
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6936por Yoshimi, Kazuto, Oka, Yuichiro, Miyasaka, Yoshiki, Kotani, Yuko, Yasumura, Misato, Uno, Yoshihiro, Hattori, Kosuke, Tanigawa, Arisa, Sato, Makoto, Oya, Manami, Nakamura, Kazuhiro, Matsushita, Natsuki, Kobayashi, Kazuto, Mashimo, Tomoji“…G0 KI mice carried NHEJ-dependent indel mutations at one targeting site that was designed at the intron region, and HDR-dependent precise KIs of the various donor cassettes spanning from 1 to 5 kbp, such as EGFP, mCherry, Cre, and genes of interest, at the other exon site. …”
Publicado 2020
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6937por Súnico, Victoria, Higuera, José Javier, Molina-Hidalgo, Francisco J., Blanco-Portales, Rosario, Moyano, Enriqueta, Rodríguez-Franco, Antonio, Muñoz-Blanco, Juan, Caballero, José L.“…In this paper, we took advantage of these innovative approaches to create four RNAi intragenic silencing cassettes by combining specific strawberry new promoters and pathogen defense-related candidate DNA sequences to increase strawberry fruit quality and resistance by silencing their corresponding endogenous genes, mainly during fruit ripening stages, thus avoiding any unwanted effect on plant growth and development. …”
Publicado 2021
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6938“…Other cassettes occurred in lower prevalence of below 5%. …”
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6939por Ghasemi, Elahe, Ferdosi-Shahandashti, Elaheh, Rajabnia, Mehdi, Sabbagh, Parisa, Maali, Amirhosein, Ferdosi-Shahandashti, Azadeh“…The present study was conducted for evaluating the prevalence of class I integron, determining the gene cassettes and antimicrobial resistance profile of Enterobacter spp. isolates from clinical samples in Babol, North of Iran. …”
Publicado 2021
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6940“…We list the factors influencing the expression of the cassettes, the expression of the integrase, and the various recombination reactions catalyzed by the integrase. …”
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