“…Transcription analysis revealed that most of these genes were not transcribed, particularly gene cassettes of class 1 integrons. The research revealed a high level of resistance associated with the metaphylactic treatment, persistence and circulation of resistance in bacterial populations. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we report the assignment of the codon to one for tryptophan and show that this can be used as an effective strategy for addressing a key problem in chloroplast engineering: namely, the assembly of expression cassettes in Escherichia coli when the gene product is toxic to the bacterium. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…They allow bacteria to capture, express and exchange antibiotic resistance genes embedded within gene cassettes. Acquisition of gene cassettes is catalysed by the class 1 integron integrase, a site-specific recombinase playing a key role in the integron system. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We found that the AdSDV harbors its NS and VP cassettes on two separate genome segments. Its vp segment acquired a phospholipase A2-encoding gene, vpORF3, via inter-subfamily recombination, coding for a non-structural protein. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…BACKGROUND: Diversity Generating Retroelements (DGRs) are genetic cassettes that can introduce tremendous diversity into a short, defined region of the genome. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here we report CRISPR/Cas9-mediated knock-in of DNA cassettes into the zebrafish genome at a very high rate by homology-independent double-strand break (DSB) repair pathways. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Lentivirus-based gene delivery vectors carrying multiple gene cassettes are powerful tools in gene transfer studies and gene therapy, allowing coexpression of multiple therapeutic factors and, if desired, fluorescent reporters. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…In particular, insertions of functional multi-transgene cassettes into suitable endogenous sequences will lead to novel applications. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The shared genes between these gene cassettes and their encoded proteins are distinguished by %G + C and protein sequence respectively. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two common signal sequences in TAT system (TorA and SufI) were added at the N-terminal of somatropin and the cassettes were co-expressed in E. coli BL21 (DE3) by a chaperone team including DnaK/J-GrpeE. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Integrons capture and rearrange promoterless gene cassettes in a unique recombination process involving the recognition of folded single-stranded DNA hairpins—so-called attC sites—with a strong preference for the attC bottom strand. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…However, we demonstrate that vector genome designs that carry dual sgRNA expression cassettes in tail-to-tail configurations lead to truncations. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Multiple target gene modifications were then carried out simultaneously via allelic exchange using various homologous recombinogenic DNA fragments to replace the sacBKan cassettes in the chromosomes of the intermediate strains. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Unexpectedly, we discovered plastid genome instability in transplastomic plants expressing hpRNAs, suggesting that dsRNA cassettes are preferable over hpRNA cassettes when designing PM-RNAi strategies. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To streamline the introduction of tagged genes and compartmental markers with powerful Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) - CRISPR-associated protein 9 (Cas9)-based genome editing tools, we constructed a Markerless Yeast Localization and Overexpression (MyLO) CRISPR-Cas9 toolkit with 3 components: (1) a set of optimized Streptococcus pyogenes Cas9-guide RNA expression vectors with 5 selectable markers and the option to either preclone or cotransform the gRNAs; (2) vectors for the one-step construction of integration cassettes expressing an untagged or green fluorescent protein/red fluorescent protein/hemagglutinin-tagged gene of interest at one of 3 levels, supporting localization and overexpression studies; and (3) integration cassettes containing moderately expressed green fluorescent protein- or red fluorescent protein-tagged compartmental markers for colocalization experiments. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we tested the suitability of a codon-optimized hyperactive piggyBac transposase (hyPBase) in mRNA form to deliver and integrate transgenic cassettes into the genome of the pantry moth Plodia interpunctella. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso