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  1. 6981
    “…We use these to generate improved synthetic circuits and optimise the co-expression of transfected cassettes, shedding light on how this can be useful for bioproduction and biotherapeutic applications. …”
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  2. 6982
    por Freund, B., Neubüser, C., Repond, J., Schlereth, J., Xia, L., Dotti, A., Grefe, C., Ivantchenko, V., Antequera, J.Berenguer, Calvo Alamillo, E., Fouz, M.C., Marin, J., Puerta-Pelayo, J., Verdugo, A., Brianne, E., Ebrahimi, A., Gadow, K., Göttlicher, P., Günter, C., Hartbrich, O., Hermberg, B., Irles, A., Krivan, F., Krüger, K., Kvasnicka, J., Lu, S., Lutz, B., Morgunov, V., Provenza, A., Reinecke, M., Sefkow, F., Schuwalow, S., Tran, H.L., Garutti, E., Laurien, S., Matysek, M., Ramilli, M., Schroeder, S., Bilki, B., Norbeck, E., Northacker, D., Onel, Y., Cvach, J., Gallus, P., Havranek, M., Janata, M., Kovalcuk, M., Kvasnicka, J., Lednicky, D., Marcisovsky, M., Polak, I., Popule, J., Tomasek, L., Tomasek, M., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., van Doren, B., Wilson, G.W., Kawagoe, K., Hirai, H., Sudo, Y., Suehara, T., Sumida, H., Takada, S., Tomita, T., Yoshioka, T., Bilokin, S., Bonis, J., Cornebise, P., Pöschl, R., Richard, F., Thiebault, A., Zerwas, D., Hostachy, J.Y., Morin, L., Besson, D., Chadeeva, M., Danilov, M., Markin, O., Popova, E., Gabriel, M., Goecke, P., Kiesling, C., der Kolk, N.van, Simon, F., Szalay, M., Corriveau, F., Blazey, G.C., Dyshkant, A., Francis, K., Zutshi, V., Kotera, K., Ono, H., Takeshita, T., Ieki, S., Kamiya, Y., Ootani, W., Shibata, N., Jeans, D., Komamiya, S., Nakanishi, H.
    Publicado 2016
    “…The thickness of each layer corresponded approximately to 0.29 radiation lengths or 0.034 nuclear interaction lengths, defined mostly by the copper and steel skins of the detector cassettes. This paper reports on measurements performed with this device in the Fermilab test beam with positrons in the energy range of 1 to 10 GeV. …”
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  3. 6983
    “…The thickness of each layer corresponded approximately to 0.29 radiation lengths or 0.034 nuclear interaction lengths, defined mostly by the copper and steel skins of the detector cassettes. This paper reports on measurements performed with this device in the Fermilab test beam with positrons in the energy range of 1 to 10 GeV. …”
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  4. 6984
    “…At all loci tested we obtained hESC and iPSC single-cell-derived clones carrying transgenic cassettes solely at the TALEN-specified location. Thus, TALENs mediate site-specific genome modifications in human pluripotent cells with comparable efficiency and precision as zinc finger nucleases (ZFNs).…”
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  5. 6985
    “…Here, we describe the packaging of tandem guide RNAs and single‐strand annealing‐based surrogate reporter cassettes into the CRISPR/CRISPR‐associated protein 9 vector, which increased gene‐editing efficiency by 4.94–6.31‐fold and simultaneously enriched the proportion of genetically modified cells. …”
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  6. 6986
    “…Here, we describe an elaborate procedure that allows the assembly of multiple gRNA expression cassettes into a vector of choice within a single step, termed ASAP(Adaptable System for Assembly of multiplexed Plasmids)-cloning. …”
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  7. 6987
    “…To simplify the construction of luciferase reporter cassettes with various-sized portions of the upstream region, genomic copies of BORIS were isolated using TAR cloning technology. …”
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  8. 6988
    “…The regions flanking the silent variant surface glycoprotein (VSG) gene cassettes showed extensive arrays of well-positioned nucleosomes, which may repress cryptic transcription initiation. …”
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  9. 6989
    “…Here, we describe a CRISPR/Cas9 system which can be conveniently developed as a CRISPR kit. SgRNA expression cassettes can be rapidly generated by one-step PCR using our CRISPR kit. …”
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  10. 6990
    “…However, in vegetative crop propagation, CRISPR/Cas9 expression cassettes cannot be segregated out in the resulting progenies, but must nevertheless be eliminated without leaving unnecessary sequences in the genome. …”
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  11. 6991
    “…There were also substantial changes in the major integron, attributed to gain of individual cassettes including copying from within, or loss of blocks of cassettes. …”
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  12. 6992
    “…An enormous variety and variability of sugar utilization gene cassettes were identified, with each strain harbouring between 25–53 cassettes, reflecting the high adaptability of L. paracasei to different niches. …”
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  13. 6993
  14. 6994
    “…By incorporating transposon-specific barcodes into the cassettes, we can evaluate how null mutations and changes in gene expression levels affect fitness in a single sequencing data set. …”
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  15. 6995
    “…In this work, the regulation of transcription of the individual repABC cassettes of the four RtTA1 plasmids was studied. The involvement of the RepA and RepB as well as parS-like centromere sites in this process was depicted, demonstrating some dissimilarity in expression of respective rep regions. …”
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  16. 6996
    “…Vectors are available as empty backbones and are fully flexible regarding the orientation of expression cassettes and addition of linkers between them, if required. …”
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  17. 6997
    “…In our recent study, we have shown that natural transformation can promote the transfer of transposons, IS elements, and integrons and gene cassettes, largely independent of the genetic relationship between the donor and recipient bacteria. …”
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  18. 6998
    “…Fast design optimization demands flexible interchangeable cassettes for endogenous gene silencing and tuneable expression. …”
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  19. 6999
    “…Herein, we describe the synthesis of suitably protected α- and β-rhamnosylated arginine amino acid “cassettes” that can be directly installed into rhamnosylated peptides. …”
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  20. 7000
    por Purcell, Henry, 1659-1695
    Publicado 1989
    CD Audiom
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