“…Whether high-level combinations of domains are conserved and transmitted as stable units (cassettes) through evolution is examined in the genomes of plant or metazoan species selected for their established position in the evolution of the respective lineages. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Class 1 integrons were detected in 10.2% (5/49) of these isolates and all contained gene cassettes aadA2 (0.65 kb). Two β-lactamase genes were detected among these isolates, and most of these isolates carried bla(TEM-1) (46/49), followed by bla(OXA-1)(4/49). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Among 19 Salmonella isolated from SH, high antibiotic resistance rates were found for nalidixic acid (19/19, 100%), tetracycline (19/19, 100%), ampicillin (18/19, 94.7%), and ciprofloxacin (13/19, 68.4%); class 1 integron was observed in two isolates (2/19, 10.5%), and gene cassettes included drfA17-aadA5 (1.7 kb, n = 1) and drfA1-aadA1 (1.4 kb, n = 1); bla(TEM-1) was the dominant β-lactamase gene (19/19, 100%), followed by bla(CTX-M-55) (2/19, 10.5%) and bla(CMY-2) (1/19, 5.3%). …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Very recently, Golden Gate based libraries were adapted to optimize single expression cassettes for recombinant proteins in P. pastoris. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Multi-locus sequence typing (MLST) can be used to analyze the homology among the drug resistance gene cassettes in Salmonella and determine the prevalence. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To explore this possibility, we have assessed the ability of self-assembling DNA nanoparticles to be constructed from complete gene cassettes that are capable of gene expression in vitro. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we leverage Golden Gate cloning to create the Fragmid toolkit, a modular set of CRISPR cassettes and delivery technologies, along with a web portal, resulting in a combinatorial platform that enables scalable vector assembly within days. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The approach was tested for conversion of genes into TetO-inducible/repressible expression cassettes. CONCLUSION: The simple introduction and tuning of any transcriptional control in the linear DNA product renders promoter activation and regulated gene studies simple and versatile.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Sequencing showed that the class 1 integron–bearing strain contained two distinct gene cassettes, sat-1 and aadA. Although three of the four class 1 integron–bearing strains harbored the aadA sequence, none of the strains was phenotypically resistant to streptomycin. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These toxins are classified into four major groups: the toxin complexes (Tcs), the Photorhabdus insect related (Pir) proteins, the “makes caterpillars floppy” (Mcf) toxins and the Photorhabdus virulence cassettes (PVC); the mechanisms however of toxin secretion are not fully elucidated. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…As a result, the replacement of stimulus and promotor cassettes to rewire TCS is well defined exploiting the alignments given here. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…PAHs were measured in personal and stationary sampling by 40 mm Millipore cassettes, for adsorption of both gaseous and particle-bound PAHs. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These promoter libraries were then combined to create and optimize a series of IPTG inducible cassettes. The resultant induction system had a 48-fold dynamic range and was shown to out-perform P(trc) constructs. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Also in these reporter plasmids the sarA-P1 promoter can be replaced by any other promoter of interest for expression studies. Cassettes from the replication plasmids can be readily swapped with the integration vector. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The large size and relative complexity of such CRISPR transgene cassettes impede their broad implementation, especially in gene therapy applications with viral vectors that have a limited packaging capacity. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…These elements are able to acquire gene cassettes, which can carry antibiotic resistance factors, by site-specific recombination mechanism. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To overcome these limitations, expression cassettes were embedded into transposon-derived donor vectors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we describe a modular recombinase-based gene circuit architecture, comprising tandem gene perturbation cassettes (GPCs), that enables the sequential expression of multiple genes in a defined temporal order by alternating treatment with just two orthogonal ligands. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…By streamlining the vector backbone and insert design, these publicly available vectors allow easy interchangeability of the independent building blocks, such as different promoters, fluorescent proteins, surface markers and antibiotic resistance cassettes. We validated these vectors and tested the optimal promoters for in vitro and in vivo overexpression and knockdown of the murine T cell antigen receptor. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso