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  1. 7061
    “…To overcome this, we developed insertion pool sequencing (iPool‐Seq). iPool‐Seq allows direct analysis of colonized tissue due to high specificity for insertional mutant cassettes. Here, we describe detailed protocols for infection as well as genomic DNA extraction to study the interaction between the corn smut fungus Ustilago maydis and its host maize. …”
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    Insertion Pool Sequencing for Insertional Mutant Analysis in Complex Host‐Microbe Interactions
  2. 7062
    por Verdi, Giuseppe, 1813-1901
    Publicado 1991
    CD Audiom
    La traviata
  3. 7063
    por Puccini, Giacomo, 1858-1924
    Publicado 1987
    CD Audiom
    Madama Butterfly
  4. 7064
    por Hooker, John Lee
    Publicado 1991
    CD Audiom
    The ultimate collection, 1948-1990
  5. 7065
    por Sefkow, Felix, Simon, Frank
    Publicado 2018
    “…The front-end chips are integrated into the active layers of the calorimeter and are designed for minimizing power consumption by rapidly cycling the power according to the beam structure of a linear accelerator. 38 layers of the sampling structure are equipped with cassettes containing 576 single channels each, arranged on readout boards and grouped according to the 36 channel readout chips. …”
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    A highly granular SiPM-on-tile calorimeter prototype
  6. 7066
    “…Furthermore, the vector was adapted for convenient ligation-free transfer of microRNA cassettes from public libraries. This conditional knockdown-system should prove useful for many research and gene therapeutic applications.…”
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    Inducible microRNA expression by an all-in-one episomal vector system
  7. 7067
    “…The cloning of Lr47 expands the number of leaf rust resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.…”
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    Cloning of the wheat leaf rust resistance gene Lr47 introgressed from Aegilops speltoides
  8. 7068
    “…The eCISs comprise the R-tailocins produced by various bacterial species as well as related phage tail-like structures such as the antifeeding prophages (Afps) of Serratia entomophila, the Photorhabdus virulence cassettes (PVCs), and the metamorphosis-associated contractile structures (MACs) of Pseudoalteromonas luteoviolacea. …”
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    Evolutionary and ecological role of extracellular contractile injection systems: from threat to weapon
  9. 7069
    “…RESULTS: Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron. …”
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    Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element
  10. 7070
    “…Discrete mutations were observed, leading to new chemicals that we isolated from animals. Functional cassettes encoding diverse chemicals are exchanged among a single population of symbiotic P. didemni that spans the tropical Pacific, providing the host animals with a varying arsenal of secondary metabolites.…”
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    Variation in Tropical Reef Symbiont Metagenomes Defined by Secondary Metabolism
  11. 7071
    “…Here, we undertake a comprehensive comparison of widely used proxy reporters including both CreERT2 and EGFP cassettes targeted to the Lgr5, Bmi1, and Hopx loci. …”
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    Single-Cell Analysis of Proxy Reporter Allele-Marked Epithelial Cells Establishes Intestinal Stem Cell Hierarchy
  12. 7072
    “…Here we describe the construction of improved antibiotic-resistance cassettes that specify resistance to kanamycin, chloramphenicol or trimethoprim effectively in the Bcc and related species. …”
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    An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia
  13. 7073
    “…The vectors were used to (i) knock in heterologous phytase, xylanase and lipase expression cassettes, (ii) increase the phytase, xylanase and lipase gene copy number to 13, 5, and 5, respectively, with vector introduction and (iii) engineer the secretion pathway by co-overexpressing secretion helper factors (Sly1p and Sec1p) without introducing selectable markers, giving a phytase field of 0.833 g/L. …”
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    Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
  14. 7074
    “…Additionally, we identify criteria that are important for the effectiveness of a particular miRNA for such applications and we also discuss factors that have to be taken into consideration when designing miRNA-regulated expression cassettes.…”
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    MicroRNA-Regulated Gene Delivery Systems for Research and Therapeutic Purposes
  15. 7075
    “…Here we report the generation of a modified hESC line harboring two suicide gene cassettes, whose expression results in cell death in the presence of specific pro-drugs. …”
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    A Double Fail-Safe Approach to Prevent Tumorigenesis and Select Pancreatic β Cells from Human Embryonic Stem Cells
  16. 7076
    “…Our approach uses short homology of 24–48 bp to drive targeted integration of DNA reporter cassettes by homology-mediated end joining (HMEJ) at high frequency at a double strand break in the targeted gene. …”
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    Efficient targeted integration directed by short homology in zebrafish and mammalian cells
  17. 7077
    “…Complex phylogenetics support the concept that gene cassettes encoding the subunits for these pumps are commonly acquired by horizontal gene transfer. …”
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    A noncanonical chaperone interacts with drug efflux pumps during their assembly into bacterial outer membranes
  18. 7078
    “…Two selected events of transgenic sugar beet carrying cassettes inducing RNA silencing mechanism, 219-T3:S3-13.2 (S3) and 6018-T3:S6-44 (S6), were shown to inhibit propagation of Beet Necrotic Yellow Vein Virus, the causative agent. …”
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    Investigation of Possible Changes Induced by RNA Silencing in Some Leaf Metabolites of Transgenic Sugar Beet Events
  19. 7079
    “…Here we present a high‐throughput gene knockout method that combines a highly reproducible fusion PCR method to enable generation of gene replacement cassettes with a multiwell format transformation procedure. …”
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    High‐Throughput Gene Replacement in Aspergillus fumigatus
  20. 7080
    “…Furthermore, class 1 integrons were detected in 38 % (48/128) of P. aeruginosa and Acinetobacter, 37 % (26/71) of which were in clinical isolates and 39 % (22/57) in environment isolates. Gene cassettes were found in 25 % (12/48) of integron-positive isolates. …”
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    Carbapenem resistant Pseudomonas aeruginosa and Acinetobacter baumannii at Mulago Hospital in Kampala, Uganda (2007–2009)
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