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  1. 7081
    por Brahms, Johannes, 1833-1897
    Publicado 1990
    CD Audiom
    Piano quartets
  2. 7082
    por Rossini, Gioacchino, 1792-1868
    Publicado 1986
    CD Audiom
    Guglielmo Tell
  3. 7083
    “…Finally, we describe how insertional cassettes can be erroneously mutated during insertion and suggest strategies to select for lines that are modified as designed.…”
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    Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas
  4. 7084
    “…Yeast strains with chromosomally integrated versions of tagged transcription factor genes were generated using N- or C-terminal biotin-tagging cassettes. ChIP experiments with biotinylated Rbp3p, a RNA polymerase II subunit, showed that Rbp3p-binding could even be detected at weakly expressed genes. …”
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    The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation
  5. 7085
    “…The biosensor was based on 21-mer single-stranded oligonucleotide (ssDNA probe) specific to either 35S promoter or nos terminator, which are frequently present in transgenic DNA cassettes. ssDNA probe was covalently attached by 5′-phosphate end to amino group of cysteamine self-assembled monolayer (SAM) on gold electrode surface with the use of activating reagents – water soluble 1-ethyl-3(3′-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxy-sulfosuccinimide (NHS). …”
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    Application of DNA Hybridization Biosensor as a Screening Method for the Detection of Genetically Modified Food Components
  6. 7086
    “…To increase throughput, we combined drugs into cassettes for simultaneous measurement of multiple compounds. …”
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    A High-Throughput Cell-Based Method to Predict the Unbound Drug Fraction in the Brain
  7. 7087
    por Fougeroux, Cyrielle, Holst, Peter J.
    Publicado 2017
    “…Here, we will first give an overview of the basic properties of vectored vaccines, followed by an introduction to the characteristics of adenoviral vectors and previously tested modifications of the vector backbone and expression cassettes, with a focus on how they can contribute to increased vaccine cost-effectiveness. …”
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    Future Prospects for the Development of Cost-Effective Adenovirus Vaccines
  8. 7088
    por Lundstrom, Kenneth
    Publicado 2020
    “…Viral gene silencing by targeting the RNAi pathway showed efficient suppression of hepatitis B virus replication by adeno-associated virus (AAV)-based delivery of RNAi hepatitis B virus (HBV) cassettes. Hepatitis C virus replication has been targeted by short hairpin RNA molecules expressed from lentivirus vectors. …”
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    Are Viral Vectors Any Good for RNAi Antiviral Therapy? †
  9. 7089
    “…The structural uniqueness of allopeptimicins is reflected in a biosynthetic gene cluster showing a mosaic structure, with dedicated gene cassettes devoted to formation of specialized precursors and modular assembly lines related to those from different pathways.…”
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    Allopeptimicins: unique antibacterial metabolites generated by hybrid PKS-NRPS, with original self-defense mechanism in Actinoallomurus
  10. 7090
    “…Furthermore, we optimized the subcellular targeting by controlling expression levels using a collection of different promoter cassettes; the MoClo strategy allows it to generate arrays of expression plasmids in parallel to optimize gene expression levels and reliable targeting for each given protein and cellular compartment. …”
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    A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast Saccharomyces cerevisiae
  11. 7091
    “…The approach is compatible with iPSC genetic engineering, including inserting targeted transgenic reporter cassettes to track the xenografted human cells, enabling the testing of novel treatment and viral tracking strategies in a comparatively simple and cost-effective way vivo model for neuroHIV.…”
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    HIV-1 infection of genetically engineered iPSC-derived central nervous system-engrafted microglia in a humanized mouse model
  12. 7092
    “…Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.…”
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    Complete Genomic Sequence of Bacteriophage Felix O1(†)
  13. 7093
    “…Previously, we described a large collection of Minos-Mediated Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a new exon that encodes a protein tag when the MiMIC is inserted in a codon intron (Nagarkar-Jaiswal et al., 2015). …”
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    A genetic toolkit for tagging intronic MiMIC containing genes
  14. 7094
    “…We demonstrate that exogenous RNA domains can be functionally appended onto the CRISPR scaffold at multiple insertion points, allowing the construction of Cas9 complexes with protein-binding cassettes, artificial aptamers, pools of random sequences, and RNAs up to 4.8 kilobases in length, including natural lncRNAs. …”
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    CRISPR Display: A modular method for locus-specific targeting of long noncoding RNAs and synthetic RNA devices in vivo
  15. 7095
    “…The transplastomic plants were then combinatorially supertransformed with cassettes for all additional enzymes known to affect flux through the artemisinin pathway. …”
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    A new synthetic biology approach allows transfer of an entire metabolic pathway from a medicinal plant to a biomass crop
  16. 7096
    “…Here we describe a modular system entitled CRISPaint (CRISPR-assisted insertion tagging) that allows precise and efficient integration of large heterologous DNA cassettes into eukaryotic genomes. CRISPaint makes use of the CRISPR-Cas9 system to introduce a double-strand break (DSB) at a user-defined genomic location. …”
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    CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism
  17. 7097
    “…The ability to incorporate multiple single guide RNA (sgRNA) cassettes together with Cas9 gene expression in one AAV vector could greatly enhance the efficiency. …”
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    CRISPR/Cas9 with single guide RNA expression driven by small tRNA promoters showed reduced editing efficiency compared to a U6 promoter
  18. 7098
    “…These pH‐dependent DNAzymes could be used as molecular cassettes in biotechnology or nanotechnology, in molecular processes that consist of several steps. …”
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    Characterization of Highly Efficient RNA‐Cleaving DNAzymes that Function at Acidic pH with No Divalent Metal‐Ion Cofactors
  19. 7099
    “…Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. …”
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    A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses
  20. 7100
    “…Here we show the encapsulation of two million primary B cells into picoliter-sized droplets, where their cognate V genes are fused in-frame to form a library of scFv cassettes. We used this approach to construct natively paired phage-display libraries from healthy donors and drove selection towards cross-reactive antibodies targeting influenza hemagglutinin. …”
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    Recombinant human B cell repertoires enable screening for rare, specific, and natively paired antibodies
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