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  1. 7121
    “…We describe tRNA(Lys3)-shRNA chimeric expression cassettes which produce siRNAs with comparable efficacy and strand selectivity to U6-expressed shRNAs, and show that their activity is consistent with processing by endogenous 3′ tRNAse. …”
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    Optimization and characterization of tRNA-shRNA expression constructs
  2. 7122
    “…The porcine 7SK (po7SK) promoter was cloned and a one-step PCR strategy used to construct shRNA expression cassettes. The 7SK promoter activity was quantified by knockdown of the exogenous reporter gene encoding the enhanced green fluorescent protein (EGFP). …”
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    Identification and characterisation of the porcine 7SK RNA polymerase III promoter for short hairpin RNA expression
  3. 7123
    “…The trpA-E genes along with ‘regulatory’ elements were delivered as individual recombination cassettes in a synthetic integron platform. Integrase-mediated recombination generated thousands of genetic combinations overnight. …”
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    The synthetic integron: an in vivo genetic shuffling device
  4. 7124
    “…Targeting vectors that replace exons 7 and 8 of the hprt gene with neomycinR/thymidine kinase selection cassettes were electroporated into male Fisher F344 and Sprague Dawley rat ES cells. …”
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    Efficient Gene Targeting by Homologous Recombination in Rat Embryonic Stem Cells
  5. 7125
    “…Here we provide critical details about the underlying recombineering and transgenesis method, new cassettes for tagging endogenous loci and information on important parameters that will allow Drosophila researchers to target members of other gene families.…”
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    Combining recombineering and ends-out homologous recombination to systematically characterize Drosophila gene families: Rab GTPases as a case study
  6. 7126
    “…The resultant assemblies contain arrays of DNA cassettes separated by recombination sites, which can be used to manipulate the assembly by further recombination. …”
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    Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination
  7. 7127
    “…Insertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). …”
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    Isolation of Human Colon Stem Cells Using Surface Expression of PTK7
  8. 7128
    “…Moreover, we implemented dual-host-specific cassettes enabling multiprotein expression in insect and mammalian cells using a single reagent. …”
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    Highly efficient baculovirus-mediated multigene delivery in primary cells
  9. 7129
    “…Here we report a genome survey of 3,400 actinomycetes, identifying 81 strains that harbor genes encoding the enediyne polyketide synthase cassettes that could be grouped into 28 distinct clades based on phylogenetic analysis. …”
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    Strain Prioritization and Genome Mining for Enediyne Natural Products
  10. 7130
    “…The systems are based on the Escherichia coli Tn10-derived tet regulatory system and consist of a strong tet operator (tetO)-containing mycobacterial promoter, expression cassettes for the repressor TetR and the chemical inducer ATc. …”
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    Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor
  11. 7131
    “…Toxin–antitoxin (TA) genes were first reported in plasmids and were considered expendable genetic cassettes involved in the stable maintenance of the plasmid replicon by interfering with growth and/or viability of bacteria in which the plasmid was lost. …”
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    The Importance of the Expendable: Toxin–Antitoxin Genes in Plasmids and Chromosomes
  12. 7132
    “…Sr21 can be a valuable component of transgenic cassettes or gene pyramids combining multiple resistance genes against Ug99.…”
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    Identification and characterization of wheat stem rust resistance gene Sr21 effective against the Ug99 race group at high temperature
  13. 7133
    “…We report the expression of hundreds of genes not previously reported. Finally, inserted cassettes can be replaced with GFP or any DNA. These stocks comprise a powerful resource for assessing gene function.…”
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    A gene-specific T2A-GAL4 library for Drosophila
  14. 7134
    “…Employing this marker–gene-recycling system, Coccomyxa strains devoid of foreign DNA except the 34-bp loxP sequence, which overexpressed an acyl-(acyl-carrier-protein) thioesterase gene, and a type-2 diacylglycerol acyltransferase gene, were constructed by the sequential introduction of two expression cassettes for the respective genes. One of the resulting strains showed 1.4-fold higher lipid productivity than the wild-type strain. …”
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    Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa
  15. 7135
    “…Comparative analysis of the plasmid architecture and gene inventory revealed that p866 shares its plasmid backbone with previously described IncB/O/K/Z-type Shigella spp. and Escherichia coli plasmids, but is differentiated by the insertion of antibiotic resistance cassettes, which we found associated with mobile genetic elements such as Tn3, Tn7, and Tn10. …”
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    Closed Genome and Comparative Phylogenetic Analysis of the Clinical Multidrug Resistant Shigella sonnei Strain 866
  16. 7136
    “…Previously described strategies for CRISPR-Cas9 genome editing in B. subtilis have involved chromosomal integrations of Cas9 and single guide RNA expression cassettes, or construction of large plasmids for simultaneous transformation of both single guide RNA and donor DNA. …”
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    CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis
  17. 7137
    por Merlin, Simone, Follenzi, Antonia
    Publicado 2019
    “…In this sense, the use of cell-type-specific promoters and microRNA target sequences (miRTs) in gene transfer expression cassettes have allowed for a restricted expression after gene transfer in several studies. …”
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    Transcriptional Targeting and MicroRNA Regulation of Lentiviral Vectors
  18. 7138
    “…The reporter, consisting of four main cassettes, simultaneously measures the effects of a candidate regulator on cis-SAGe and canonical splicing. …”
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    A cell-based splicing reporter system to identify regulators of cis-splicing between adjacent genes
  19. 7139
    “…Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. …”
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    Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium
  20. 7140
    “…We report the first extensive genomic characterization of microbiomes, pathogens and antibiotic resistance cassettes in a tertiary-care hospital, from repeated sampling (up to 1.5 years apart) of 179 sites associated with 45 beds. …”
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    Cartography of opportunistic pathogens and antibiotic resistance genes in a tertiary hospital environment
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