“…Although a HAC theoretically incorporates multiple gene expression cassettes of unlimited DNA size, its application has been limited because the conventional gene-loading system accepts only one gene-loading vector (GLV) into a HAC. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Association of integron with bla (NDM-1) and presence of gene cassettes were assessed by PCR. Genetic linkage of NDM gene with ISAba125 was determined and in negative cases linkage in upstream region was mapped by inverse PCR. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Promoters from two of these candidates, vitellogenin receptor and nanos, were used in independent transgenic cassettes for the expression of artificial microRNAs against suspected mosquito maternal-effect genes, discontinuous actin hexagon and myd88. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…The application of transcription factor activated luciferase reporter cassettes in vitro is widespread but potential for in vivo application has not yet been realized. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Furthermore, the assembly with the long nanopore reads presents a much more complete representation of the features of the genome and correctly assembles gene cassettes, rRNAs, transposable elements, and other genomic features that were almost entirely absent in the Illumina-only assembly.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…This system enables growth at 37 °C, thereby facilitating removal of integrated antibiotic cassettes and deletion of additional genes in the same day. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Additionally, the difference in particle distribution with different expression cassettes implies a recombinant vector-dependent processing mechanism which should be taken into account during process development.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…For example, the spatially- and/or temporally-restricted transduction of specific neuronal cell populations is now routinely achieved using the combination of Cre-driver mice and stereotaxic-based delivery of AAV expressing Cre-dependent cassettes. We predict that the experimental application of these tools, including creative combinatorial approaches and the development of even newer reagents, will prove necessary for a complete understanding of the neuronal circuits subserving most neurobiological functions, including the regulation of sleep and wake.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…On the respective SCCmec cassettes, the CC5 isolates differed clearly within this decade and CC5/SCCmecI, the South German MRSA, predominant in 2002, was replaced by CC5/SCCmecII, the Rhine-Hesse MRSA in 2012. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Two signal sequences, TorA and SufI are used at N-terminal of human growth hormone (hGH) bearing DsbA gene sequence at C-terminal to enhance folding. The synthetic cassettes including the signal sequence, hGH and DsbA were transformed into E. coli BL21 (DE3) to study the effect of signal sequence and DsbA chaperone on translocation and folding of the protein. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…TirS invariably localizes within the staphylococcal chromosomal cassettes (SCC) containing the fusC gene for fusidic acid resistance but not always carrying the mecA gene. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To this end, the systematic introduction of seven transgenic cassettes into soya bean, and the molecular and phenotypic evaluation of the derived novel events are described.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…To reduce the off-target cleavage frequency and increase the versatility of our knock-in system, we constructed a universal donor vector and an expression vector containing Cas9 with enhanced specificity and tandem sgRNA expression cassettes. We demonstrated that the second version of our system has improved usability.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We used a SKC BioSampler with a BioLite Air Sampling Pump (run for 60 min at 8 L/min) and SKC AirChek TOUCH personal air samplers with polytetrafluoroethylene Teflon filter cassettes (run for 180 min at 5 L/min). The aerosol specimens and controls were studied with molecular assays for influenza A virus, influenza B virus, adenoviruses, and coronaviruses. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…However, toxicity of Pol III cassettes and short duration of silencing by effectors of the RNAi pathway may limit anti-HBV therapeutic utility. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Limited duration of transgene expression, insertional mutagenesis, and size limitations for transgene cassettes pose challenges and risk factors for many gene therapy vectors. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Here, we describe a molecular toolkit for rapid preparation of modified λ-DNA. A set of PCR cassettes facilitates the introduction of recombinant DNA sequences into the λ-phage genome with 90–100% yield. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We have evaluated luciferase bioimaging in conscious, unrestrained mice after neonatal intracranial or intravascular administration of lentiviral, luciferase reporter cassettes (biosensors); we present real-time analyses from the first day of life to adulthood. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…Our results suggest that IL-22 expressing lactobacilli may potentially be a useful mucosal therapeutic agent for the treatment of GVHD, provided that chromosomal integration of the IL-22 expression cassettes can be achieved. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12934-017-0762-1) contains supplementary material, which is available to authorized users.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We identify three splice cassettes and an alternative transcriptional start site in AplCCal1. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso