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  1. 7321
    “…These properties were achieved by introducing the cry1B.868 and cry1Da_7 expression cassettes. The molecular characterisation data and bioinformatic analyses do not identify issues requiring food/feed safety assessment. …”
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    Assessment of genetically modified maize MON 95379 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2020‐170)
  2. 7322
    “…According to the results, the internal gene cassettes of nine strains had not only undergone reassortment with the H1, H2, H4, H6, and H7 subtypes, which circulate in poultry and mammals, but also with H10N8, which circulates in wild birds in the natural environment. …”
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    A Characterization and an Evolutionary and a Pathogenicity Analysis of Reassortment H3N2 Avian Influenza Virus in South China in 2019–2020
  3. 7323
    “…Computational analysis of the T6SS1 gene cluster revealed that antibacterial effector and immunity proteins are encoded within three genomic islands that also harbour arrays of orphan immunity genes or toxin and immunity cassettes. Functional analyses indicated that VgrG, a specialized antibacterial effector, contains a C‐terminal catalytically active glucosaminidase domain that is used to degrade prey peptidoglycan. …”
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    An antibacterial T6SS in Pantoea agglomerans pv. betae delivers a lysozyme‐like effector to antagonize competitors
  4. 7324
    “…Pathfinder plasmids combine three different broad-host-range origins of replication with multiple antibiotic resistance cassettes and reporters, so that sets of parts can be rapidly screened through multiplex conjugation. …”
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    The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing Orbaceae
  5. 7325
    “…The initial fragment, sulfaguanidine, bound at the interface between the two helicase cassettes of BRR2 in one crystal form. Second-generation compounds devised by structure-guided docking were probed for their binding to BRR2 in a second crystal form, in which the original fragment-binding site was altered due to a conformational change. …”
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    Conformation-dependent ligand hot spots in the spliceosomal RNA helicase BRR2
  6. 7326
    “…A similar down regulation phenotype is displayed by transactivator-deficient cell variants, but this phenotype can be rescued by transfecting the cells with expression cassettes encoding hepatocyte nuclear factor-1α (HNF-1α) or HNF-4. …”
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    Formation of a large, complex domain of histone hyperacetylation at human 14q32.1 requires the serpin locus control region
  7. 7327
    “…RESULTS: Among the 734 filled medication cassettes, 179 errors were detected corresponding to a total of 7249 correctly fulfilled and omitted unit doses. …”
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    Frequency, Types, and Potential Clinical Significance of Medication-Dispensing Errors
  8. 7328
    por Sliva, Katja, Schnierle, Barbara S
    Publicado 2010
    “…Selective gene silencing by RNAi can be achieved essentially by two nucleic acid based methods: i) cytoplasmic delivery of short double-stranded (ds) interfering RNA oligonucleotides (siRNA), where the gene silencing effect is only transient in nature, and possibly not suitable for all applications; or ii) nuclear delivery of gene expression cassettes that express short hairpin RNA (shRNA), which are processed like endogenous interfering RNA and lead to stable gene down-regulation. …”
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    Selective gene silencing by viral delivery of short hairpin RNA
  9. 7329
    “…Methotrexate-driven transgene amplification of genetic cassettes that code full-length and truncated variants of FVIII under the control of the CMV promoter was studied. …”
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    Stable Expression of Recombinant Factor VIII in CHO Cells Using Methotrexate-Driven Transgene Amplification
  10. 7330
    por Walia, Rupali, Chaconas, George
    Publicado 2013
    “…The vls locus, carried on a 29 kb linear plasmid (lp28-1) in the type strain B31, carries 15 silent cassettes from which information is unidirectionally transferred into the expression locus, vlsE. …”
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    Suggested Role for G4 DNA in Recombinational Switching at the Antigenic Variation Locus of the Lyme Disease Spirochete
  11. 7331
    por Maekawa, Hiromi, Kaneko, Yoshinobu
    Publicado 2014
    “…Two silent mating type cassettes, in addition to an active MAT locus, are essential components of the mating type switching mechanism. …”
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    Inversion of the Chromosomal Region between Two Mating Type Loci Switches the Mating Type in Hansenula polymorpha
  12. 7332
    “…Intratumoral administration of adenovirus vectors carrying p53 trans-splicing cassettes suppressed the growth of tumor xenografts. …”
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    Functional repair of p53 mutation in colorectal cancer cells using trans-splicing
  13. 7333
    “…By concatemerizing multiple gRNA expression cassettes, we generated a ‘gRNA concatemer vector’. …”
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    Simultaneous paralogue knockout using a CRISPR-concatemer in mouse small intestinal organoids
  14. 7334
    “…Compellingly, the conserved upstream region and variable downstream region of vaa correlates with the genetic structure of vaa itself which consists of a conserved 5' end and a variable 3' end containing a variable number of exchangeable sequence cassettes. CONCLUSION: Our data demonstrate that the vaa locus contains a divergent genetic islet, and indicate pronounced intraspecies recombination. …”
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    The vaa locus of Mycoplasma hominis contains a divergent genetic islet encoding a putative membrane protein
  15. 7335
    “…To create and characterise a stably integrated expression system for DT40 cells, we constructed integration cassettes for three commonly used promoter elements; CMV (cytomegalovirus), CBA (chicken beta actin) or CAG (a hybrid promoter containing the CMV enhancer and chicken beta actin promoter), and used these to stably integrate a TOPBP1 transgene at the OVA locus, a transcriptionally silent locus commonly used in DT40. …”
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    Characterisation of a stably integrated expression system for exogenous protein expression in DT40
  16. 7336
    “…DESIGN AND METHODS: Blood samples from the delivery room (n=66) and from the ED (n=85) were analyzed on two POCT devices, the StatStrip-Lactate (Nova Biomedical) and the iSTAT-1 (CG4+ cassettes, Abbott), and compared to the routine laboratory analyzer (ABL-735, Radiometer). …”
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    Lactate point-of-care testing for acidosis: Cross-comparison of two devices with routine laboratory results
  17. 7337
    “…A limitation of AAV is the carrying capacity (∼5 kb) available for genes and regulatory cassettes (RCs). These size constraints are problematic for the 2.2-Mb dystrophin gene. …”
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    Development of Novel Micro-dystrophins with Enhanced Functionality
  18. 7338
    “…The initial codons of these genes were changed to random nucleotides NNN, and the gene cassettes were assembled into vectors via an optimized strategy based on type II restriction enzymes. …”
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    Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli
  19. 7339
    “…Here we demonstrate the use of a previously developed CRISPR/Cas9 gene editing protocol for the insertion of ILTV gD-gI and the H9N2 AIV hemagglutinin expression cassettes into the distinct locations of the recombinant HVT-IBDV VP2 viral genome, to generate the triple insert HVT-VP2-gDgI-HA recombinant vaccine. …”
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    Generation of A Triple Insert Live Avian Herpesvirus Vectored Vaccine Using CRISPR/Cas9-Based Gene Editing
  20. 7340
    “…One of the main routes of resistance development is the transfer of genes containing antibiotic resistance cassettes. Gene transfer can be done through horizontal transfer of genes: transduction, conjugation, and transformation. …”
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    Positive and Negative Effects of Metal Oxide Nanoparticles on Antibiotic Resistance Genes Transfer
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