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  1. 7381
    “…These were later validated by quantificational real-time polymerase chain reaction. Viral cassettes were created to induce expression of differentially expressed genes in the primed cells through lentiviral transduction. …”
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    Characterization and genetic manipulation of primed stem cells into a functional naïve state with ESRRB
  2. 7382
    “…Therefore, the underlying mechanisms of quercetin in improving RCT may be partially due to the elevated cholesterol accepting ability of HDL, the increased expression levels of proteins related to RCT, such as ATP-binding cassettes (ABC) A1 and G1, and the improved antioxidant activity of HDL. …”
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    Quercetin improves macrophage reverse cholesterol transport in apolipoprotein E-deficient mice fed a high-fat diet
  3. 7383
    “…We administered lentiviral transcription factor activated luciferase/eGFP reporter (TFAR) cassettes to neonatal mice enabling longitudinal TFAR profiling by continued bioimaging throughout the lives of the animals and following pBDL in adulthood. …”
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    Longitudinal in vivo bioimaging of hepatocyte transcription factor activity following cholestatic liver injury in mice
  4. 7384
    “…All strains received two copies of the pTEF-oPAI or pPOX-oPAI expression cassettes; PAI encodes linoleic acid isomerase in Propionibacterium acnes. …”
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    A metabolic engineering strategy for producing conjugated linoleic acids using the oleaginous yeast Yarrowia lipolytica
  5. 7385
    “…This system takes advantage of employing a single expression vector which consists of multiple semi-independent expressing cassettes for the protein co-expression thereby overcoming the limitations of using multiple independent expressing plasmids. …”
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    Once for All: A Novel Robust System for Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in Plants
  6. 7386
    “…In the present study, three PB transposon donor vectors containing both heavy and light chains were constructed, one contained independent expression cassettes while the others utilized either an Internal Ribosome Entry Site (IRES) or 2A element to express mAb. …”
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    Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design
  7. 7387
    “…Heterogeneity in base composition and codon preference has long been the subject of investigation of topics ranging from phylogenetic distortion to the design of overexpression cassettes for transgenic expression. From the overexpression point of view, it is critical to systematically analyze the codon usage patterns of the organelle genomes. …”
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    ChloroMitoCU: Codon patterns across organelle genomes for functional genomics and evolutionary applications
  8. 7388
    por Alaei, Parham, Spezi, Emiliano
    Publicado 2012
    “…The beams generated by Elekta X‐ray volume imaging (XVI) kilovoltage CBCT (kV CBCT) system for various cassettes and filters have been modeled in the Philips Pinnacle treatment planning system (TPS) and used to compute dose to stack and anthropomorphic phantoms. …”
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    Commissioning kilovoltage cone‐beam CT beams in a radiation therapy treatment planning system
  9. 7389
    “…Here, we describe the construction of a barcoded-library of 133 heterozygous TF deletion mutants and deletion cassettes for designed to facilitate complex haploinsufficiency-based genetic interaction studies of the TF networks in C. albicans. …”
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    Systematic Complex Haploinsufficiency-Based Genetic Analysis of Candida albicans Transcription Factors: Tools and Applications to Virulence-Associated Phenotypes
  10. 7390
    “…We took advantage of Golden Gate approach, for construction of expression cassettes, and micro-volume enzymatic assays, for functional screening of large libraries of recombinant strains. …”
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    Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags
  11. 7391
    “…BACKGROUND: Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been selected as key targets, allowing the coverage of most of GMO, EU authorized or not. …”
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    Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes
  12. 7392
    “…Via p5 promoter expression kinetics and strand specific RNA-Seq analysis of wtAAV2, rAAV and Rep2/Cap2 cassettes in the baculovirus context we could demonstrate that wtAAV2 native promoters, p5, p19 and p40 are all active in the context of the baculovirus system and lead to the expression of different proteins and peptides. …”
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    Genetics instability of wtAAV2 genome and AAV promoter activities in the Baculovirus/Sf9 cells system
  13. 7393
    “…Consequently, as sequence cassettes, these may be suitable for building into engineered proteins to confer small-molecule modulation on them. …”
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    SynPharm: A Guide to PHARMACOLOGY Database Tool for Designing Drug Control into Engineered Proteins
  14. 7394
    “…Therefore, construction and transformation of the gene cassettes can be easily performed. Ethanol-dependent gene expression was observed in the transformants with no significant growth retardation or inducer consumption observed in the cells cultivated under optimized conditions. …”
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    Development of an alcohol-inducible gene expression system for recombinant protein expression in Chlamydomonas reinhardtii
  15. 7395
    “…Here, we describe targeted gene integration of fluorescent marker expression cassettes into a randomly amplified polymorphic DNA (RAPD) marker region in the W chromosome of the lepidopteran model insect Bombyx mori using transcriptional activator-like effector nuclease (TALEN)–mediated genome editing. …”
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    Silkworm genetic sexing through W chromosome-linked, targeted gene integration
  16. 7396
    “…The genome level annotations provide further evidence for gene-level exchange between virus and host in these hot springs, and augments our knowledgebase for bacteriophages, archaeal viruses and Clustered Regularly Interspaced Short Palindromic Repeat cassettes, which provide a critical resource for studying viromes in extreme natural environments.…”
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    Bacterial and Archaeal Viruses of Himalayan Hot Springs at Manikaran Modulate Host Genomes
  17. 7397
    “…Our strategy utilizes synthesized sgRNA expression cassettes to direct a nuclease-null Cas9 complex fused with transcriptional activators (VP64, p65, and Rta) for site-specific induction of endogenous genes. …”
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    A Simple Cloning-free Method to Efficiently Induce Gene Expression Using CRISPR/Cas9
  18. 7398
    “…The bla(IMP–63) gene that encodes this carbapenemase turned out to be located in the variable region of four integrons (In1297, In1574, In1573, and In1572) and to coexist with novel or rare gene cassettes (fosM, gcu170, gcuF1) and insertion elements (ISPsp7v, ISPa16v). …”
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    Identification of Diverse Integron and Plasmid Structures Carrying a Novel Carbapenemase Among Pseudomonas Species
  19. 7399
    “…Particular attention will be reserved to viral vectors derived from herpes simplex type 1, a potential tool for the delivery and expression of multiple transgene cassettes simultaneously.…”
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    Gene Therapy Tools for Brain Diseases
  20. 7400
    por Ma, Ting, Li, Zhiying, Wang, Sheng
    Publicado 2019
    “…Several gene expression cassettes were designed, synthesized in vitro, and then cloned into Tobacco mosaic virus RNA-based overexpression vector. …”
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    Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System in Nicotiana benthamiana
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