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  1. 7421
    “…This antigenic variation system is driven by segmental gene conversion events that transfer information from a series of silent cassettes (vls2-16) to the expression locus, vlsE. …”
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    The Putative Endonuclease Activity of MutL Is Required for the Segmental Gene Conversion Events That Drive Antigenic Variation of the Lyme Disease Spirochete
  2. 7422
    “…Both codon optimization of the iLOV_NI01 fusion cassettes and different integration strategies into the P. pastoris genome were tested to produce and isolate a high‐yielding strain. …”
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    The fluorescent protein iLOV as a reporter for screening of high‐yield production of antimicrobial peptides in Pichia pastoris
  3. 7423
    “…Here we used a tissue culture transgenic approach for curing field cultivars of Solanum tuberosum through the stimulation of RNA interference (RNAi)-based antiviral defenses. Expression cassettes carrying inverted repeats of potato virus S (PVS, genus Carlavirus) movement or coat protein sequences were used for the transformation of potato cultivars naturally infected with PVS and/or a related carlavirus potato virus M (PVM), without or with potato virus Y (PVY, genus Potyvirus). …”
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    Virus Elimination from Naturally Infected Field Cultivars of Potato (Solanum tuberosum) by Transgenic RNA Interference
  4. 7424
    por Hillary, V. Edwin, Ceasar, S. Antony
    Publicado 2022
    “…This system incorporates fragments of foreign DNA (spacers) into CRISPR cassettes, which are further transcribed into the CRISPR arrays and then processed to make guide RNA (gRNA). …”
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    A Review on the Mechanism and Applications of CRISPR/Cas9/Cas12/Cas13/Cas14 Proteins Utilized for Genome Engineering
  5. 7425
    “…These properties were achieved by introducing the dmo and pat expression cassettes. The molecular characterisation data and bioinformatic analyses do not identify issues requiring food/feed safety assessment. …”
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    Assessment of genetically modified maize MON 87419 for food and feed uses, under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2017‐140)
  6. 7426
    “…The extracellular proteolytic activity of pseudolysin reached 258.95 U/mL with optimized expression cassettes. In addition, the S. cerevisiae expression system increased the salt tolerance of pseudolysin to sodium chloride (NaCl)and sodium dodecyl sulfate (SDS) and the recombinant pseudolysin retained 15.19% activity when stored in 3 M NaCl for 7 days. …”
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    Expression of a Salt-Tolerant Pseudolysin in Yeast for Efficient Protein Hydrolysis under High-Salt Conditions
  7. 7427
    “…This tool allows to (i) transform yeast with both Cas9 and gRNA expression cassettes in a single plasmid and (ii) insert the 20-mer sequence in the plasmid with high efficiency, thanks to Golden Gate Assembly and (iii) a red chromoprotein-based screening to speed up the selection of correct plasmids. …”
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    pCEC-red: a new vector for easier and faster CRISPR-Cas9 genome editing in Saccharomyces cerevisiae
  8. 7428
    “…AtGRF5 and AtPLT5 expression cassettes were added to CRISPR/Cas9 genome-editing vectors to obtain transgenic phytoene desaturase CmPDS knockout mutants. …”
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    Optimised Agrobacterium-Mediated Transformation and Application of Developmental Regulators Improve Regeneration Efficiency in Melons
  9. 7429
    “…IntI1 integrase is a tyrosine recombinase involved in the mobility of antibiotic resistance gene cassettes within bacterial class 1 integrons. Recent data have shown that its recombination specifically involves the bottom strand of the attC site, but the exact mechanism of the reaction is still unclear. …”
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    A New In Vitro Strand Transfer Assay for Monitoring Bacterial Class 1 Integron Recombinase IntI1 Activity
  10. 7430
    “…One of the most powerful techniques for studying the function of a gene is to disrupt the expression of that gene using genetic engineering strategies such as targeted recombination or viral integration of gene trap cassettes. The tremendous utility of these tools was recognized this year with the awarding of the Nobel Prize in Physiology or Medicine to Capecchi, Evans, and Smithies for their pioneering work in targeted recombination mutagenesis in mammals. …”
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    Unintentional miRNA Ablation Is a Risk Factor in Gene Knockout Studies: A Short Report
  11. 7431
    “…As a result, transgene expression constructs can be difficult to assemble due to the need to include two expression cassettes on a single plasmid, one to drive expression of the transgene of interest and a second for expression of the selectable marker. …”
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    A regulatable transgene expression system for cultured Plasmodium falciparum parasites
  12. 7432
    por Lynd, Amy, Lycett, Gareth John
    Publicado 2012
    “…Although there was minor variation in reporter gene activity between the different crosses examined, the tissue specific expression pattern was consistent regardless of the genomic location of the transgene cassettes. The results show that the modified Gal4-UAS system can be used to successfully activate expression of transgenes in a robust and tissue specific manner in Anopheles gambiae. …”
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    Development of the Bi-Partite Gal4-UAS System in the African Malaria Mosquito, Anopheles gambiae
  13. 7433
    “…Then cas genes were assigned to gene cassettes and to functional modules for adaptation and interference. …”
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    CRISPR adaptive immune systems of Archaea
  14. 7434
    “…Two different amplification products, which were approximately 750 and 1,700 bp in size, were obtained from amplified variable regions (in-F/in-R primers) in 3 (14.3%) and 4 (19%) of the EHEC isolates, which corresponded to dfrA7(dihydrofolate reductase type I) and dfrA1/aadA1(dihydrofolate reductase/aminoglycoside adenyltransferase) resistance gene cassettes, respectively, and this was confirmed by sequencing. …”
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    Molecular Characterization of Enterohemorrhagic Escherichia coli Isolates from Cattle
  15. 7435
    “…We used this system to insert large cassettes encoded by plasmid donors and smaller changes encoded by single-stranded oligonucleotide donors (ssODN). …”
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    CRISPR Co-Editing Strategy for Scarless Homology-Directed Genome Editing
  16. 7436
    “…PM(2.5) was collected on polycarbonate membrane filters housed in 37 mm cassettes coupled with PM(2.5) cyclones. Scanning electron microscopy coupled with energy-dispersive spectroscopy was used to study the morphology, and inductively coupled plasma-mass spectroscopy was used to analyse the elemental composition of the PM(2.5). …”
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    Physicochemical Properties of Indoor and Outdoor Particulate Matter 2.5 in Selected Residential Areas near a Ferromanganese Smelter
  17. 7437
    “…We previously developed genetically modified murine hepatoma cell lines with inducible enhanced liver functions, in which eight liver-enriched transcription factor (LETF) genes were introduced into hepatoma cells as inducible transgene expression cassettes. Here, we establish a human hepatoma cell line with heat-inducible liver functions using HepG2 cells. …”
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    HepG2-Based Designer Cells with Heat-Inducible Enhanced Liver Functions
  18. 7438
    “…According to the yields of β-nootkatol and (+)-nootkatone by strains harboring different expression cassettes in the resting cell assay, premnaspirodiene oxygenase from Hyoscyamus muticus (HPO), cytochrome P450 reductase from Arabidopsis thaliana (AtCPR) and alcohol dehydrogenase (ADH1) from Saccharomyces cerevisiae were finally selected and overexpressed in CEN·PK2-1Ca, yielding β-nootkatol and (+)-nootkatone with 170.5 and 45.6 mg L(−1) ethyl acetate, respectively. …”
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    Stepwise engineering of Saccharomyces cerevisiae to produce (+)-valencene and its related sesquiterpenes
  19. 7439
    “…Twelve unique KEGG orthologs were associated only to T(0), including tetracycline resistance cassettes. ML classified the GM at T(1) with 100% score at phylum level. …”
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    Ecology and Machine Learning-Based Classification Models of Gut Microbiota and Inflammatory Markers May Evaluate the Effects of Probiotic Supplementation in Patients Recently Recovered from COVID-19
  20. 7440
    “…In this work, we present a newly programmed automatic synthesis process of [(68)Ga]Ga-FAPI-46 on a Scintomics GRP module using two Galli Ad generators as a radionuclide source. Dedicated cassettes for the labelling of (68)Ga-peptides were used without any modifications. …”
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    Automated Synthesis of [(68)Ga]Ga-FAPI-46 on a Scintomics GRP Synthesizer
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