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  1. 7501
    “…We equipped yeast with isobutanol cassettes which had either a mitochondrial or cytosolic localized isobutanol pathway and used either a redox-imbalanced (NADPH-dependent) or redox-balanced (NADH-dependent) ketol-acid reductoisomerase enzyme. …”
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    Comparative functional genomics identifies an iron-limited bottleneck in a Saccharomyces cerevisiae strain with a cytosolic-localized isobutanol pathway
  2. 7502
    “…We highlight the critical factors required to enhance mutation induction and hairy root transformation, including the new generation of reporter genes, methods of Agrobacterium infection, accurate gRNA design strategies, Cas9 variants, gene regulatory elements of gRNAs and Cas9 nuclease cassettes and their configuration in the final binary vector to study genes involved in root-related traits in soybean.…”
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    CRISPR/Cas9 in Planta Hairy Root Transformation: A Powerful Platform for Functional Analysis of Root Traits in Soybean
  3. 7503
    “…Out of 40 IPCR amplicons, we confirmed and verified the genetic contexts of 11 samples amplified with primers targeting integron gene cassettes (GCs), IS431 composite transposons, and Tn916 conjugative transposons (tet(M) and xis-int). …”
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    Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome
  4. 7504
    “…The results demonstrate that the thermo-photonic reader in conjunction with COVID-19 IgG/IgM test cassettes can detect and quantify IgG levels in COVID-19 antibody assays within the clinically relevant range and with a high correlation to those obtained from qELISA. …”
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    Rapid and Low-Cost Detection and Quantification of SARS-CoV-2 Antibody Titers of ICU Patients with Respiratory Deterioration Using a Handheld Thermo-Photonic Device
  5. 7505
    “…Polymerase chain reaction and sequencing experiments revealed that these elements generate a circular copy of one specific strand without leaving an empty site on the donor molecule, in contrast to the movement of integron gene cassettes or ICE/IMEs discovered to date. These elements are termed SEs (strand-biased circularizing integrative elements): SE-6945 (the 7.1 kb element) and SE-6283 (Tn6283). …”
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    Atypical integrative element with strand-biased circularization activity assists interspecies antimicrobial resistance gene transfer from Vibrio alfacsensis
  6. 7506
    CD Audiom
    5 Symphonien ; Ouvertüren
  7. 7507
    por Androsiuk, Lucy, Shay, Tal, Tal, Shay
    Publicado 2023
    “…Three plasmids were identified in other public marine metagenomic data from different locations all over the world; these plasmids contained different cassettes of functional genes at each location. Analysis of antibiotic and metal resistance genes revealed that the same positions that were enriched with genes encoding resistance to antibiotics were also enriched with resistance to metals, suggesting that plasmids contribute site-dependent phenotypic modules to their ecological niches. …”
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    Characterization of the Environmental Plasmidome of the Red Sea
  8. 7508
    “…The system consists of a series of twelve Gateway Entry cassettes into which a gene of interest can be inserted using traditional cloning methods to generate either N- or C-terminal fusions to epitope tags and fluorescent proteins. …”
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    A modified Gateway cloning strategy for overexpressing tagged proteins in plants
  9. 7509
    “…CONCLUSIONS: We have worked out a chemically regulated gene expression system that can be finely tuned to produce temporally controlled “waves” in gene expression. The use of cassettes containing the CYC6 promoter, and of modified growth media, is a reliable and economically sustainable system for the temporally controlled expression of foreign genes in Chlamydomonas.…”
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    An Optimized, Chemically Regulated Gene Expression System for Chlamydomonas
  10. 7510
    “…The contribution of foreign gene expression cassettes in the F14.5L, J2R and A56R loci is analyzed, in particular the contribution of F14.5L inactivation to the reduced virulence is demonstrated by comparing the virulence of GLV-1h68 with its F14.5L-null and revertant viruses. …”
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    The highly attenuated oncolytic recombinant vaccinia virus GLV-1h68: comparative genomic features and the contribution of F14.5L inactivation
  11. 7511
    “…METHODOLOGY/PRINCIPAL FINDINGS: In the split-CreERT2 system, Cre-mediated DNA recombination is controlled by two expression cassettes as well as the time of tamoxifen application. …”
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    Split-CreERT2: Temporal Control of DNA Recombination Mediated by Split-Cre Protein Fragment Complementation
  12. 7512
    “…Nine distinct integron types were detected in 28% of the isolates belonging to 11 Salmonella serovars including S. Tallahassee. Gene cassettes identified were aadA1, aadA2, aadA5, bla (PSE-1), bla (OXA-30), dfrA1, dfrA12, dfrA17, and sat, as well as open reading frames with unknown functions. …”
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    Antimicrobial Resistance, Class 1 Integrons, and Genomic Island 1 in Salmonella Isolates from Vietnam
  13. 7513
    “…Here, we demonstrate that a nanoscale assembly line can indeed be realized by the judicious combination of three known DNA-based modules: a DNA origami2 tile that provides a framework and track for the assembly process, cassettes containing three distinct two-state DNA machines that serve as programmable cargo-donating devices3,4 and are attached4,5 in series to the tile, and a DNA walker that can move on the track from device to device and collect cargo. …”
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    A Proximity-Based Programmable DNA Nanoscale Assembly Line
  14. 7514
    “…Current molecular diagnostic techniques for susceptibility testing of septicemia rely on genotyping for the presence of known resistance cassettes. This technique is intrinsically vulnerable due to the inability to detect newly emergent resistance genes. …”
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    A Real-Time PCR Antibiogram for Drug-Resistant Sepsis
  15. 7515
    “…CONCLUSIONS: We designed and tested a modular system to rapidly clone miRNA expression cassettes. Our strategy reduces the hands-on time required to successfully generate effective constructs, and can be implemented in labs with minimal molecular cloning expertise. …”
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    Construction of permanently inducible miRNA-based expression vectors using site-specific recombinases
  16. 7516
    “…Regarding the collection of health education materials available, majority 35 (70%) had printed books followed by audio visual (AV) materials (slides, videos, audio cassettes) [22 (44%)]. 35 (70%) of participants reported that they loan only pamphlets and broachers to the public. …”
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    Role of Private-Public Partnership in Health Education: A Survey of Current Practices in Udaipur City, Rajasthan, India
  17. 7517
    “…METHODS: miRNA reporter constructs were generated using a recombinant adeno-associated virus vector, which contained complementary sequences for six miRNAs (miR-200a, miR-200b, miR-21, miR-146a, miR-155, and miR-221), along with two independent expression cassettes encoding the fluorescent reporter genes Fluc and Gluc. …”
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    The Real-Time Dynamic Monitoring of microRNA Function in Cholangiocarcinoma
  18. 7518
    “…“PAS-cal” is an artificial polypeptide concatamer of peptide cassettes with varying lengths, each composed of the three small, chemically stable amino acids Pro, Ala, and Ser, which are interspersed by Arg residues to allow site-specific cleavage with trypsin. …”
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    PAS-cal: a Generic Recombinant Peptide Calibration Standard for Mass Spectrometry
  19. 7519
    por Kaykas, Ajamete, Moon, Randall T
    Publicado 2004
    “…We have generated pHippy vectors or expression cassettes that knock down the expression of both reporter and endogenous genes. …”
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    A plasmid-based system for expressing small interfering RNA libraries in mammalian cells
  20. 7520
    “…Key steps in these syntheses include a successful double Lansbury glycosylation en route to the N-terminal fragment of β-hCG and the sequential installation of four O-linked glycosyl-amino acid cassettes into closely spaced O-glycosylation sites in a single, high-yielding solid-supported synthesis to access the C-terminal portion of the molecule. …”
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    Chemical Synthesis of the β-Subunit of Human Luteinizing (hLH) and Chorionic Gonadotropin (hCG) Glycoprotein Hormones
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