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7521“…Class 1 integrons were prevalent (28.9%) and contained previously described gene cassettes that are implicated primarily in resistance to aminoglycosides and trimethoprim (dfrA1, dfrA17-aadA5). …”
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7522“…We made publicly available through the Addgene repository a comprehensive set of MXS-building blocks comprising custom vectors, a set of fluorescent proteins, constitutive promoters, polyadenylation signals, selection cassettes and tools for inducible gene expression. …”
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7523“…The mobilome is a pool of genes located within mobile genetic elements (MGE), such as plasmids, IS elements, transposons, genomic/pathogenicity islands, and integron-associated gene cassettes. These genes are often referred to as “flexible” and may encode virulence factors, toxic compounds as well as resistance to antibiotics. …”
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7524por Upadhyay, Supriya, Hussain, Abbas, Mishra, Shweta, Maurya, Anand Prakash, Bhattacharjee, Amitabha, Joshi, Santa Ram“…Presence of integrons and gene cassettes were analyzed by integrase and 59 base-element PCR respectively. …”
Publicado 2015
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7525por Bu, Pengli, Alam, Sheikh M. Khorshed, Dhakal, Pramod, Vivian, Jay L., Soares, Michael J.“…The Prl7b1 null mutant allele was generated via replacement of the endogenous Prl7b1 coding sequence with beta-galactosidase (LacZ) reporter and neomycin cassettes. The mutant Prl7b1 allele was successfully passed through the germline. …”
Publicado 2016
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7526“…Single guide RNA (gRNA) cassettes with these target sequences were cloned into lentiviral vectors encoding the Streptococcus pyogenes Cas9 endonuclease (SpCas9) gene. …”
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7527“…However, the expression levels of important endogenous pluripotency markers, Klf4, c-myc, Rex1, and Utf1, were significantly lower in CAG-miPSC compared with TetO-miPSC with silenced reprogramming cassettes. Surprisingly, the endogenous Oct4 and Sox2 expression levels were not affected by the residual transgene expression. …”
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7528por Lampignano, Rita, Yang, Liwen, Neumann, Martin H. D., Franken, André, Fehm, Tanja, Niederacher, Dieter, Neubauer, Hans“…After enrichment, cells captured in Parsortix™ cassettes were stained in situ for nuclei, cytokeratins, EpCAM and CD45. …”
Publicado 2017
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7529“…In addition, dropout cassettes encoding chromogenic proteins were implemented for cost-free visible cloning screening that color-code different cloning levels. …”
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7530por Yang, Yang, Zhu, Kaiyu, Li, Huailin, Han, Shaoqing, Meng, Qingwei, Khan, Shahid Ullah, Fan, Chuchuan, Xie, Kabin, Zhou, Yongming“…We also assessed the efficiency of the horizontal transfer of Cas9/gRNA cassettes by pollination. Our findings reveal the potential for plant breeding strategies to improve yield traits in currently cultivated rapeseed varieties.…”
Publicado 2018
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7531por Li, Mingguang, Fine, Ryan D., Dinda, Manikarna, Bekiranov, Stefan, Smith, Jeffrey S.“…The NAD(+)-dependent histone deacetylase Sir2 was originally identified in Saccharomyces cerevisiae as a silencing factor for HML and HMR, the heterochromatic cassettes utilized as donor templates during mating-type switching. …”
Publicado 2019
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7532por Schwarzer, Philipp, Tsypik, Olga, Zuo, Chijian, Alali, Ahmad, Wunsch-Palasis, Julia, Heitzler, Tanja, Derochefort, Jana, Bernhardt, Mirjam, Yan, Xiaohui, Paululat, Thomas, Bechthold, Andreas“…A total of eight genes were deleted and six gene cassettes were generated. Rishirilide production was not strongly affected by mutations in rslO2, rslO6 and rslH. …”
Publicado 2020
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7533Evaluation of Plant-Derived Promoters for Constitutive and Tissue-Specific Gene Expression in Potatopor Miroshnichenko, Dmitry, Firsov, Aleksey, Timerbaev, Vadim, Kozlov, Oleg, Klementyeva, Anna, Shaloiko, Lyubov, Dolgov, Sergey“…In the present study, four promoters derived from the potato genome have been characterized by the expression of identical cassettes carrying the fusion with the reporter β-glucuronidase (gusA) gene. …”
Publicado 2020
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7534por Zhao, Xiaonan, Hu, Ming, Zhang, Qing, Zhao, Cui, Zhang, Yin, Li, Lulu, Qi, Jing, Luo, Yanbo, Zhou, Dong, Liu, Yuqing“…Class 1 integrons were detected in 26.9% of these isolates and contained 7 groups of resistance gene cassettes. Multilocus sequence typing analysis revealed 7 sequence types, and ST11 was the most frequent sequence types. …”
Publicado 2020
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7535“…To explore the feasibility of establishing a combined rotavirus-SARS-CoV-2 vaccine, we generated recombinant (r)SA11 rotaviruses with modified segment 7 RNAs that contained coding cassettes for NSP3, a translational 2A stop-restart signal, and a FLAG-tagged portion of the SARS-CoV-2 spike (S) protein: S1 fragment, N-terminal domain (NTD), receptor-binding domain (RBD), extended RBD (ExRBD), or S2 core (CR) domain. …”
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7536por David, Anura, Scott, Lesley, Jugwanth, Sarika, Gededzha, Maemu, Kahamba, Trish, Zwane, Nontobeko, Mampeule, Nakampe, Sanne, Ian, Stevens, Wendy, Mayne, Elizabeth S.“…Performance of the lateral flow tests were similar to sensitivities and specificities reported in other studies. The cassettes of the majority of kits evaluated (90%) detected both IgG and IgM. …”
Publicado 2021
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7537“…Moreover, these isolates harbor integrons with five different gene cassettes identified for the first time, which are associated with resistance to trimethoprim, streptomycin, tetracycline, sulfonamide, chloramphenicol, and gentamicin. …”
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7538“…The presence of these antibiotic resistance genes and cassettes were positively correlated with the presence of pathogens. …”
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7539por Alsing, Sidsel, Doktor, Thomas Koed, Askou, Anne Louise, Jensen, Emilie Grarup, Ahmadov, Ulvi, Kristensen, Lasse Sommer, Andresen, Brage Storstein, Aagaard, Lars, Corydon, Thomas J.“…To enhance efficacy and to investigate improvements in safety, we have generated VEGFA-targeting agshRNAs and microRNA (miRNA)-embedded agshRNAs (miR-agshRNAs) and inserted these RNAi effectors in Pol II/III-driven expression cassettes and lentiviral vectors (LVs). Compared with corresponding shRNAs, agshRNAs and miR-agshRNAs increased specificity and safety, while retaining a high knockdown efficacy and abolishing passenger strand activity. …”
Publicado 2022
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7540por Liu, Ke, Lin, Gui-Hong, Liu, Kun, Liu, Yong-Jun, Tao, Xin-Yi, Gao, Bei, Zhao, Ming, Wei, Dong-Zhi, Wang, Feng-Qing“…Genomic integration of genes and pathway-sized DNA cassettes is often an indispensable way to construct robust and productive microbial cell factories. …”
Publicado 2022
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