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  1. 7561
    “…For proof-of-principle studies, we constructed multiplex siRNA vectors silencing β-catenin and/or Smad4 and assessed their functionalities in mesenchymal stem cells (MSCs). Pooled siRNA cassettes were effectively inserted into respective entry vectors in one-step, and shotgun seamless assembly of pooled BstXI-digested SiEU fragments into a retroviral destination vector followed. …”
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    Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells
  2. 7562
    “…The sul3-type III class 1 integron (5′CS-sat-psp-aadA2-cmlA1-aadA1-qacH-IS440-sul3) and gene cassettes aac(6′)-Ib-cr-bla(OXA–)(1)-catB3-arr-3 are found in SGI1-PmSC1111. …”
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    IS26-Mediated Genetic Rearrangements in Salmonella Genomic Island 1 of Proteus mirabilis
  3. 7563
    “…Class I integrons were found in 27.3% (18/66) of the isolates and five of these integrons contained different gene cassettes (aacA4C-arr-3-dfr2, dfrA12-aadA21, aadA2, dfrA12-aadA2, dfr17-aadA5). …”
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    Incidence and Characterization of Salmonella Isolates From Raw Meat Products Sold at Small Markets in Hubei Province, China
  4. 7564
    “…Additionally, enrichment of BRD4 or MED1 at the H3K27ac enriched DMRs functioned as super-enhancers (SE), controlling the overexpression of genes or gene-cassettes. CONCLUSIONS: Therefore, this study presents the underlying epigenetic regulatory networks of gene expression dysregulation in NDMM patients and identifies potential targets for future therapies.…”
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    The functional epigenetic landscape of aberrant gene expression in molecular subgroups of newly diagnosed multiple myeloma
  5. 7565
    “…Here, we developed expression cassettes for stable integration of the rhamnolipid biosynthesis genes into the genome outperformed plasmid-based expression systems. …”
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    Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida
  6. 7566
    “…Molecular analysis of global A. baumannii isolates identified multiple AbGRI4 RI variants in non-ST2 clonal lineages, including variations in the resistance gene cassettes, integron backbone and insertion breakpoints at the hydrolase gene. …”
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    AbGRI4, a novel antibiotic resistance island in multiply antibiotic-resistant Acinetobacter baumannii clinical isolates
  7. 7567
    “…Transgenic plants generated by introduction of the rGA733-2 and rGA733-Fc expression cassettes also significantly accumulated corresponding recombinant proteins. …”
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    Optimization of the human colorectal carcinoma antigen GA733-2 production in tobacco plants
  8. 7568
    “…This strain presents two plasmids carrying MDR cassettes, pBH100, and pAp, with conjugative and mobilization properties, respectively. …”
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    Genomic Characterization of Multidrug-Resistant Escherichia coli BH100 Sub-strains
  9. 7569
    “…The tree shape and chronology allowed us to infer that the hospital-associated ST1-SCCmecIV from Brazil and the community-acquired USA400 from North America are not closely related and that they might have originated from different MSSA strains that independently acquired SCCmecIV cassettes. As expected, representatives of ST1 strains from Brazil showed lower cytotoxicity and a greater ability to survive inside human host cells. …”
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    Reductive evolution of virulence repertoire to drive the divergence between community- and hospital-associated methicillin-resistant Staphylococcus aureus of the ST1 lineage
  10. 7570
    “…All cognate V(H) and V(L) transcripts were formatted into transcription and translation compatible linear DNA expression cassettes (LEC) encoding whole IgG or Fab. Between 92% and 100% of paired V(H) and V(L) transcripts could be converted to LECs, and nearly 100% of them expressed as antibodies when transfected into Expi293F cells. …”
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    A platform-agnostic, function first-based antibody discovery strategy using plasmid-free mammalian expression of antibodies
  11. 7571
    “…The Forkhead transcription factor Fkh1 is mainly a negative transcriptional regulator which affects cell cycle control, silencing of mating-type cassettes and induction of pseudohyphal growth in the yeast Saccharomyces cerevisiae. …”
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    Forkhead transcription factor Fkh1: insights into functional regulatory domains crucial for recruitment of Sin3 histone deacetylase complex
  12. 7572
    “…It is equipped with dual gene cassettes driven by a strong inducible promoter that encode the therapeutic protein P8 fused to a secretion signal peptide and a complementation system. …”
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    A synthetic probiotic engineered for colorectal cancer therapy modulates gut microbiota
  13. 7573
    “…The qnrB gene, the variant aac(6′)-Ib-cr, and the class 1 integrons with different gene cassettes, were detected amongst our 20 isolated strains, which were classified as ExPEC and aEPEC. …”
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    First Detection of Human ST131-CTX-M-15-O25-B2 Clone and High-Risk Clonal Lineages of ESBL/pAmpC-Producing E. coli Isolates from Diarrheic Poultry in Tunisia
  14. 7574
    “…There were a diversity of class 1 integron and variations in the gene cassettes associated to bla(VIM–)(2), as well as a possible event of spread of bla(KPC–)(2) mediated by a plasmid that contained part of Tn4401b in one infection case. …”
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    Genetic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates Carrying bla(VIM–2) and bla(KPC–2) Genes That Spread on Different Genetic Environment in Colombia
  15. 7575
    “…In TprK, amino acid diversity is confined to seven variable (V) regions, and generation of sequence diversity within the V regions occurs via a non-reciprocal segmental gene conversion mechanism where donor cassettes recombine into the tprK expression site. …”
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    Longitudinal TprK profiling of in vivo and in vitro-propagated Treponema pallidum subsp. pallidum reveals accumulation of antigenic variants in absence of immune pressure
  16. 7576
    “…The vector used to produce the desired oil profile in DHA canola contains the expression cassettes of seven genes in the DHA biosynthesis pathway and was specifically designed to convert oleic acid to DHA in canola seed. …”
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    Food and Feed Safety of NS-B5ØØ27-4 Omega-3 Canola (Brassica napus): A New Source of Long-Chain Omega-3 Fatty Acids
  17. 7577
    “…We hypothesize that the extraordinary toughness of C. darwini major ampullate dragline silk may relate to the unique protein composition of major ampullate spidroins, combined with the relatively high expression of stretchy flagelliform spidroins whose union into a single fiber may be aided by novel motifs and cassettes that act as molecule-binding helices. Our assemblies extend the catalog of sequences and sets of expressed genes that confer the unique biophysical properties observed in natural silks.…”
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    Characterization of the genome and silk-gland transcriptomes of Darwin’s bark spider (Caerostris darwini)
  18. 7578
    “…Collagenase secretion was increased by optimization of expression cassettes. Collagenase expression imposed metabolic burden and cofactor perturbations on yeast cells, which could be improved through metabolic engineering. …”
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    Secretion of collagenases by Saccharomyces cerevisiae for collagen degradation
  19. 7579
    “…In addition to confirming the known canonical viral early and late RNA cassettes, our analysis of splice junctions within long RNA reads revealed an additional 35 novel viral transcripts that meet stringent criteria for expression. …”
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    Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts
  20. 7580
    “…DHO-expressing strains were highly sensitive to 5-fluorodihydrouracil (5F-dhu) and plasmids bearing expression cassettes for DHO were readily lost during growth on 5F-dhu-containing media. …”
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    Identification of fungal dihydrouracil-oxidase genes by expression in Saccharomyces cerevisiae
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