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  1. 7601
    “…These bacteria normally acquire their antibiotic resistance via several different mobile genetic elements including plasmids, transposons, and integrons involving gene cassettes. During a diarrhea surveillance study in Thimphu, Bhutan in June and July, 2011, Shigella sonnei were isolated more frequently than expected. …”
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    Molecular characterization and PCR-based replicon typing of multidrug resistant Shigella sonnei isolates from an outbreak in Thimphu, Bhutan
  2. 7602
    “…In total, 33 DEGs related to transport processes were found, including 12 proton pump genes, three ATP-binding cassettes (ABCs), 13 solute carrier (SLC) family members, two sweet sugar transporter (ST) family members and three other substance transporters. …”
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    Transcriptome Changes in Eriocheir sinensis Megalopae after Desalination Provide Insights into Osmoregulation and Stress Adaption in Larvae
  3. 7603
    “…We used SPI to simultaneously insert up to four different gene cassettes in a single recombineering reaction. Consequently, our findings have important implications for the understanding of E. coli replication and Red recombination.…”
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    Lambda Red Mediated Gap Repair Utilizes a Novel Replicative Intermediate in Escherichia coli
  4. 7604
    por Flórez, Ana Belén, Mayo, Baltasar
    Publicado 2015
    “…Sequence analysis identified eight complete open reading frames (orfs) in pLG9 and 36 in pLG42; these were organized into functional modules or cassettes containing different numbers of genes. These modules were flanked by complete or interrupted insertion sequence (IS)-like elements. …”
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    The Plasmid Complement of the Cheese Isolate Lactococcus garvieae IPLA 31405 Revealed Adaptation to the Dairy Environment
  5. 7605
    “…In the current study, we designed a panel of HSV-1 mutants, in which portions of UL13 and US3 genes were replaced by expression cassettes encoding mCherry protein or green fluorescent protein (GFP), respectively, and analyzed DNA replication, protein expression, and spread of these mutants in several cell types. …”
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    The UL13 and US3 Protein Kinases of Herpes Simplex Virus 1 Cooperate to Promote the Assembly and Release of Mature, Infectious Virions
  6. 7606
    “…The strategy was three-pronged: (1) once there was confidence diagnostic tissue had been obtained and if safe for the patient, additional biopsy passes were performed to further increase volume of biopsy material, (2) biopsy material was placed in multiple cassettes for processing, and (3) all tissue ribbons were conserved when cutting blocks in the histology laboratory. …”
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    Effects of a Multidisciplinary Approach to Improve Volume of Diagnostic Material in CT-Guided Lung Biopsies
  7. 7607
    “…The tissue-specific polycistronic system used in the present study consisted of two expression cassettes, separated by an insulator: (i) 11-β-hydroxysteroid dehydrogenase 1 (11β-HSD1), driven by the porcine liver-specific apolipoprotein E promoter; (ii) human islet amyloid polypeptide (hIAPP) and C/EBP homologous protein (CHOP), linked to the furin digested site and F-2A, driven by the porcine pancreas-specific insulin promoter. …”
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    Multi-transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis
  8. 7608
    “…It is shown that the DEBS genes are functionally expressed in B. subtilis when the native eryAI–III operon was separated into three individual expression cassettes with optimized ribosomal binding sites. A synthesis of 6dEB could be detected by using the acetoin-inducible acoA promoter and a fed-batch simulating EnBase-cultivation strategy. …”
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    Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis
  9. 7609
    “…Three of these harbor extensive multi-drug resistance cassettes encoding resistance against the majority of drugs used to combat infection in beef cattle, including macrolides and tetracyclines used in human medicine. …”
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    Comparative Genomic Analysis of Mannheimia haemolytica from Bovine Sources
  10. 7610
    “…There were no issues and failures with the system or the synthesis cassettes. The average radiochemical purity as determined by ITLC was above 99% (99.8 ± 0.05%) and the average radiochemical purity as determined by HPLC technique was above 97% (97.3 ± 1.5%) for this period. …”
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    Lutetium-177 DOTATATE Production with an Automated Radiopharmaceutical Synthesis System
  11. 7611
    “…In addition, the test cassettes can be used as a vehicle to ship viral RNA to reference laboratories for further laboratory confirmation of the diagnosis and for epidemiological investigations using nucleotide sequencing. …”
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    Validation of a Rapid Rabies Diagnostic Tool for Field Surveillance in Developing Countries
  12. 7612
    “…To achieve this, genes related to the metabolic pathway were maximally expressed to amplify the flux toward cadaverine production via synthetic expression cassettes consisting of predictive and quantitative genetic parts (promoters, 5′-untranslated regions, and terminators). …”
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    Synthetic redesign of Escherichia coli for cadaverine production from galactose
  13. 7613
    “…Transcriptome analyses using this enriched oligonucleotide platform identified differential shifts in the response to PGN and LPS in macrophage-like cells, highlighting responsive gene-cassettes tightly related to PAMP host recognition. …”
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    Extending Immunological Profiling in the Gilthead Sea Bream, Sparus aurata, by Enriched cDNA Library Analysis, Microarray Design and Initial Studies upon the Inflammatory Response to PAMPs
  14. 7614
    “…Dominant selectable cassettes were introduced into each plasmid, allowing for selection following gene transfer into A. fumigatus by incorporating phleomycin or hygromycin into the medium. …”
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    Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus
  15. 7615
    “…Class 1 integrons were detected in 16.9% (26/154) of these isolates and contained gene cassettes aadA2, aadA1, drfA1-aadA1, drfA12-aadA2, and drfA17-aadA5. …”
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    Characterization of Integrons and Resistance Genes in Salmonella Isolates from Farm Animals in Shandong Province, China
  16. 7616
    “…The dph3-ATGmut and a dph3::loxP-ura4-loxM gene disruption strain, but not msh3-ATGmut, turned out to be sensitive to hydroxyurea and methyl methanesulfonate, likewise the strains with cassettes integrated at the msh3 locus. The fungicide sordarin, which inhibits diphthamide modified eEF2 of Saccharomyces cerevisiae, barely affected survival of wild type and msh3Δ S. pombe cells, while the dph3Δ mutant was sensitive. …”
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    A mutated dph3 gene causes sensitivity of Schizosaccharomyces pombe cells to cytotoxic agents
  17. 7617
    “…The identification of Sr13 expands the number of Pgt-resistance genes that can be incorporated into multigene transgenic cassettes to control this devastating disease.…”
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    Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group
  18. 7618
    “…Lx-shRNA157-1694 (an shRNA expression plasmid containing two shRNA expression cassettes) and mouse immortal (mi)MSCs stably expressing shRNA (miMSC-shRNA) were constructed and their suppressive effects on HBV expression were investigated using reverse transcription-polymerase chain reaction (RT-PCR), ELISA and immunofluorescence. …”
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    Therapeutic effects of mesenchymal stem cells combined with short hairpin RNA on liver injury induced by hepatitis B virus infection
  19. 7619
    “…The structure of class 1 integrons and their gene cassettes was also analyzed. Insertion sequence IS26 was present upstream of bla(TEM-1), ISEcp1 was present upstream of bla(CTXM-15) gene and orf477 was present downstream of bla(CTXM-15). …”
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    Genetic Environment of bla(TEM-1), bla(CTX-M-15), bla(CMY-42) and Characterization of Integrons of Escherichia coli Isolated From an Indian Urban Aquatic Environment
  20. 7620
    “…Our study is the first to demonstrate successful in vitro conjugation, which indicated that XDR-AB contained combination mechanisms of the co-transfer of antimicrobial resistance elements with integron cassettes or with the plasmid group GR6. Thus, conjugation could be responsible for the emergence of new types of antibiotic-resistant strains.…”
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    Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii
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