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7741por von Jonquieres, Georg, Fröhlich, Dominik, Klugmann, Claudia B., Wen, Xin, Harasta, Anne E., Ramkumar, Roshini, Spencer, Ziggy H. T., Housley, Gary D., Klugmann, Matthias“…Recombinant AAV expression cassettes carrying the cDNA encoding enhanced green fluorescent protein (GFP) driven by truncated versions of the recombinant MAG promoter (2.2, 1.5 and 0.3 kb in size) were packaged as cy5 vectors and delivered into the dorsal striatum of mice. …”
Publicado 2016
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7742por Evans, Sue M., Patabendi Bandarage, Varuni, Kronborg, Caroline, Earnest, Arul, Millar, Jeremy, Clouston, David“…Patients who were diagnosed by transperineal biopsy compared to transrectal ultrasound (OR = 0.6, 95% CI = 0.5–0.8) and higher percentage of positive biopsy cassettes (25–62.5%: OR = 0.7, 95% CI = 0.6–0.8, > 62.5: OR = 0.6, 95% CI = 0.5–0.8) were significantly associated with less likelihood of upgrade. …”
Publicado 2016
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7743por Peng, Bingyin, Plan, Manuel R., Carpenter, Alexander, Nielsen, Lars K., Vickers, Claudia E.“…However, repeated use of the CUP1 promoter in multiple expression cassettes resulted in genetic instability. A diauxie-inducible expression system, based on an engineered GAL regulatory circuit and a set of four different GAL promoters, was characterized and employed to assemble nerolidol synthetic metabolic modules. …”
Publicado 2017
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7744“…However, the current state of the technology relies heavily on DNA-based expression cassettes for delivering Cas9 and the guide RNA (gRNA) to the cell. …”
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7745por Cummins, Max L., Reid, Cameron J., Roy Chowdhury, Piklu, Bushell, Rhys N., Esbert, Nicolas, Tivendale, Kelly A., Noormohammadi, Amir H., Islam, Shaiful, Marenda, Marc S., Browning, Glenn F., Markham, Philip F., Djordjevic, Steven P.“…Notably, mobile resistance genes conferring resistance to fluoroquinolones, colistin, extended-spectrum β-lactams and carbapenems were not detected in the genomes of these 95 APEC but carriage of the sulphonamide resistance gene, sul1 (59/95, 63 %), the trimethoprim resistance gene cassettes dfrA5 (48/95, 50 %) and dfrA1 (25/95, 27 %), the tetracycline resistance determinant tet(A) (51/95, 55 %) and the ampicillin resistance genes bla(TEM-1A/B/C) (48/95, 52 %) was common. …”
Publicado 2019
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7746“…Those who knew their HBV and HCV status and those who did not know their status were tested for circulating HBsAg and anti-HCV using rapid diagnostic test cassettes. Descriptive analysis was done, and the data presented as median with interquartile range, frequency and percentage. …”
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7747por Ramamurthy, Thandavarayan, Mutreja, Ankur, Weill, François-Xavier, Das, Bhabatosh, Ghosh, Amit, Nair, Gopinath Balakrish“…Presence of integrative conjugative elements (ICE) with antibiotic resistance gene cassettes, clustered regularly interspaced short palindromic repeats-associated protein system and ctxAB promoter based ToxRS expression of cholera toxin (CT) separates classical and El Tor biotypes. …”
Publicado 2019
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7748“…The mechanisms of control by GlnR of several promoters of ATP binding cassettes (ABC) sugar transporters and carbon metabolism are highly elaborated. …”
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7749por Jiang, Han, Yu, Ting, Yang, Yuting, Yu, Shengtao, Wu, Jiangchun, Lin, Rumeng, Li, Yixian, Fang, Jiehong, Zhu, Cheng“…However, a low percentage of class 1 integrons without gene cassettes and no class 2 or 3 integrons were detected in the 18 MDR and MHMR isolates or in the 90 V. parahaemolyticus isolates overall. …”
Publicado 2020
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7750por Wagner, Michael, Doverfjord, Johan G., Tillner, Joachim, Antoni, Gunnar, Haack, Torsten, Bossart, Martin, Laitinen, Iina, Johansson, Lars, Pierrou, Stefan, Eriksson, Olof, Velikyan, Irina“…Methods: The automated synthesis platform (Modular-Lab PharmTrace, Eckert & Ziegler, Eurotope, Germany), disposable cassettes for (68)Ga-labeling, and pharmaceutical-grade (68)Ge/(68)Ga generator (GalliaPharm(®)) used in the study were purchased from Eckert & Ziegler. …”
Publicado 2020
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7751“…ECP.81 and ECP.82 from sick pigs harboring mcr-1.1 and mcr-3.1 were serotype O9:H9 with ST10. Two mcr-1.1 gene cassettes from ECP.81 and ECP.82 were located on IncI2 plasmid with 98% identity to plasmid pHNSHP45. …”
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7752“…Furthermore, we predicted 76 potential virulence factors without homology to human proteins, including two riboflavin synthase proteins, three ATP-binding cassettes (ABC) transporter protein family proteins, and 12 outer membrane proteins (OMPs). …”
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7753por Qiao, Jie, Ge, Haoyu, Xu, Hao, Guo, Xiaobing, Liu, Ruishan, Li, Chenyu, Chen, Ruyan, Zheng, Beiwen, Gou, Jianjun“…The integron of bla (IMP-4) is In823, which is the array of gene cassettes of 5′CS-bla (IMP-4). Phylogenetic analysis demonstrated that E. hormaechei YQ13422hy and YQ13530hy belonged to the same small clusters with a high degree of homology. …”
Publicado 2022
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7754por Zhou, Jun, Yang, Chuishun, Lei, Wenjuan, Xu, Man, Cai, Xingli, Yuan, Wanqiong, Lin, Hua“…The presence of the psm-mec signal in the spectra of the MRCNS tested here demonstrates the presence of certain SCCmec cassettes that convey methicillin resistance. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03017-2.…”
Publicado 2023
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7755por Wörmann, Mirka E, Horien, Corey L, Bennett, Julia S, Jolley, Keith A, Maiden, Martin C J, Tang, Christoph M, Aho, Ellen L, Exley, Rachel M“…We also identified pilS cassettes in all isolates with class II pilE, however, our analysis indicates that these do not serve as donor sequences for pilE/pilS recombination. …”
Publicado 2014
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7756por Warner, Wayne A., Kuang, Shan N., Hernandez, Rina, Chong, Melissa C., Ewing, Peter J., Fleischer, Jen, Meng, Jia, Chu, Sheena, Terashita, Dawn, English, L’Tanya, Chen, Wangxue, Xu, H. Howard“…Class 1 integron-associated resistance gene cassettes appear to contribute to resistance to aminoglycoside antibiotics. …”
Publicado 2016
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7757por Camattari, Andrea, Goh, Amelia, Yip, Lian Yee, Tan, Andy Hee Meng, Ng, Sze Wai, Tran, Anthony, Liu, Gaowen, Liachko, Ivan, Dunham, Maitreya J., Rancati, Giulia“…Although the stability of integrated expression cassettes is well appreciated for most applications, the availability of reliable episomal vectors for this host would represent a useful tool to expedite cloning and high-throughput screening, ameliorating also the relatively high clonal variability reported in transformants from integrative vectors caused by off-target integration in the P. pastoris genome. …”
Publicado 2016
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7758por Zeng, Wen-Bo, Jiang, Hai-Fei, Gang, Ya-Dong, Song, Yi-Ge, Shen, Zhang-Zhou, Yang, Hong, Dong, Xiao, Tian, Yong-Lu, Ni, Rong-Jun, Liu, Yaping, Tang, Na, Li, Xinyan, Jiang, Xuan, Gao, Ding, Androulakis, Michelle, He, Xiao-Bin, Xia, Hui-Min, Ming, Ying-Zi, Lu, Youming, Zhou, Jiang-Ning, Zhang, Chen, Xia, Xue-Shan, Shu, Yousheng, Zeng, Shao-Qun, Xu, Fuqiang, Zhao, Fei, Luo, Min-Hua“…Among them, H129-G4 was obtained by inserting binary tandemly connected GFP cassettes into the H129 genome, and H129-ΔTK-tdT was obtained by deleting the thymidine kinase (TK) gene and adding tdTomato coding gene to the H129 genome. …”
Publicado 2017
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7759por Vazquez-Rosas, Guillermo Jose, Merida-Vieyra, Jocelin, Aparicio-Ozores, Gerardo, Lara-Hernandez, Antonino, De Colsa, Agustin, Aquino-Andrade, Alejandra“…The isolates were classified into methicillin-resistant S. aureus (MRSA) or methicillin-sensitive S. aureus (MSSA). Staphylococcal cassettes chromosome mec (SCCmec) were detected. Virulence genes (cna, clfA, clfB, eta, etb, fnbA, fnbB, hla, pvl, sec, and tsst) were amplified, and their clonal relationships were established by pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and clonal complex (CC) typing. …”
Publicado 2021
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7760por Bahariah, Bohari, Masani, Mat Yunus Abdul, Fizree, Md Piji Mohd Al Akmarul, Rasid, Omar Abd, Parveez, Ghulam Kadir Ahmad“…Using robust plant CRISPR/Cas9 vector technology, multiple sgRNA expression cassettes were efficiently constructed into a single-binary CRISPR/Cas9 vector to edit the EgFAD2 and EgPAT genes. …”
Publicado 2023
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