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7761por Salgueiro, Vanessa, Manageiro, Vera, Bandarra, Narcisa M., Ferreira, Eugénia, Clemente, Lurdes, Caniça, Manuela“…All strains showed genes encoding efflux pumps from major facilitator superfamily (e.g., arlR, lmrS-type and norA/B-type), ATP-binding cassettes (ABC; mgrA) and multidrug and toxic compound extrusion (MATE; mepA/R-type) families, all associated to decreased susceptibility to antibiotics/disinfectants. …”
Publicado 2023
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7762por Yuan, Guangsheng, Zeng, Cheng, Shi, Haoya, Yang, Yong, Du, Juan, Zou, Chaoying, Ma, Langlang, Pan, Guangtang, Shen, Yaou“…In this work, two basic transgenic maize, named KEY, carrying the Cre gene, and LOCK, containing the Vip3A gene with a blocked element, were obtained based on their separate fusion gene cassettes. The expression level and concentration of Vip3A were observed with a high specific accumulation in the green tissues (leaf and stem), and only a small amount was observed in the root and kernel tissues in the KEY × LOCK hybrids. …”
Publicado 2023
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7763por Calderon Toledo, Carla, von Mentzer, Astrid, Agramont, Jorge, Thorell, Kaisa, Zhou, Yingshun, Szabó, Miklós, Colque, Patricia, Kuhn, Inger, Gutiérrez-Cortez, Sergio, Joffré, Enrique“…Clinical strains were more often multidrug-resistant (MDR) than environmental isolates and harbored the class 1 integron-integrase gene intI1 next to the MDR cassettes. Retrospective analysis of antibiotic resistance in ETEC revealed a high frequency of MDR in clinical isolates. …”
Publicado 2023
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7764“…RESULTS: The bispecific XHR lentiviral vector harboring CXCR4 and CCR5 shRNA expression cassettes was efficient in transducing CD34+ cells. …”
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7765“…Moreover, we established CD4(+) C8166 T cell lines that stably express either R88-A3G or HA-A3G by transduction with VSV-G-pseudotyped lentiviral vector that harbor expression cassettes for R88-A3G or HA-A3G, respectively, and tested their susceptibility to Vif(+) HIV-1 infection. …”
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7766“…In a broader context, the TAS belong to the resistome domain of the prokaryotic mobilome which includes partially selfish, addictive gene cassettes involved in various aspects of stress response and organized under the same general principles as the TAS. …”
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7767por Madsen, Anne Mette, Schlünssen, Vivi, Olsen, Tina, Sigsgaard, Torben, Avci, Hediye“…PM(1) was sampled using Triplex cyclones at a working area at 14 Danish biofuel plants. Millipore cassettes were used to sample ‘total dust’. The PM(1) particles (29 samples) were analysed for content of 11 different components and the total dust was analysed for cultivable fungi, N-acetyl-β-D-glucosaminidase (NAGase), and (1 → 3)-β-D-glucans. …”
Publicado 2009
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7768por Leung, Alina A., Lou, Jerry J., Mareninov, Sergey, Silver, Steven S., Routbort, Mark J., Riben, Michael, Andrechak, Gary, Yong, William H.“…METHODS: The RFID tags were affixed to cryovials containing tissue or media, glass slides, and tissue cassettes. The tags were interrogated for readability before and after each testing condition or cycle. …”
Publicado 2010
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7769por Johnson, Jarrod S., Gentzsch, Martina, Zhang, Liqun, Ribeiro, Carla M. P., Kantor, Boris, Kafri, Tal, Pickles, Raymond J., Samulski, R. Jude“…The presence of misfolded proteins did not affect cell surface attachment of virus or influence expression levels from promoter transgene cassettes in plasmid transfection studies, indicating exploitation occurs at the level of virion trafficking or processing. …”
Publicado 2011
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7770por Mukadi, Pierre, Gillet, Philippe, Barbé, Barbara, Luamba, Jean, Lukuka, Albert, Likwela, Joris, Mumba, Dieudonné, Muyembe, Jean-Jacques, Lutumba, Pascal, Jacobs, Jan“…METHODS: The EQA consisted of (i) 10 high-resolution printed photographs displaying cassettes with real-life results and multiple choice questions (MCQ) addressing individual health workers (HW), and (ii) a questionnaire on RDT use addressing the laboratory of health facilities (HF). …”
Publicado 2015
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7771por Kartsev, Nikolay N., Fursova, Nadezhda K., Pachkunov, Dmitry M., Bannov, Vasiliy A., Eruslanov, Boris V., Svetoch, Edward A., Dyatlov, Ivan A.“…There are bla (TEM) (n = 10), bla (CTX-M-15) (n = 1), class 1 integron (n = 3) carrying resistance cassettes to aminoglycosides and sulphonamides dfrA17-aadA5 and dfrA12-orfF-aadA2. …”
Publicado 2015
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7772“…Sequencing of these amplicons revealed the presence of gene cassettes harboring aac(3)-Id, aadA2, aadA4, aadA7, sat, dfrA15, lnuF and estX resistance genes. …”
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7773por Berhane, Araia, Russom, Mulugeta, Bahta, Iyassu, Hagos, Filmon, Ghirmai, Michael, Uqubay, Selam“…During the time frame of 15 July 2015 to 19 January 2016, 65 microscopically confirmed patients were tested with Malaria RDTs SD Bioline Pf/Pv/Mixed Combo cassettes. RESULTS: A total of 65 blood specimens (50 P. falciparum, 13 Plasmodium vivax and 2 mixed) confirmed microscopically were tested against the available lots of malaria RDTs. …”
Publicado 2017
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7774“…However, the construction of gene deletions and replacements in Pseudomonas aeruginosa is relatively time-consuming, requiring multiple steps including suicide vector construction, conjugation, inactivation with insertion of antibiotic resistance cassettes and allelic exchange. Even employing Gateway recombineering techniques with direct transformation requires a minimum two weeks. …”
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7775por Liu, Xiaofeng, Wang, Min, Qin, Yufeng, Shi, Xuan, Cong, Peiqing, Chen, Yaosheng, He, Zuyong“…We compared and contrasted our method to a previously reported technique for the direction-independent integration of DNA cassettes into the genomes of cultured cells via NHEJ. …”
Publicado 2018
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7776por Priddy, Frances H, Lewis, David J M, Gelderblom, Huub C, Hassanin, Hana, Streatfield, Claire, LaBranche, Celia, Hare, Jonathan, Cox, Josephine H, Dally, Len, Bendel, Daryl, Montefiori, David, Sayeed, Eddy, Ackland, Jim, Gilmour, Jill, Schnepp, Bruce C, Wright, J Fraser, Johnson, Philip“…INTERPRETATION: Future studies should explore higher doses of AAV, alternative AAV serotypes and gene expression cassettes, or other broadly neutralising HIV antibodies. …”
Publicado 2019
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7777por Marathe, Nachiket P., Berglund, Fanny, Razavi, Mohammad, Pal, Chandan, Dröge, Johannes, Samant, Sharvari, Kristiansson, Erik, Larsson, D. G. Joakim“…High throughput sequencing of amplified integron gene cassettes identified a novel functional variant of an IMP-type (proposed IMP-81) carbapenemase gene (eight aa substitutions) along with recently described novel resistance genes like sul4 and bla(RSA1). …”
Publicado 2019
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7778“…Taking advantage of four resistance cassettes the procedure did not require recombination events between each round of integration. …”
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7779“…Genes mediating resistance to β-lactams (including bla(TEM-1), bla(CTX-M), bla(NDM), bla(IMP), bla(VIM), and bla(OXA-48/181)), fluoroquinolones (oqxAB, qnrA/B/D/S, gyrA/B, and parCE mutations, etc.), aminoglycosides (including armA and rmtC/F), sulfonamides (sul1/2/3), trimethoprim (dfrA), tetracycline [tet(A/B/C/D/G/O/M/39)], colistin (mcr-1), phenicols (catA/B, cmlA), and fosfomycin (fosA) were mostly found in Enterobacter spp. and Klebsiella pneumoniae, and also in Serratia marcescens, Escherichia coli, Salmonella enterica, Pseudomonas, Acinetobacter baumannii, etc., on mostly IncF-type, IncX(3/4), ColRNAI, and IncR plasmids, within IntI1 gene cassettes, insertion sequences, and transposons. Clonal and multiclonal outbreaks and dissemination of resistance genes across species and countries and between humans, animals, plants, and the environment were observed; Escherichia coli ST103, K. pneumoniae ST101, S. enterica ST1/2, and Vibrio cholerae ST69/515 were common strains. …”
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7780por Jørgensen, Rikke Bramming, Kero, Ida Teresia, Blom, Aleksander, Grove, Esten Eide, Svendsen, Kristin von Hirsch“…Respirable particle concentration and exposure were determined using a sampling train consisting of a pump, filter, filter cassettes, and SKC Cyclone for the respirable fraction. …”
Publicado 2020
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