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  1. 7781
    “…In this study, Kalanchoë blossfeldiana ‘Molly’ was transformed with individual rol genes and selected ORFs in 35S overexpressing cassettes to comprehensively characterize growth traits, gene copy and expression, and ethylene tolerance of the flowers. …”
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    Transgenic Kalanchoë blossfeldiana, Containing Individual rol Genes and Open Reading Frames Under 35S Promoter, Exhibit Compact Habit, Reduced Plant Growth, and Altered Ethylene Tolerance in Flowers
  2. 7782
    “…In our plasmids, mcr-9 was located in a ∼30-kb region lacking different genetic elements of the typical core structure of mcr-9 cassettes. In this region were also identified different genes involved in heavy metal metabolism. …”
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    Emergence of IncHI2 Plasmids With Mobilized Colistin Resistance (mcr)-9 Gene in ESBL-Producing, Multidrug-Resistant Salmonella Typhimurium and Its Monophasic Variant ST34 From Food-Producing Animals in Italy
  3. 7783
    “…Here, we report the important role of IS26 in forming multiple types of bla(NDM) multidrug resistance cassettes and the dynamic recombination of plasmids bearing bla(NDM). …”
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    IS26 Is Responsible for the Evolution and Transmission of bla(NDM)-Harboring Plasmids in Escherichia coli of Poultry Origin in China
  4. 7784
    “…A universal BeYDV-based vector (pGMV-U) was assembled to produce all CRISPR/Cas9 components with up to four independent guide RNA (gRNA) expression cassettes. With a focus on fungal tolerance, we used gRNA pairs to address considerably large deletions of putative grape susceptibility genes, including AUXIN INDUCED IN ROOT CULTURE 12 (VviAIR12), SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTER 4 (VviSWEET4), LESION INITIATION 2 (VviLIN2), and DIMERIZATION PARTNER-E2F-LIKE 1 (VviDEL1). …”
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    CRISPR/Cas9 Targeted Editing of Genes Associated With Fungal Susceptibility in Vitis vinifera L. cv. Thompson Seedless Using Geminivirus-Derived Replicons
  5. 7785
    por Shi, De-Li
    Publicado 2022
    “…This approach introduces several tandem guide RNA expression cassettes and a GFP reporter into transgenic embryos expressing Cas9 to create biallelic mutations and inactivate genes of interest specifically in the developing oocytes. …”
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    Circumventing Zygotic Lethality to Generate Maternal Mutants in Zebrafish
  6. 7786
    “…We used a rapid and efficient gene deletion approach based on PCR-amplified split-marker DNA cassettes together with in vitro assembled Cas9-guide RNA ribonucleoproteins (Cas9 RNPs) to generate C. cinerea cre1 gene deletion strains. …”
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    Preassembled Cas9 Ribonucleoprotein-Mediated Gene Deletion Identifies the Carbon Catabolite Repressor and Its Target Genes in Coprinopsis cinerea
  7. 7787
    por Chang, Perng-Kuang
    Publicado 2023
    “…Expression of single guide RNA (sgRNA) cassettes was controlled by the A. flavus U6 promoter and terminator. …”
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    A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section Flavi Species, Aspergillus nidulans, Aspergillus fumigatus, Aspergillus terreus, and Aspergillus niger
  8. 7788
    “…No matching ESBL plasmids or cassettes were detected between clinical Shigella and Salmonella isolates. …”
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    Using a combination of short- and long-read sequencing to investigate the diversity in plasmid- and chromosomally encoded extended-spectrum beta-lactamases (ESBLs) in clinical Shigella and Salmonella isolates in Belgium
  9. 7789
    por Imanishi, Tadashi, Itoh, Takeshi, Suzuki, Yutaka, O'Donovan, Claire, Fukuchi, Satoshi, Koyanagi, Kanako O, Barrero, Roberto A, Tamura, Takuro, Yamaguchi-Kabata, Yumi, Tanino, Motohiko, Yura, Kei, Miyazaki, Satoru, Ikeo, Kazuho, Homma, Keiichi, Kasprzyk, Arek, Nishikawa, Tetsuo, Hirakawa, Mika, Thierry-Mieg, Jean, Thierry-Mieg, Danielle, Ashurst, Jennifer, Jia, Libin, Nakao, Mitsuteru, Thomas, Michael A, Mulder, Nicola, Karavidopoulou, Youla, Jin, Lihua, Kim, Sangsoo, Yasuda, Tomohiro, Lenhard, Boris, Eveno, Eric, Suzuki, Yoshiyuki, Yamasaki, Chisato, Takeda, Jun-ichi, Gough, Craig, Hilton, Phillip, Fujii, Yasuyuki, Sakai, Hiroaki, Tanaka, Susumu, Amid, Clara, Bellgard, Matthew, Bonaldo, Maria de Fatima, Bono, Hidemasa, Bromberg, Susan K, Brookes, Anthony J, Bruford, Elspeth, Carninci, Piero, Chelala, Claude, Couillault, Christine, de Souza, Sandro J., Debily, Marie-Anne, Devignes, Marie-Dominique, Dubchak, Inna, Endo, Toshinori, Estreicher, Anne, Eyras, Eduardo, Fukami-Kobayashi, Kaoru, R. Gopinath, Gopal, Graudens, Esther, Hahn, Yoonsoo, Han, Michael, Han, Ze-Guang, Hanada, Kousuke, Hanaoka, Hideki, Harada, Erimi, Hashimoto, Katsuyuki, Hinz, Ursula, Hirai, Momoki, Hishiki, Teruyoshi, Hopkinson, Ian, Imbeaud, Sandrine, Inoko, Hidetoshi, Kanapin, Alexander, Kaneko, Yayoi, Kasukawa, Takeya, Kelso, Janet, Kersey, Paul, Kikuno, Reiko, Kimura, Kouichi, Korn, Bernhard, Kuryshev, Vladimir, Makalowska, Izabela, Makino, Takashi, Mano, Shuhei, Mariage-Samson, Regine, Mashima, Jun, Matsuda, Hideo, Mewes, Hans-Werner, Minoshima, Shinsei, Nagai, Keiichi, Nagasaki, Hideki, Nagata, Naoki, Nigam, Rajni, Ogasawara, Osamu, Ohara, Osamu, Ohtsubo, Masafumi, Okada, Norihiro, Okido, Toshihisa, Oota, Satoshi, Ota, Motonori, Ota, Toshio, Otsuki, Tetsuji, Piatier-Tonneau, Dominique, Poustka, Annemarie, Ren, Shuang-Xi, Saitou, Naruya, Sakai, Katsunaga, Sakamoto, Shigetaka, Sakate, Ryuichi, Schupp, Ingo, Servant, Florence, Sherry, Stephen, Shiba, Rie, Shimizu, Nobuyoshi, Shimoyama, Mary, Simpson, Andrew J, Soares, Bento, Steward, Charles, Suwa, Makiko, Suzuki, Mami, Takahashi, Aiko, Tamiya, Gen, Tanaka, Hiroshi, Taylor, Todd, Terwilliger, Joseph D, Unneberg, Per, Veeramachaneni, Vamsi, Watanabe, Shinya, Wilming, Laurens, Yasuda, Norikazu, Yoo, Hyang-Sook, Stodolsky, Marvin, Makalowski, Wojciech, Go, Mitiko, Nakai, Kenta, Takagi, Toshihisa, Kanehisa, Minoru, Sakaki, Yoshiyuki, Quackenbush, John, Okazaki, Yasushi, Hayashizaki, Yoshihide, Hide, Winston, Chakraborty, Ranajit, Nishikawa, Ken, Sugawara, Hideaki, Tateno, Yoshio, Chen, Zhu, Oishi, Michio, Tonellato, Peter, Apweiler, Rolf, Okubo, Kousaku, Wagner, Lukas, Wiemann, Stefan, Strausberg, Robert L, Isogai, Takao, Auffray, Charles, Nomura, Nobuo, Gojobori, Takashi, Sugano, Sumio
    Publicado 2004
    “…We thus performed an exhaustive integrative characterization of 41,118 full-length cDNAs that capture the gene transcripts as complete functional cassettes, providing an unequivocal report of structural and functional diversity at the gene level. …”
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    Integrative Annotation of 21,037 Human Genes Validated by Full-Length cDNA Clones
  10. 7790
    “…In Nigeria and Burkina Faso, RDT cassettes and blood smears were re-read by an experienced investigator at study end. …”
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    Malaria Rapid Diagnostic Tests and Malaria Microscopy for Guiding Malaria Treatment of Uncomplicated Fevers in Nigeria and Prereferral Cases in 3 African Countries
  11. 7791
    “…The soybean Williams 82 was transformed with the binary CRISPR/Cas9 plasmid, assembled with four sgRNA expression cassettes driven by the Arabidopsis thaliana U3 or U6 promoter, targeting different sites of these four SPL9 genes via Agrobacterium tumefaciens-mediated transformation. …”
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    CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean
  12. 7792
    por Kukil, Kateryna, Lindberg, Pia
    Publicado 2022
    “…To further improve the titer of target compounds, we evaluated the use of stronger expression cassettes for increasing PAL protein levels, as well as knock-out of the laccase gene slr1573, as this was previously reported to prevent degradation of the target compounds in the cell. …”
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    Expression of phenylalanine ammonia lyases in Synechocystis sp. PCC 6803 and subsequent improvements of sustainable production of phenylpropanoids
  13. 7793
    por Wenz, Jan, Arndt, Felix, Samnick, Samuel
    Publicado 2022
    “…BACKGROUND: The GMP-compliant production of radiopharmaceuticals has been performed using disposable units (cassettes) with a dedicated synthesis module. To expand this “plug ‘n’ synthesize” principle to a broader scope of modules we developed a pressure controlled setup that offers an alternative to the usual stepper motor controlled rotary valves. …”
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    A new concept for the production of (11)C-labelled radiotracers
  14. 7794
    “…Subsequently, six recombinant strains based on four constitutive promoters were constructed in variable expression cassettes for improving the expression level of protein. …”
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    Efficient D-allulose synthesis under acidic conditions by auto-inducing expression of the tandem D-allulose 3-epimerase genes in Bacillus subtilis
  15. 7795
    “…RESULTS: By changing the design of the expression cassettes for the rTCA pathway, overexpressing PYC2, and adding CaCO(3) to the batch fermentations, an SA yield on glycerol of 0.63 Cmol Cmol(−1) was achieved (i.e. 47.1% of the theoretical maximum). …”
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    Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae
  16. 7796
    “…We described a clonal dissemination of fosA3-positive IncR-IncF33:A-:B- multireplicon plasmid carrying the gene cassettes IS26-ΔTn3-ISKpn27-bla(KPC-2)-ΔISKpn6-korC-klcA-ΔrepB-Tn1721 in all ST11 isolates. …”
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    Tandem Repeat of bla(NDM-1) and Clonal Dissemination of a fosA3 and bla(KPC-2) Co-Carrying IncR-F33: A–: B– Plasmid in Klebsiella pneumoniae Isolates Collected in a Southwest Hospital in China, 2010–2013
  17. 7797
    “…Certain autophagy gene “cassettes” also participate in non-canonical processes to mediate important biological activities. …”
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    Myeloid autophagy genes protect mice against fatal TNF- and LPS-induced cytokine storm syndromes
  18. 7798
    “…The stably transfected cells, using either constitutive mesenchymal stem cell (MSC) or inducible MSC cassettes, were assayed for their RS1 secretion profile. …”
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    An ex vivo gene therapy approach in X-linked retinoschisis
  19. 7799
    “…The BD-2 lineage contained significantly more SNPs and indels, and showed richness in gene abundance, including antimicrobial resistance genes, gene cassettes, and PLE to fight against bacteriophage infection, acquired over time. …”
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    Genomic Characteristics of Recently Recognized Vibrio cholerae El Tor Lineages Associated with Cholera in Bangladesh, 1991 to 2017
  20. 7800
    “…CONCLUSIONS: Unlike in T cells, the robust activation-driven inducible expression of genetic cassettes in NK cells requires unbiased genome-wide identification of promoter sequences. …”
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    Design and analysis of stably integrated reporters for inducible transgene expression in human T cells and CAR NK-cell lines
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