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122“…A panel of LoxP parent 293 cell lines was generated, each containing a GFP expression cassette under the control of a tetracycline-regulated promoter inserted at a random genome location; the cassette also contained a LoxP site between the promoter and GFP sequence. …”
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123por Tsafnat, Guy, Coiera, Enrico, Partridge, Sally R, Schaeffer, Jaron, Iredell, Jon R“…The algorithm was applied to the discovery of gene cassettes associated with integrons. The discovery and annotation of antibiotic resistance genes in such cassettes is essential for effective monitoring of antibiotic resistance patterns and formulation of public health antibiotic prescription policies. …”
Publicado 2009
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125por Liu, Shumin, Zhou, Shun, Tian, Ling, Guo, Enen, Luan, Yunxia, Zhang, Jianzhen, Li, Sheng“…BACKGROUND: The ATP-binding cassette (ABC) transporter superfamily is the largest transporter gene family responsible for transporting specific molecules across lipid membranes in all living organisms. …”
Publicado 2011
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126“…Here we describe balancer chromosomes that can be directly targeted with transgenes of interest via recombinase-mediated cassette exchange (RMCE).…”
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127por Badri, Dayakar V., Chaparro, Jacqueline M., Manter, Daniel K., Martinoia, Enrico, Vivanco, Jorge M.“…Here, we report the involvement of seven root-expressed ATP-binding cassette (ABC) transporters corresponding to both full and half-size molecules (Atabcg36, Atabcg37, Atabcc5, Atabcf1, Atabcf3, Atnap5, and Atath10) in root exudation processes using Arabidopsis thaliana. …”
Publicado 2012
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128por Domingues, Sara, Harms, Klaus, Fricke, W. Florian, Johnsen, Pål J., da Silva, Gabriela J., Nielsen, Kaare Magne“…Moreover, natural transformation of integron harboring populations of competent bacteria revealed that interspecies exchange of gene cassettes can be highly efficient, and independent on genetic relatedness between donor and recipient. …”
Publicado 2012
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129por Sureshan, Visaahini, Deshpande, Chandrika N., Boucher, Yan, Koenig, Jeremy E., Stokes, H. W., Harrop, Stephen J., Curmi, Paul M. G., Mabbutt, Bridget C.“…In most cases, functional annotation of gene cassettes directly recovered by cassette-PCR is obscured by their characteristically high sequence novelty. …”
Publicado 2013
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130Functional analysis of the ATP-binding cassette (ABC) transporter gene family of Tribolium castaneum“…BACKGROUND: The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. …”
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131por Zhao, Hongtao, Liu, Lei, Mo, Huixian, Qian, Litao, Cao, Ying, Cui, Sujuan, Li, Xia, Ma, Ligeng“…In these processes, the concentration gradient of auxin, which is established by polar auxin transport mediated by PIN-FORMED (PIN) proteins and several ATP-binding cassette/multi-drug resistance/P-glycoprotein (ABCB/MDR/PGP) transporters, is a crucial signal. …”
Publicado 2013
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133“…They contain two yeast expression cassettes driven by either (1) the constitutive TEF1 and PGK1 promoters, or (2) the constitutive TEF1 promoter and the inducible GAL10 or HXT7 promoters. …”
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134“…The most common cause of MDR is considered to be the increased expression of adenosine triphosphate binding cassette (ABC) transporters. Numerous studies have identified that the upregulation of ABC transporters is often observed following treatment with HDAC inhibitors, particularly the increased expression of P-glycoprotein, which leads to drug efflux, reduces intracellular drug concentration and induces MDR. …”
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135“…To expand the toolbox for genomic modifications, we designed a strategy based on the Cre/loxP-based Recombinase-Mediated Cassette Exchange (RMCE) system for functional genomics analyses. …”
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136por Haghighat-Khah, Roya Elaine, Scaife, Sarah, Martins, Sara, St John, Oliver, Matzen, Kelly Jean, Morrison, Neil, Alphey, Luke“…Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. …”
Publicado 2015
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137por Yan, Ming, Wen, Jing, Liang, Min, Lu, Yunfeng, Kamata, Masakazu, Chen, Irvin S. Y.“…For proof of concept, we transduced DNA expression cassettes for three small RNAs. In one application, the DNA cassette encodes an shRNA transcriptional unit that downregulates CCR5 and protects from HIV-1 infection. …”
Publicado 2015
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138por Sugiyama, Akifumi, Fukuda, Shoju, Takanashi, Kojiro, Yoshioka, Miki, Yoshioka, Hirofumi, Narusaka, Yoshihiro, Narusaka, Mari, Kojima, Mikiko, Sakakibara, Hitoshi, Shitan, Nobukazu, Sato, Shusei, Tabata, Satoshi, Kawaguchi, Masayoshi, Yazaki, Kazufumi“…LjABCG1, a full-size ABCG subfamily of ATP-binding cassette proteins of a model legume, Lotus japonicus, was reported as a gene highly expressed during the early stages of nodulation, but have not been characterized in detail. …”
Publicado 2015
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139Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes“…We have created a toolkit of template-mediated repair cassettes that contain an antibiotic resistance gene to select for worms carrying the repair template and a fluorescent visual marker that facilitates identification of bona fide recombinant animals. …”
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140por Bartosik, Aneta A., Glabski, Krzysztof, Kulinska, Anna, Lewicka, Ewa, Godziszewska, Jolanta, Markowska, Aleksandra, Jagura-Burdzy, Grazyna“…CONCLUSION: The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.…”
Publicado 2016
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