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  1. 2061
    por Traore, Sy Mamadou, Zhao, Bingyu
    Publicado 2011
    “…BACKGROUND: Cloning genes into plasmid vectors is one of the key steps for studying gene function. …”
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    A novel Gateway(®)-compatible binary vector allows direct selection of recombinant clones in Agrobacterium tumefaciens
  2. 2062
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    Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone
  3. 2063
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    Cloning and Expression Analysis of a PISTILLATA Homologous Gene from Pineapple (Ananas comosus L. Merr)
  4. 2064
    “…The maximum size and age that clonal organisms can reach remains poorly known, although we do know that the largest natural clones can extend over hundreds or thousands of metres and potentially live for centuries. …”
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    Implications of Extreme Life Span in Clonal Organisms: Millenary Clones in Meadows of the Threatened Seagrass Posidonia oceanica
  5. 2065
    “…Xylanases are digestive enzymes responsible for the degradation of xylan based polymers, hydrolyzing them into smaller molecules that are easier to assimilate by insects. We report the cloning, expression and enzymatic characterization of a xylanase gene that was identified in the digestive tract of the coffee berry borer. …”
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    Cloning and expression of an endo-1,4-β-xylanase from the coffee berry borer, Hypothenemus hampei
  6. 2066
    “…Consequently, our results show that SNP-typing may be useful to identify and track MRSA clones that are specific to individual healthcare institutions. …”
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    Single-Nucleotide Polymorphism Genotyping Identifies a Locally Endemic Clone of Methicillin-Resistant Staphylococcus aureus
  7. 2067
    “…Construction of rescue clones using gap repair cloning is highly efficient and provides a novel use of the homologous recombination-based method in E. coli for molecular cloning, known colloquially as recombineering, when rescuing large BAC deletions. …”
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    Use of Recombination-Mediated Genetic Engineering for Construction of Rescue Human Cytomegalovirus Bacterial Artificial Chromosome Clones
  8. 2068
    “…Amino acid sequence analysis revealed that the cloned HC29 cDNA contained the conserved catalytic triad and dimer interface of selenium-independent glutathione peroxidase (GPX). …”
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    Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus
  9. 2069
    “…Thus, our newly established infectious clone of CSFV, Flc-LOM, could serve as a vaccine candidate.…”
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    Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain
  10. 2070
    por Han, Dandan, Wen, Luan, Chen, Yonglong
    Publicado 2012
    “…Here we report the molecular cloning of phd1 and systematic analysis of phd1, phd2, and phd3 expression in embryos as well as adult tissues of Xenopus laevis. …”
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    Molecular Cloning of phd1 and Comparative Analysis of phd1, 2, and 3 Expression in Xenopus laevis
  11. 2071
    “…The present study describes the molecular cloning, characterization and function of atRA-induced expression of a spliced variant of the CYP26B1 gene. …”
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    Cloning and Functional Studies of a Splice Variant of CYP26B1 Expressed in Vascular Cells
  12. 2072
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    Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
  13. 2073
    “…Recently, we reported the three wolves cloning with normal karyotype from somatic cells of endangered male gray wolves (Canis lupus), but one wolf had female external genitalia. …”
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    SRY-positive 78, XY ovotesticular disorder of sex development in a wolf cloned by nuclear transfer
  14. 2074
    “…Therefore, for the first time we have cloned and characterized two neuropeptide genes (flp-12 and flp-16) from the cDNA library of feeding female of H. avenae. …”
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    Cloning and characterization of two neuropeptide genes from cereal cyst nematode, Heterodera avanae from India
  15. 2075
    “…The accumulation of mutations in asexual clones leads to the establishment of a network of clone mates. …”
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    The Pattern of Genetic Variability in Apomictic Clones of Taraxacum officinale Indicates the Alternation of Asexual and Sexual Histories of Apomicts
  16. 2076
    “…The Gaza strain was closely related genetically to a local MSSA spa t223 strain and less so to EMRSA15, one of the pandemic hospital-acquired-MRSA clones, scarcely reported in the community. The rapid spread in the community may be due to population determinants or due to yet unknown advantageous features of this particular strain.…”
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    A Typical Hospital-Acquired Methicillin-Resistant Staphylococcus aureus Clone Is Widespread in the Community in the Gaza Strip
  17. 2077
    “…These suggest that few but transmissible clones can transmit after acquiring multidrug resistance and colonize even in a country with a developed, well-organized healthcare system.…”
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    Dominant Incidence of Multidrug and Extensively Drug-Resistant Specific Mycobacterium tuberculosis Clones in Osaka Prefecture, Japan
  18. 2078
    “…The atpS gene (1674 bp) encoding the ATPS from Acidithiobacillus ferrooxidans ATCC 23270 was amplified using PCR, cloned in the pET101-TOPO plasmid, sequenced and expressed in Escherichia coli obtaining a 63.5 kDa ATPS recombinant protein according to SDS-PAGE analysis. …”
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    Cloning, expression and bioinformatics analysis of ATP sulfurylase from Acidithiobacillus ferrooxidans ATCC 23270 in Escherichia coli
  19. 2079
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    Molecular Cloning and Functional Analysis of Three FLOWERING LOCUS T (FT) Homologous Genes from Chinese Cymbidium
  20. 2080
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    Cloning of aquaporin-1 of the blue crab, Callinectes sapidus: its expression during the larval development in hyposalinity
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