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  1. 2081
    “…We show that this method can be applied to genotype a collection of clones from natural infections. Furthermore, we show how to cryo-preserve C. bombi cells to be revived to become infective clones after at least 4 years of storage. …”
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    Probing Mixed-Genotype Infections I: Extraction and Cloning of Infections from Hosts of the Trypanosomatid Crithidia bombi
  2. 2082
    “…Over 60% of these single-species mounds had a single aphid clone, and clones tended to persist across subsequent years. …”
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    Ants farm subterranean aphids mostly in single clone groups - an example of prudent husbandry for carbohydrates and proteins?
  3. 2083
    “…However, 22 cultivars were found to consist of multiple clones, which probably originate from the chance seedlings, suggesting that their unique characteristics have not been maintained through propagation by grafting alone. …”
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    Clone identification in Japanese flowering cherry (Prunus subgenus Cerasus) cultivars using nuclear SSR markers
  4. 2084
    “…Using CODEHOP PCR we identified a single P2X receptor (LymP2X) in Lymnaea CNS which was subsequently cloned by RT-PCR. When heterologously expressed in Xenopus oocytes, LymP2X exhibited ATP evoked inward currents (EC(50) 6.2 µM) which decayed during the continued presence of agonist. …”
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    Cloning and Characterization of a P2X Receptor Expressed in the Central Nervous System of Lymnaea stagnalis
  5. 2085
    “…CONCLUSIONS/SIGNIFICANCE: In the present study, a fungal nitrilase was cloned from the cDNA sequence of G. intermedia and successfully expressed in E. coli Rosetta-gami (DE3). …”
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    Fungal His-Tagged Nitrilase from Gibberella intermedia: Gene Cloning, Heterologous Expression and Biochemical Properties
  6. 2086
    “…The transcript levels of BEX1 and XIST were upregulated and PGK1 was downregulated in both IVF and cloned blastocysts compared with in vivo counterparts. …”
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    X-Linked Gene Transcription Patterns in Female and Male In Vivo, In Vitro and Cloned Porcine Individual Blastocysts
  7. 2087
    por Wilde, Susanne, Schendel, Dolores J.
    Publicado 2012
    “…Recently, we compared 12 HLA-A2-restricted, tyrosinase peptide-specific CD8(+) cytotoxic T-lymphocyte (CTL) clones and demonstrated that polyfunctional type 1 helper (Th1)-cytokine secretion serves to rapidly select high-quality, high-avidity CTLs.…”
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    High-quality and high-avidity T cell clones specific for tumor-associated antigens and how to find them
  8. 2088
    “…RESULTS: In order to investigate OtrC’s function, otrC is cloned and expressed in E. coli The exporter function of OtrC was identified by ATPase activity determination and ethidium bromide efflux assays. …”
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    Molecular cloning and functional characterization of an ATP-binding cassette transporter OtrC from Streptomyces rimosus
  9. 2089
    “…We established a new field clone of Plasmodium falciparum for use in controlled human malaria infections and vaccine studies to complement the current small portfolio of P. falciparum strains, primarily based on NF54. …”
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    NF135.C10: A New Plasmodium falciparum Clone for Controlled Human Malaria Infections
  10. 2090
    “…In order to study the biochemical properties of the β-galactosidase enzyme, the bga gene was PCR amplified, cloned, and expressed in the genetically tractable haloarchaeon Halobacterium sp. …”
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    Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
  11. 2091
    “…A highly efficient cloning vector was constructed for cloning PCR products by inserting an 80 bp DNA fragment into pGEM-5zf (+) vector. …”
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    Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)
  12. 2092
    “…To test the acute effects of both glyphosate and a commercial formulation of Roundup (hereafter Roundup), we conducted a series of exposure experiments with different clones and age-classes of D. magna. The results demonstrated EC(50) (48) values in the low ppm-range for Roundup as well as for the active ingredient (a.i.) isopropylamine salt of glyphosate (glyphosate IPA) alone. …”
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    Clone- and age-dependent toxicity of a glyphosate commercial formulation and its active ingredient in Daphnia magna
  13. 2093
    “…When comparing damaged plants, the S. dasyclados clone was more attractive to A. nemorum than the S. viminalis clone—a somewhat surprising result since this Salix clone is considered relatively resistant to P. vulgatissima, and hence offers a limited amount of prey. …”
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    Constitutive and herbivore-induced systemic volatiles differentially attract an omnivorous biocontrol agent to contrasting Salix clones
  14. 2094
    “…The fibroblasts, designated DS1 cells, are a cloned population of immortalized cells isolated from a primary culture of marrow stromal cells. …”
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    Late Infusion of Cloned Marrow Fibroblasts Stimulates Endogenous Recovery from Radiation-Induced Lung Injury
  15. 2095
    “…There is no size restriction for gene insertion, clone efficiency is > 95% with clone numbers up to 200. …”
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    A new method to customize protein expression vectors for fast, efficient and background free parallel cloning
  16. 2096
    “…By using this novel OLTA (OverLap extension PCR and TA-cloning) method, arbitrary ZFN vectors were synthesized within 3 days, from the designing to the sequencing of the vector. …”
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    Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning
  17. 2097
    “…Laccases (EC 1.10.3.2) are copper-containing polyphenol oxidases found in white-rot fungi. Here, we report the cloning and analysis of the nucleotide sequence of a new laccase gene, fvlac7, based on the genomic sequence of Flammulina velutipes. …”
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    Cloning and Characterization of a Novel Laccase Gene, fvlac7, Based on the Genomic Sequence of Flammulina velutipes
  18. 2098
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    Genomic Estimated Breeding Values Using Genomic Relationship Matrices in a Cloned Population of Loblolly Pine
  19. 2099
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    Skin Stem Cell Hypotheses and Long Term Clone Survival – Explored Using Agent-based Modelling
  20. 2100
    “…Bacterial nucleic acids were subjected to terminal restriction fragment (TRF) length polymorphism and clone library analysis. Overall, we identified 326 T-RFLP band, 159 in patients and 167 in healthy controls. …”
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    Analysis of the Airway Microbiota of Healthy Individuals and Patients with Chronic Obstructive Pulmonary Disease by T-RFLP and Clone Sequencing
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