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  1. 2121
    “…Here, we address recently developed restriction-free methods in cloning, and present a more cost-efficient protocol that has been optimized to improve both cloning and clone screening. …”
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    A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement
  2. 2122
    “…RESULTS: A Pel gene (pelN) was cloned using degenerate PCR and inverse PCR from the chromosomal DNA of Paenibacillus sp. 0602. …”
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    Cloning, expression and characterization of a pectate lyase from Paenibacillus sp. 0602 in recombinant Escherichia coli
  3. 2123
    “…Two new indole alkaloids, metagenetriindole A (1) and metagenebiindole A (2), were identified from deep-sea sediment metagenomic clone derived Escherichia coli fermentation broth. …”
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    Two New Cytotoxic Indole Alkaloids from a Deep-Sea Sediment Derived Metagenomic Clone
  4. 2124
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    Massive depletion of BLV proviral clones located in genomic transcriptionally active sites during primary infection
  5. 2125
    “…The gene was PCR amplified, cloned and successfully expressed in E. coli. The expressed recombinant enzyme was purified using metal affinity chromatography. …”
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    Cloning, expression and characterization of a versatile Baeyer-Villiger monooxygenase from Dietzia sp. D5
  6. 2126
    “…To improve this efficiency in the production of cloned pigs, it is critical to understand the interactions between uterine function and cloned embryos during implantation. …”
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    Analysis of ENPP2 in the Uterine Endometrium of Pigs Carrying Somatic Cell Nuclear Transfer Cloned Embryos
  7. 2127
    “…Although expression rates of hla were in general strain-specific, we observed CA clones showed significantly higher hla expression (p = 0.003) when compared with HA clones. …”
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    Insights into Alpha-Hemolysin (Hla) Evolution and Expression among Staphylococcus aureus Clones with Hospital and Community Origin
  8. 2128
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    Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178
  9. 2129
    “…The mannanase gene of B. circulans NT 6.7 was cloned and expressed in an Escherichia coli expression system. …”
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    Cloning, secretory expression and characterization of recombinant β-mannanase from Bacillus circulans NT 6.7
  10. 2130
    “…The hybrid progeny appeared to be triploid based on DNA content analysis. The hybrid clone analyzed was stable throughout the complete parasite life cycle. …”
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    First Evidence of Intraclonal Genetic Exchange in Trypanosomatids Using Two Leishmania infantum Fluorescent Transgenic Clones
  11. 2131
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    SubcloneSeeker: a computational framework for reconstructing tumor clone structure for cancer variant interpretation and prioritization
  12. 2132
    “…In the present study, we cloned and characterized the tesk1 gene of tongue sole, Cynoglossus semilaevis. …”
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    Cloning and Characterization of tesk1, a Novel Spermatogenesis-Related Gene, in the Tongue Sole (Cynoglossus semilaevis)
  13. 2133
    “…RESULTS: In this study, wingless gene (Wg) was cloned from three species of rice planthopper, Sogatella furcifera, Laodelphgax striatellus and Nilaparvata lugens, and its characteristics and role in determining the wing dimorphism of S. furcifera were explored. …”
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    Wingless gene cloning and its role in manipulating the wing dimorphism in the white-backed planthopper, Sogatella furcifera
  14. 2134
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    Cloning of a synthetic chimeric gene containing recombinant Mycoplasma hyopneumoniae antigens for expression in Pichia pastoris
  15. 2135
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    Cloning andexpression of cDNA encoding growth hormone tambaqui (Colossoma macropomum) in the yeast Pichia pastoris
  16. 2136
    “…The SlRBR cDNAs were cloned into the pBS plasmid and sequenced. Both 5′- and 3′-RACE were generated and sequenced. …”
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    Cloning and Expression Profiling of the Polycomb Gene, Retinoblastoma-related Protein from Tomato Solanum lycopersicum L.
  17. 2137
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    Cloning of Insertion Site Flanking Sequence and Construction of Transfer DNA Insert Mutant Library in Stylosanthes Colletotrichum
  18. 2138
    “…However, current mutagenesis cloning protocols require Sanger sequencing, and thus are prohibitively costly and labor-intensive. …”
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    A Massively Parallel Pipeline to Clone DNA Variants and Examine Molecular Phenotypes of Human Disease Mutations
  19. 2139
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    Cloning of the Cryptochrome-Encoding PeCRY1 Gene from Populus euphratica and Functional Analysis in Arabidopsis
  20. 2140
    “…Molecular cloning is utilized in nearly every facet of biological and medical research. …”
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    Hot Fusion: An Efficient Method to Clone Multiple DNA Fragments as Well as Inverted Repeats without Ligase
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