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  1. 2221
    “…RESULTS: From cold-treated H. compressa plants, a putative DREB2 gene (HcDREB2) was cloned using the RACE-PCR method. HcDREB2 was 1296 bp in length and encoded a putative protein 264 amino acid residues long. …”
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    Cloning and characterization of an ABA-independent DREB transcription factor gene, HcDREB2, in Hemarthria compressa
  2. 2222
    “…Two students carried different MRSA clones at various times and persistent MRSA carriage was noted in one student. …”
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    Hospital clones of methicillin-resistant Staphylococcus aureus are carried by medical students even before healthcare exposure
  3. 2223
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    Dynamic variation of CD5 surface expression levels within individual chronic lymphocytic leukemia clones
  4. 2224
    “…In conclusion, compared to the traditional RIA method, the ECLI method is a safe and reliable method for canine cloning.…”
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    Dog cloning with in vivo matured oocytes obtained using electric chemiluminescence immunoassay-predicted ovulation method
  5. 2225
    “…Heterologous expression of BGCs has become a useful strategy to produce, reactivate, improve, and modify the pathways of NPs present at minute quantities in the original actinomycetes isolates. However, cloning and efficient overexpression of an entire NP BGC, often as large as over 100 kb, remain challenging due to the ineffectiveness of current genetic systems in manipulating large NP BGCs. …”
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    Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster in Streptomyces Species
  6. 2226
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    Persistence of a t(11;14)‐positive clone in a patient with mantle cell lymphoma for 20 years
  7. 2227
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    Genomics Reveals a Unique Clone of Burkholderia cenocepacia Harboring an Actively Excising Novel Genomic Island
  8. 2228
    “…To enhance genetic manipulation of Streptococcus sanguinis, we have developed a cloning-independent methodology, which uses a counterselectable marker and allows sophisticated markerless gene editing in situ. …”
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    Cloning-independent markerless gene editing in Streptococcus sanguinis: novel insights in type IV pilus biology
  9. 2229
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    QuickLib, a method for building fully synthetic plasmid libraries by seamless cloning of degenerate oligonucleotides
  10. 2230
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    Cloning retinoid and peroxisome proliferator-activated nuclear receptors of the Pacific oyster and in silico binding to environmental chemicals
  11. 2231
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    Cloning and Functional Characterization of a Lycopene β-Cyclase from Macrophytic Red Alga Bangia fuscopurpurea
  12. 2232
    “…Thus, purified recombinant human BSSL (rhBSSL) can be used for treatment of patients with fat malabsorption and expressing rhBSSL in the milk of transgenic cloned cows would therefore be a mean to meet a medical need. …”
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    Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows
  13. 2233
    “…As part of our own efforts to further improve upon the S. mutans genetic toolbox, we previously reported the development of the first cloning-independent markerless mutagenesis (CIMM) system for S. mutans and illustrated how this approach could be adapted for use in many other organisms. …”
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    Recombineering in Streptococcus mutans Using Direct Repeat-Mediated Cloning-Independent Markerless Mutagenesis (DR-CIMM)
  14. 2234
    “…RESULTS: To reveal their susceptibility differences, we firstly performed herpesvirus challenge experiments in three gynogenetic clones of gibel carp, including the leading variety clone A(+), candidate variety clone F and wild clone H. …”
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    Distinct herpesvirus resistances and immune responses of three gynogenetic clones of gibel carp revealed by comprehensive transcriptomes
  15. 2235
    “…This single patient experience highlights the risk of T315I clone selection in Ph+ ALL treated with reduced dose ponatinib.…”
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    T315I clone selection in a Ph+ all patient under low‐dose ponatinib maintenance
  16. 2236
    “…By this approach, in a real experimental setting, we disrupted the Aicda gene in ~70% of clones from CH12F3 lymphoma cells (>40% biallelically). …”
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    An efficient method to enrich for knock-out and knock-in cellular clones using the CRISPR/Cas9 system
  17. 2237
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    Molecular cloning and characterization of protein disulfide isomerase of Brugia malayi, a human lymphatic filarial parasite
  18. 2238
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    A novel inducible mutagenesis screen enables to isolate and clone both embryonic and adult zebrafish mutants
  19. 2239
    “…To overcome these limitations, we adapted synthetic biology tools to clone CMV genomes in Saccharomyces cerevisiae. Using an early passage of the human CMV isolate Toledo, we first applied transformation-associated recombination (TAR) to clone 16 overlapping fragments covering the entire Toledo genome in Saccharomyces cerevisiae. …”
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    Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
  20. 2240
    “…Recombinant protein (18 kDa) expressed by the cloned Vg gene was characterized, and the effects of the expression of this protein on the physiology of H. axyridis were investigated. …”
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    Molecular Cloning of the Vitellogenin Gene and the Effects of Vitellogenin Protein Expression on the Physiology of Harmonia axyridis (Coleoptera: Coccinellidae)
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