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Biología molecular
5
Clonación molecular
3
Ingeniería genética
3
Biología
2
Bioquímica
2
Biotecnología
2
Clonamiento molecular
2
Fisiología
2
Genética humana
2
ADN
1
Biotecnología agrícola
1
Carbohidratos
1
Cerveza
1
Ciencia ficción
1
Clasificación
1
Clonación
1
Clone had no significant effect on specify gravity or moisture content
1
Creación literaria, artística, etc
1
Cromosomas
1
Cromosomas sexuales
1
Cuentos argentinos
1
Cultivo de células
1
Células madre
1
Determinación del sexo
1
El análisis de ranking evidenció cuatro clones superiores con potencial de mejoramiento
1
El clon no tuvo efecto significativo sobre el peso específico ni el contenido de humedad
1
Epigénesis
1
Estética
1
Evolución (Biología)
1
Genes
1
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2322por Wang, Xiaona, Li, Fengsai, Han, Meijing, Jia, Shuo, Wang, Li, Qiao, Xinyuan, Jiang, Yanping, Cui, Wen, Tang, Lijie, Li, Yijing, Xu, Yi-Gang“…Nevertheless, there is limited knowledge regarding feline IFN-ω (feIFN-ω), compared to IFN-α and IFN-β. In this study, we cloned the genes encoding feIFN-ωa and feIFN-ωb from cat spleen lymphocytes. …”
Publicado 2020
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2323por Shola, Dorjee T.N., Yang, Chingwen, Kewaldar, Vhy-Shelta, Kar, Pradip, Bustos, Victor“…Here, we demonstrate two additions to the repertoire of CRISPR's application for constructing donor DNA templates: CRISPR-CLONInG and CRISPR-CLIP. CRISPR-CLONInG (CRISPR-Cutting and Ligation Of Nucleic acid In vitro via Gibson) was devised to enable efficient cut-and-paste of multiple complex DNA fragments by using CRISPR-Cas9 as a digestion alternative with precision and exclusivity features, followed by joining the digested products via Gibson Assembly, to construct double-stranded DNA and adeno-associated virus (AAV) donor vectors rapidly without cloning scars. …”
Publicado 2020
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2324por Psaila, Bethan, Wang, Guanlin, Rodriguez-Meira, Alba, Li, Rong, Heuston, Elisabeth F., Murphy, Lauren, Yee, Daniel, Hitchcock, Ian S., Sousos, Nikolaos, O’Sullivan, Jennifer, Anderson, Stacie, Senis, Yotis A., Weinberg, Olga K., Calicchio, Monica L., Iskander, Deena, Royston, Daniel, Milojkovic, Dragana, Roberts, Irene, Bodine, David M., Thongjuea, Supat, Mead, Adam J.“…Our study paves the way for selective targeting of the myelofibrosis clone and illustrates the power of single-cell multi-omics to discover tumor-specific therapeutic targets and mediators of tissue fibrosis.…”
Publicado 2020
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2325por Carrami, Eli M, Sharifzadeh, Sahand, Wietek, Nina C, Artibani, Mara, El-Sahhar, Salma, Sauka-Spengler, Tatjana, Yau, Christopher, Tresp, Volker, Ahmed, Ahmed A“…Overall, we propose DigiPico/MutLX method as a powerful framework for the identification of clone-specific variants at an unprecedented accuracy.…”
Publicado 2020
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2326por Wang, Zhi-Peng, Cao, Min, Li, Bing, Ji, Xiao-Feng, Zhang, Xin-Yue, Zhang, Yue-Qi, Wang, Hai-Ying“…Then, the gene ALYW201 was cloned from Vibrio sp. W2 and expressed in a food-grade host, Yarrowia lipolytica. …”
Publicado 2020
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2327por Gorlenko, Cyrill L., Kiselev, Herman Yu., Budanova, Elena V., Zamyatnin, Andrey A., Ikryannikova, Larisa N.Enlace del recurso
Publicado 2020
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2328por Kamada, Toshiki, Une, Yumi, Matsui, Kumi, Fuma, Shoichi, Ikeda, Teruo, Okamoto, Mariko“…We confirmed that the cloned cDNAs were able to synthesize salamander proteins by western blotting after transfection into cultured HEK293 cells. …”
Publicado 2020
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2329“…These markers are removable if desired as part of the cloning procedure (with no extra steps needed). We demonstrated utility via cloning of 38 and 22 kb genomic segments from Salmonella enterica serovar Typhimurium and Escherichia coli, respectively. …”
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2330por Yan, Xu, Li, Yingzheng, Wu, Zizhou, Li, Yang, Wen, Xiaodong, Li, Xiaofeng, He, Ruyu, Yang, Chunyan, Zhao, Yanli, Cheng, Mingjun, Zhang, Ping, Sam, Ebenezer Kofi, Rong, Tingzhao, He, Jianmei, Tang, QilinEnlace del recurso
Publicado 2020
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2331“…Finally, the full-length cDNA of mr-magR was successfully cloned and expressed in Escherichia coli BL21 (DE3), and the Mr-MagR protein was purified by a Ni(+)-NTA column and identified by Western blot. …”
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2332por Yang, Lang, Lü, Bing, Wang, Quanyi, Wang, Kaiying, Lin, Yanfeng, Yang, Chaojie, Qiu, Shaofu, Li, Peng, Song, Hongbin“…Interregional spread, rapid local expansion, and acquirement of antimicrobial resistance determinants have cocontributed to the epidemics of this clone. Another ST18 clone was also identified and showed long-term colonization in Beijing. …”
Publicado 2020
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2333por Breidenbach, Natalie, Sharov, Vadim V., Gailing, Oliver, Krutovsky, Konstantin V.“…To study genetic mechanisms of frost resistance in this species and to select the most frost tolerant trees we tested 17 clones in climate control chamber experiments and generated two de novo assemblies of the coast redwood transcriptome from a pooled RNA sample using Trinity and CLC Genomic Workbench software, respectively. …”
Publicado 2020
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2334“…Surface expression of PD-L1 was not observed on neutrophils from healthy volunteers or patients with COPD when clone 29E.2A3 of anti-PD-L1 was not used with AlexaFluor™700-conjugated anti-CD16. …”
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2335por McCracken, Melissa, Mitchell, Robyn, Smith, Stephanie, Hota, Susy, Conly, John, Du, Tim, Embil, John, Johnston, Lynn, Ormiston, Debbie, Parsonage, Jennifer, Simor, Andrew, Wong, Alice, Golding, GeorgeEnlace del recurso
Publicado 2020
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2336“…Producing androgenetic diploid embryos could be an outstanding strategy; androgenic diploid embryos are produced through double sperm cloning (DSC), in which two capacitated sperms (XY or XX, sorted by flow cytometer) are injected into a denucleated oocyte by intracytoplasmic sperm injection (ICSI) to reconstruct embryo and derive DSC-ESCs. …”
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2337por Kruse, Stefan, Kolmogorov, Aleksey I., Pestryakova, Luidmila A., Herzschuh, Ulrike“…Findings reveal that the predominant reproduction type is clonal (58.76%) by short distance spreading of ramets. One outlier of clones 1 km apart could have been dispersed by reindeer. …”
Publicado 2020
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2338“…Alphaviruses (genus Alphavirus; family Togaviridae) are a medically relevant family of viruses that include chikungunya virus and Mayaro virus. Infectious cDNA clones of these viruses are necessary molecular tools to understand viral biology. …”
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2339por Ambroa, Antón, Blasco, Lucia, López-Causapé, Carla, Trastoy, Rocio, Fernandez-García, Laura, Bleriot, Ines, Ponce-Alonso, Manuel, Pacios, Olga, López, Maria, Cantón, Rafael, Kidd, Timothy J., Bou, German, Oliver, Antonio, Tomás, Maria“…In addition, we identified a new filamentous prophage, designated Pf8, in the P. aeruginosa AUS411.500 isolate belonging to the international CF clone. We detected only one prophage, never previously described, from the family Siphoviridiae (with 66 proteins and displaying homology with PHAGE_Pseudo_phi297_NC_016762). …”
Publicado 2020
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2340por Stocker, Franz, Obermeier, Melanie M., Resch, Katharina, Berg, Gabriele, Müller Bogotá, Christina A.“…This led to the identification of five clones that inhibited the growth of mycelium and spores in vitro by up to 8% and 30% and subsequently, to the identification of VOCs that are potentially, and in part responsible for the clones’ antifungal activity. …”
Publicado 2020
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