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  1. 2481
    “…Sequence analysis of random clones from the library identified 14 miRNAs, 4 of which are novel to rainbow trout. …”
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    Cloning and characterization of microRNAs from rainbow trout (Oncorhynchus mykiss): Their expression during early embryonic development
  2. 2482
    “…Here a series of ligation independent cloning based vectors were constructed to address this challenge. …”
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    Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis
  3. 2483
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    Cloning of the cytochrome p450 reductase (crtR) gene and its involvement in the astaxanthin biosynthesis of Xanthophyllomyces dendrorhous
  4. 2484
    “…CONCLUSION: We have isolated the Ehvma2 gene which encodes for the V-ATPase subunit B from the E. histolytica clone A. This gene has a 154 bp intron and encodes for a highly conserved polypeptide. …”
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    The Vacuolar ATPase from Entamoeba histolytica: Molecular cloning of the gene encoding for the B subunit and subcellular localization of the protein
  5. 2485
    “…CONCLUSION: Together, the results suggest that proper organization of constitutive heterochromatin in early embryos is involved in the initial developmental steps and might have long term consequences, especially in cloning procedures.…”
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    Trichostatin A treatment of cloned mouse embryos improves constitutive heterochromatin remodeling as well as developmental potential to term
  6. 2486
    “…Direct cloning and parallel sequencing, an extremely powerful method for microRNA (miRNA) discovery, has not yet been applied to bacterial transcriptomes. …”
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    Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing
  7. 2487
    “…In a family-based study of 76 pedigrees from Dhaka, Bangladesh, we evaluated the association between cholera and five candidate genes -- the cystic fibrosis transmembrane receptor; lactoferrin; long palate, lung and nasal epithelium clone 1 (LPLUNC1); estrogen related receptor alpha; and calcium activated chloride channel 1. …”
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    A Variant in Long Palate, Lung and Nasal Epithelium Clone 1 is Associated with Cholera in a Bangladeshi Population
  8. 2488
    por Nocarova, Eva, Fischer, Lukas
    Publicado 2009
    “…In contrast, the offspring of subsequent cloning of the cloned lines was uniform, showing GFP fluorescence intensity and heterogeneity that corresponded to the original clone. …”
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    Cloning of transgenic tobacco BY-2 cells; an efficient method to analyse and reduce high natural heterogeneity of transgene expression
  9. 2489
    “…Each prevalent emm clone had major PFGE strain(s) and many minor strains. …”
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    Association of the shuffling of Streptococcus pyogenes clones and the fluctuation of scarlet fever cases between 2000 and 2006 in central Taiwan
  10. 2490
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    P20-15. Evaluation of HIV-1 subtype B acute envelope-expressing infectious molecular clones
  11. 2491
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    Wing Defects in Drosophila xenicid Mutant Clones Are Caused by C-Terminal Deletion of Additional Sex Combs (Asx)
  12. 2492
    “…The gene cluster was first reassembled into an integrative plasmid by Red/ET technology combining with classic cloning methods and then the resulting plasmid(pNIK)was introduced into S. ansochromogenes by conjugal transfer. …”
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    Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production
  13. 2493
    “…A 40-kDa protein was purified combining six chromatographic steps and peptides were de novo sequenced. This allowed the cloning of the gene from Arabidopsis thaliana and the expression of the recombinant protein in Escherichia coli for biochemical characterization. …”
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    Cloning of Glucuronokinase from Arabidopsis thaliana, the Last Missing Enzyme of the myo-Inositol Oxygenase Pathway to Nucleotide Sugars
  14. 2494
    “…We investigated the parallel production in a medium throughput strategy of genes coding for proteins from various marine organisms, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. …”
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    MARINE-EXPRESS: taking advantage of high throughput cloning and expression strategies for the post-genomic analysis of marine organisms
  15. 2495
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    Cloning, expression and transmission-blocking activity of anti-PvWARP, malaria vaccine candidate, in Anopheles stephensi mysorensis
  16. 2496
    “…CONCLUSIONS: We have devised a simple, rapid and easy method for generating cDNA clone from genomic sequences. The full length HOXD13 clone (1.1 kb) generated with this technique was confirmed by sequence analysis. …”
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    A simple method for generating full length cDNA from low abundance partial genomic clones
  17. 2497
    “…BACKGROUND: Molecular DNA cloning is crucial to many experiments and with the trend to higher throughput of modern approaches automated techniques are urgently required. …”
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    Automated seamless DNA co-transformation cloning with direct expression vectors applying positive or negative insert selection
  18. 2498
    por Tang, Bin, Wang, Shigui, Zhang, Fan
    Publicado 2010
    “…RESULTS: To gain insight into the function of storage proteins in insects, cDNAs for two storage proteins were cloned from the fat body of Spodoptera exigua. S. exigua hexamerin 1 (SeHex) cDNA contained an open reading frame of 2124 nucleotides encoding a protein of 707 amino acids with a predicted molecular weight of 82.12 kDa. …”
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    Two storage hexamerins from the beet armyworm Spodoptera exigua: Cloning, characterization and the effect of gene silencing on survival
  19. 2499
    “…RESULTS: The genes encoding two new members of family-20 GlcNAcases were isolated from the genome of V. harveyi 650, cloned and expressed at a high level in E. coli. VhNag1 has a molecular mass of 89 kDa and an optimum pH of 7.5, whereas VhNag2 has a molecular mass of 73 kDa and an optimum pH of 7.0. …”
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    Novel β-N-acetylglucosaminidases from Vibrio harveyi 650: Cloning, expression, enzymatic properties, and subsite identification
  20. 2500
    por JunGang, Li, KeGui, Zhang, WenJun, Han
    Publicado 2010
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    Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
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