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  1. 2681
    “…We demonstrated in cell culture that the use of the ISA method led to a higher genetic diversity of viral populations than that observed using infectious clone technology. However, no replicative fitness difference was observed. …”
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    Comparison of chikungunya viruses generated using infectious clone or the Infectious Subgenomic Amplicons (ISA) method in Aedes mosquitoes
  2. 2682
    “…Our aim was identify of the pandemic B2-ST131 Escherichia coli clone by to the Institute Pasteur and Achtman scheme, and investigate the resistance profile phenotypic-genotypic, with identification of class 1 integron. …”
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    First Detection of the CTXM-15 Producing Escherichia coli O25-ST131 Pandemic Clone in Ecuador
  3. 2683
    “…Although single-cell cloning is an established method in mammalian cell lines, straightforward protocols are not yet available for insect cells. …”
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    Single-cell cloning enables the selection of more productive Drosophila melanogaster S2 cells for recombinant protein expression
  4. 2684
    “…Asexual reproduction and clonal lineages persist in these taxa despite theoretical arguments suggesting that individual clones should be evolutionarily short-lived due to limited phenotypic diversity. …”
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    Sexual ancestors generated an obligate asexual and globally dispersed clone within the model diatom species Thalassiosira pseudonana
  5. 2685
    “…An intronless endoglucanase from thermotolerant Aspergillus fumigatus DBINU-1 was cloned, characterized and expressed in the yeast Kluyveromyces lactis. …”
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    Cloning and expression of an endoglucanase gene from the thermotolerant fungus Aspergillus fumigatus DBiNU-1 in Kluyveromyces lactis
  6. 2686
    “…The aim of this study was to estimate the level of genetic diversity within this market class potato, utilizing clones and varieties from various breeding programs across the United States. …”
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    Evaluation of genetic diversity among Russet potato clones and varieties from breeding programs across the United States
  7. 2687
    por Yan, Qiang, Fong, Stephen S.
    Publicado 2018
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    Cloning and characterization of a chitinase from Thermobifida fusca reveals Tfu_0580 as a thermostable and acidic endochitinase
  8. 2688
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    Molecular Cloning and Expression Analysis of Lactate Dehydrogenase from the Oriental River Prawn Macrobrachium nipponense in Response to Hypoxia
  9. 2689
    “…BACKGROUND: Direct molecular cloning of full-length cDNAs derived from viral RNA is an approach to identify the individual viral genomes within a virus population. …”
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    Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
  10. 2690
    “…Additionally, the efficiencies of cloning have remained low since the successful production of cloned animals, especially mice. …”
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    Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
  11. 2691
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    Deep Sequencing of Fosmid Clones Indicates Gene Conversion in the Male-Specific Region of the Giant Panda Y Chromosome
  12. 2692
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    Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)
  13. 2693
    “…The histone deacetylase inhibitor (HDACi) has been investigated for treating cancers and many other diseases as well as enhancing the reprogramming efficiency in cloned embryos for decades. In the present study, we investigated the effects of two novel HDAC inhibitors, i.e., HDACi-14 and -79, at the concentrations of 0, 1, 2, or 4 μM on the development of embryos cloned by the oocyte bisection cloning technique (OBCT). …”
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    Novel histone deacetylase inhibitors and embryo aggregation enhance cloned embryo development and ES cell derivation in pigs
  14. 2694
    “…Clinical symptoms of cases infected with sublineage IIb stx2c and stx-negative clones were comparable, despite the loss of stx2c. …”
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    Highly Pathogenic Clone of Shiga Toxin–Producing Escherichia coli O157:H7, England and Wales
  15. 2695
    “…Endoglucanase B (EGLB) derived from Aspergillus niger BCRC31494 has been used in the food fermentation industry because of its thermal and alkaline tolerance. It was cloned and expressed in Pichia pastoris. According to sequence analysis, the gene open reading frame comprises 1,217 bp with five introns (GenBank GQ292753). …”
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    Cloning, Purification, and Characterization of a Heat- and Alkaline-Stable Endoglucanase B from Aspergillus niger BCRC31494
  16. 2696
    “…These resistant strains may belong to a clone of M. tuberculosis that is circulating in the highlands of Ethiopia. …”
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    Line-probe assay and molecular typing reveal a potential drug resistant clone of Mycobacterium tuberculosis in Ethiopia
  17. 2697
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    powerTCR: A model-based approach to comparative analysis of the clone size distribution of the T cell receptor repertoire
  18. 2698
    por Davidson, Rebecca M.
    Publicado 2018
    “…Moreover, the clones have been associated with worse clinical outcomes and show increased pathogenicity in macrophage and mouse models. …”
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    A Closer Look at the Genomic Variation of Geographically Diverse Mycobacterium abscessus Clones That Cause Human Infection and Disease
  19. 2699
    “…Conclusion: We describe an epidemic, tcdA(-)/tcdB(+), multidrug resistant clone of C. difficile from ribotype 017 associated with a hospital outbreak, providing further evidence that the lack of TcdA does not impair the infectious potential of these strains. …”
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    Genomic Study of a Clostridium difficile Multidrug Resistant Outbreak-Related Clone Reveals Novel Determinants of Resistance
  20. 2700
    “…METHODS: We constructed a full-length swine HEV cDNA clone of genotype 4 (named as pGEM-HEV) by reverse genetics approach. …”
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    Successful infection of BALB/c mice by a swine hepatitis E virus clone constructed with reverse genetics
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