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  1. 2781
    “…IL-2-containing pCMV3-C-GFPspark shuttle vector was transformed in E.coli DH5-alpha. After cloning, the plasmid DNA was extracted and transfected in isolated AM-MSCs, by lipofectamine-2000. …”
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    Cloning and expressing of interleukine 2 in amniotic membrane-derived mesenchymal stem cells, as a potent feeder layer
  2. 2782
    por Sarakinos, Andreas, Lembessis, Alkis
    Publicado 2019
    “…The holographic image of an OptoClone(TM) is characterized by a wide angle of view, full parallax and perspective, good color rendition and ultra-realistic reproduction of the optical properties of the materials of an artefact. …”
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    Color Holography for the Documentation and Dissemination of Cultural Heritage: OptoClones(TM) from Four Museums in Two Countries
  3. 2783
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    Long Non-Coding RNA MALAT1 Contributed to the Proliferation of PNH Clone in Paroxysmal Nocturnal Hemoglobinuria Patients
  4. 2784
    “…Here, an antimicrobial peptide gene, Ctri9594, was cloned and functionally identified from the venom of the scorpion Chaerilus tricostatus. …”
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    Molecular Cloning and Functional Identification of the Antimicrobial Peptide Gene Ctri9594 from the Venom of the Scorpion Chaerilus tricostatus
  5. 2785
    “…Nine isolates fulfilled selection criteria and were cloned by limiting dilution and retested. After cloning, one isolate was excluded for not showing transmission. …”
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    A portfolio of geographically distinct laboratory-adapted Plasmodium falciparum clones with consistent infection rates in Anopheles mosquitoes
  6. 2786
    “…These findings warrant prospective monitoring of high-risk clones, which would otherwise worsen the AMR crises.…”
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    International High-Risk Clones Among Extended-Spectrum β-Lactamase–Producing Escherichia coli in Dhaka, Bangladesh
  7. 2787
    “…Among the nine ‘Karaerik’ clones, Clone 8 had the highest total phenolic content (149 mg GAE/100 g FW) while Clone 3 had the lowest (111 mg GAE/100 g FW). …”
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    Phenolic Composition and Antioxidant Activity of Peel, Pulp and Seed Extracts of Different Clones of the Turkish Grape Cultivar ‘Karaerik’
  8. 2788
    “…With increasing complexity of expression studies and the repertoire of characterized sequences, combinatorial cloning has become a common necessity. Techniques like BioBricks and Golden Gate aim to standardize and speed up the process of cloning large constructs while enabling sharing of resources. …”
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    GoldBricks: an improved cloning strategy that combines features of Golden Gate and BioBricks for better efficiency and usability
  9. 2789
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    Attack of the dark clones the genetics of reproductive and color traits of South African honey bees (Apis mellifera spp.)
  10. 2790
    “…Sequences flanking incA were cloned into the pBBR1MCS-4 vector along with the GFP:CAT cassette from the pSW2-GFP chlamydial shuttle vector. …”
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    A broad-spectrum cloning vector that exists as both an integrated element and a free plasmid in Chlamydia trachomatis
  11. 2791
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    Bilineal evolution of a U2AF1-mutated clone associated with acquisition of distinct secondary mutations
  12. 2792
    “…In this work, a novel PrChSaseAC from Pedobacter rhizosphaerae was successfully cloned, expressed in Escherichia coli. After optimizing the induction, the recombinant PrChSaseAC could be expressed efficiently at 0.1 mM IPTG, 25°C, and 12 h induction. …”
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    A Novel Chondroitin AC Lyase With Broad Substrate Specificity From Pedobacter rhizosphaerae: Cloning, Expression, and Characterization
  13. 2793
    “…Core genome comparisons demonstrated that the 36 chicken isolates differed with maximum of nine nucleotides indicating that the same E. coli clone was responsible for all seven disease outbreaks despite adherence to the all-in-all production principle and rigorous cleaning and disinfection procedures. …”
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    Recurring outbreaks by the same Escherichia coli ST10 clone in a broiler unit during 18 months
  14. 2794
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    Cloning, Functional Characterization and Response to Cadmium Stress of the Thioredoxin-like Protein 1 Gene from Phascolosoma esculenta
  15. 2795
    “…It is associated with its virulence since it participates during the invasion process of the host cells. Therefore, cloning, sequencing, and bioinformatic analysis were used to deepen the understanding of the SAG3 locus genetic diversity of T. gondii in blood samples from feral cats. …”
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    SAG3 Toxoplasma gondii cloning reveals unexpected fivefold infection in the blood of feral cats in the Mexican Caribbean
  16. 2796
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    A Genetic Toolkit for Simultaneous Generation of LexA- and QF-Expressing Clones in Selected Cell Types in Drosophila
  17. 2797
    “…We showed that A1 probably emerged as a clone from within A2 and that, because of ongoing gene flow, hospital isolates currently identified as A2 represent a genetic continuum between A1 and community E faecium. …”
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    The interplay between community and hospital Enterococcus faecium clones within health-care settings: a genomic analysis
  18. 2798
    “…To determine whether dsRNA degradation could contribute to low effectiveness of oral RNAi in N. fulva, we cloned its sole dsRNase gene (NfdsRNase). The deduced amino acid sequence contained a signal peptide and an endonuclease domain. …”
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    Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)
  19. 2799
    “…This study investigates the terpenoid compositions and antibacterial and antioxidant activities of eucalypt leaf oils extracted from four E. urophylla clones and one E. urophylla × E. camaldulensis hybrid clone grown in Thailand. …”
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    Variation in the Antibacterial and Antioxidant Activities of Essential Oils of Five New Eucalyptus urophylla S.T. Blake Clones in Thailand
  20. 2800
    “…The four clones showed variable TW and presence of total % G-glucose in the order Björn > Tora > Jorr > Loden. …”
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    The contribution of G-layer glucose in Salix clones for biofuels: comparative enzymatic and HPLC analysis of stem cross sections
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