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  1. 3041
    “…After four rounds of bio-panning, the conventional colony screening method was performed for comparison. The scFv clones retrieved from NGS analysis included all clones identified by the conventional colony screening method as well as many additional clones. …”
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    Next-generation sequencing enables the discovery of more diverse positive clones from a phage-displayed antibody library
  2. 3042
    “…We reprogrammed CD4+ T cell clones from a patient with Sjögren's syndrome (SS) into iPSCs, which were differentiated into DCs (T-iPS-DCs). …”
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    Functional Analysis of Dendritic Cells Generated from T-iPSCs from CD4+ T Cell Clones of Sjögren's Syndrome
  3. 3043
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    A reference genome for Nicotiana tabacum enables map-based cloning of homeologous loci implicated in nitrogen utilization efficiency
  4. 3044
    “…In a first approach, to ascertain that the cloned transcripts allow the expression of the heterodimeric hormones, ag-FSH has been synthesized in human embryonic kidney 293 (HEK293) cells, preliminarily purified and characterized.…”
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    Molecular cloning and characterization of pirarucu (Arapaima gigas) follicle-stimulating hormone and luteinizing hormone β-subunit cDNAs
  5. 3045
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    Cloning and expression of a Trichinella spiralis putative glutathione S-transferase and its elicited protective immunity against challenge infections
  6. 3046
    por Hana, Sam, Lange, Angela B.
    Publicado 2017
    “…In this paper, we report the cDNA sequences of two GPCRs, RhoprOctβ2-R, and RhoprTyr1-R, have been cloned and functionally characterized from Rhodnius prolixus. …”
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    Cloning and Functional Characterization of Octβ2-Receptor and Tyr1-Receptor in the Chagas Disease Vector, Rhodnius prolixus
  7. 3047
    “…RESULTS: C. diphtheriae strains from Belarus belong to ten sequence types (STs). Two major clones, non-toxigenic ST5 and toxigenic ST8, encompassed 76% of the isolates that are associated with sore throat and diphtheria in patients, respectively. …”
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    Genomic analysis of endemic clones of toxigenic and non-toxigenic Corynebacterium diphtheriae in Belarus during and after the major epidemic in 1990s
  8. 3048
    por Fuzi, Miklos, Szabo, Dora, Csercsik, Rita
    Publicado 2017
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    Double-Serine Fluoroquinolone Resistance Mutations Advance Major International Clones and Lineages of Various Multi-Drug Resistant Bacteria
  9. 3049
    “…Despite having a recessive condition, female F8(+/−) cloned pigs died of severe bleeding at an early age, as was the case for male F8(−/Y) cloned pigs, thus making it impossible to obtain progeny. …”
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    Establishment of a strain of haemophilia-A pigs by xenografting of foetal testicular tissue from neonatally moribund cloned pigs
  10. 3050
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    Corrigendum to “Molecular Cloning, Bioinformatic Analysis, and Expression of Bombyx mori Lebocin 5 Gene Related to Beauveria bassiana Infection”
  11. 3051
    “…ROM4 gene was amplified by PCR, cloned into pTG19-T vector and the recombinant plasmid was sequenced. …”
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    Molecular Cloning, Expression and Characterization of Plasmid Encoding Rhomboid 4 (ROM4) of Tachyzoite of Toxoplasma gondii RH Strain
  12. 3052
    “…The procedure consists in the electroporation of intact mouse zygotes with ribonucleoprotein complexes prepared in vitro from recombinant Cas9 nuclease and synthetic dual guide RNA. This simple cloning-free method proves to be extremely efficient for the generation of indels and small deletions by non-homologous end joining, and for the generation of specific point mutations by homology-directed repair. …”
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    Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing
  13. 3053
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    1671 A Fatal Outbreak of a Rare but Emerging Clone of Extensively Drug-resistant Acinetobacter baumannii with Enhanced Virulence
  14. 3054
    “…The genomic DNA sequence (5.6 kb) containing the ckGL gene (designated glp1) was determined and the cDNA was cloned. glp1 was composed of 10 introns and 11 exons, and the 1608-bp full-length cDNA encoded a mature ckGL containing 475 amino acids (aa), with a presequence (60 aa) containing a potential chloroplast transit peptide. …”
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    A novel galactolipase from a green microalga Chlorella kessleri: purification, characterization, molecular cloning, and heterologous expression
  15. 3055
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    Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile
  16. 3056
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    The Pekin duck programmed death ligand-2: cDNA cloning, genomic structure, molecular characterization and expression analysis
  17. 3057
    “…To elucidate mechanisms of tumor progression, we examined the role of interactions between the tumor and its host by using a cloned cell line, ER‐1, which was derived from a rat mammary carcinoma. …”
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    Progression of Weakly Malignant Clone Cells Derived from Rat Mammary Carcinoma by Host Cells Reactive to Plastic Plates
  18. 3058
    “…These findings suggested that the different invasiveness of these clone cells was associated with the difference in the ability of attachment to and degradation of the matrix. …”
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    Effects of Epidermal Growth Factor on Invasiveness through the Extracellular Matrix in High‐ and Low‐metastatic Clones of RCT Sarcoma in vitro
  19. 3059
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    A Novel Gene “Niban” Upregulated in Renal Carcinogenesis: Cloning by the cDNA‐amplified Fragment Length Polymorphism Approach
  20. 3060
    “…Sequence analysis of a cDNA for the rat PSA‐like protein, cloned by immunoscreening of an expression cDNA library prepared from the ventral lobe, revealed identity to the rat submaxillary gland S3 kallikrein. …”
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    Detection and Cloning of a Protein Recognized by Anti‐human Prostate‐specific Antigen (PSA) Antibody in the Rat Ventral Prostate
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