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  1. 3061
    “…RESULTS: The transcriptomic responses of clone G to acute heat stress (from 20 °C to 30 °C) and temperature acclimation (10 °C, 20 °C, and 24 °C) and the proteomic responses of both clones to acute heat, starvation, and heat-and-starvation stress comprised environment-specific and clone-specific elements. …”
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    The transcriptomic and proteomic responses of Daphnia pulex to changes in temperature and food supply comprise environment-specific and clone-specific elements
  2. 3062
    por Nouri, Nima, Kleinstein, Steven H
    Publicado 2018
    “…The ability to quickly and accurately identify members of a clone from repertoire sequencing data will greatly improve downstream analyses. …”
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    A spectral clustering-based method for identifying clones from high-throughput B cell repertoire sequencing data
  3. 3063
    “…The genomes were compared in order to assess the presence of a predominant clone or sequence type (ST). No clonal structure was identified, but core genome-based single nucleotide polymorphism (SNP) analysis distinguished two major, previously identified phylogenetic groups. …”
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    Comparative Genomics of Nonoutbreak Pseudomonas aeruginosa Strains Underlines Genome Plasticity and Geographic Relatedness of the Global Clone ST235
  4. 3064
    “…The biosynthetic gene cluster for bisucaberin B (1, bsb gene cluster), an N-hydroxy-N-succinyl diamine (HSD)-based siderophore, was cloned from the marine bacterium Tenacibaculum mesophilum, originated from a marine sponge. …”
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    Cloning of the Bisucaberin B Biosynthetic Gene Cluster from the Marine Bacterium Tenacibaculum mesophilum, and Heterologous Production of Bisucaberin B
  5. 3065
    “…The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. …”
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    Cloning and high-level expression of Thermus thermophilus RecA in E. coli: purification and novel use in HBV diagnostics
  6. 3066
    “…RESULTS: A chitinase gene from Microbulbifer thermotolerans DAU221 (mtch509) was cloned and expressed in Escherichia coli BL21 (DE3). …”
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    Cloning, purification, and characterization of an organic solvent-tolerant chitinase, MtCh509, from Microbulbifer thermotolerans DAU221
  7. 3067
    por Ataya, Farid Shokry
    Publicado 2012
    “…It is characterized by its capacity to withstand acidic conditions while maintaining high lipolytic activity. We cloned for the first time the full coding sequence of camel’s lysosomal acid lipase, cLIPA using RT-PCR technique (Genbank accession numbers JF803951 and AEG75815, for the nucleotide and aminoacid sequences respectively). …”
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    Cloning, Phylogenetic Analysis and 3D Modeling of a Putative Lysosomal Acid Lipase from the Camel, Camelus dromedarius
  8. 3068
    “…In this research, three vacuolar invertase genes, named MeVINV1-3, and with 653, 660 and 639 amino acids, respectively, were cloned from cassava. The motifs of NDPNG (β-fructosidase motif), RDP and WECVD, which are conserved and essential for catalytic activity in the vacuolar invertase family, were found in MeVINV1 and MeVINV2. …”
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    Cloning, 3D Modeling and Expression Analysis of Three Vacuolar Invertase Genes from Cassava (Manihot Esculenta Crantz)
  9. 3069
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    The molecular cloning of glial fibrillary acidic protein in Gekko japonicus and its expression changes after spinal cord transection
  10. 3070
    por Chen, Cuiping, Xiao, Lu, Li, Xin, Du, Dezhi
    Publicado 2018
    “…Moreover, the candidate gene BjuB07.CLV1 (equivalent to Bjln2) was cloned by comparing mapping and map-based cloning, and BjuB07.CLV1 gene was shown to have the ability to restore the bilocular traits in a genetic complementation experiment. …”
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    Comparative Mapping Combined With Map-Based Cloning of the Brassica juncea Genome Reveals a Candidate Gene for Multilocular Rapeseed
  11. 3071
    “…Heteroresistance to fluconazole (FLC) in Cryptococcus is a transient adaptive resistance which is lost upon release from the drug pressure. It is known that clones heteroresistant to FLC invariably contain disomic chromosomes, but how disomy is formed remains unclear. …”
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    Cryptococcus neoformans, Unlike Candida albicans, Forms Aneuploid Clones Directly from Uninucleated Cells under Fluconazole Stress
  12. 3072
    “…Total fatty acid contents and the degree of unsaturation of total fatty acids increased after the kr or dh gene was knocked in. The cloning and functional study of the pks3 gene of A. limacinum establishes a foundation for revealing the DHA synthetic pathway. …”
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    Cloning of the pks3 gene of Aurantiochytrium limacinum and functional study of the 3-ketoacyl-ACP reductase and dehydratase enzyme domains
  13. 3073
    “…Flavonoid gene expression reveals ANR (1.66%) and F3H (1.02%) were up regulated in the selected P/11/15 clone. The results obtained suggest that P/11/15 clone showed adequate enzyme levels, thus an increased antioxidant activity.…”
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    Biochemical and molecular analysis of Camellia sinensis (L.) O. Kuntze tea from the selected P/11/15 clone
  14. 3074
    por Ahn, Yong Ho
    Publicado 2018
    “…My professional journey to understand the glucose homeostasis began in the 1990s, starting from cloning of the promoter region of glucose transporter type 2 (GLUT2) gene that led us to establish research foundation of my group. …”
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    A Journey to Understand Glucose Homeostasis: Starting from Rat Glucose Transporter Type 2 Promoter Cloning to Hyperglycemia
  15. 3075
    “…It was shown that other tumor clones might contribute more to the resistance to 1(st) EGFR TKIs treatment than tumor clones carrying the EGFR p.T790M mutation when resistance developed. …”
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    Competitive evolution of NSCLC tumor clones and the drug resistance mechanism of first-generation EGFR-TKIs in Chinese NSCLC patients
  16. 3076
    por Haghir, Shahrzad, Alemzadeh, Abbas
    Publicado 2018
    “…Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Triticum turgidum L.) and called TaERF6. …”
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    Cloning and molecular characterization of TaERF6, a gene encoding a bread wheat ethylene response factor
  17. 3077
    por Li, Xin, Hataya, Tatsuji
    Publicado 2019
    “…BACKGROUND: Infectious cDNA clones are a powerful tool for studies on RNA viruses using reverse genetics. …”
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    Construction and characterization of an infectious cDNA clone of potato virus S developed from selected populations that survived genetic bottlenecks
  18. 3078
    “…In addition, miR-125a increases clone longevity, clone size and clonal contribution to hematopoiesis. …”
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    MiR-125a enhances self-renewal, lifespan, and migration of murine hematopoietic stem and progenitor cell clones
  19. 3079
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    Molecular cloning and subcellular localization of six HDACs and their roles in response to salt and drought stress in kenaf (Hibiscus cannabinus L.)
  20. 3080
    “…An outbreak with a remarkable Listeria monocytogenes clone causing 163 cases of non-invasive listeriosis occurred in Germany in 2015. …”
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    A Listeria monocytogenes ST2 clone lacking chitinase ChiB from an outbreak of non-invasive gastroenteritis
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