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  1. 3181
    “…The present study was conducted to characterize the biological function and expression pattern of the HSL gene in the hypothalamic pituitary gonadal (HPG) axis and the development of testis in Bactrian camels. We analyzed cloning of the cDNA sequence of the HSL gene of Bactrian camels by RT-PCR, as well as the structural features of HSL proteins, using bioinformatics software, such as ProtParam, TMHMM, Signal P 4.1, SOPMA and MEGA 7.0. …”
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    Cloning and Molecular Characterization of HSL and Its Expression Pattern in HPG Axis and Testis during Different Stages in Bactrian Camel
  2. 3182
    “…Strains from the outbreak were metallo-β-lactamases and phylogenetically related to the high-risk clone ST233.…”
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    New Variants of Pseudomonas aeruginosa High-Risk Clone ST233 Associated with an Outbreak in a Mexican Paediatric Hospital
  3. 3183
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    Evolution of Chlorhexidine Susceptibility and of the EfrEF Operon among Enterococcus faecalis from Diverse Environments, Clones, and Time Spans
  4. 3184
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    Pathogenomes and variations in Shiga toxin production among geographically distinct clones of Escherichia coli O113:H21
  5. 3185
    “…Here, the putative β-AS gene BcBAS1(Accession No.ON890382) selected according to metabolomic and transcriptomic analyses was cloned and functionally characterized by heterologous expression in Escherichia coli and Pichia pastoris, and its subcellular localization and expression patterns were examined. …”
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    Molecular cloning, functional characterization and expression of the β-amyrin synthase gene involved in saikosaponin biosynthesis in Bupleurum chinense DC.
  6. 3186
    “…Using TGSII‐UNiE, we achieved efficient cloning of long DNA fragments of different sizes and assembly of multiple gene cassettes. …”
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    Efficient assembly of long DNA fragments and multiple genes with improved nickase‐based cloning and Cre/ loxP recombination
  7. 3187
    “…Here, we generated and characterized an eukaryotic-activated full-length infectious cDNA clone for a DENV serotype 1 (DENV-1) isolate, D19044, collected in 2019. …”
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    Development and Characterization of a Genetically Stable Infectious Clone for a Genotype I Isolate of Dengue Virus Serotype 1
  8. 3188
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    Molecular Cloning and Characterization of a Novel Exo-β-1,3-Galactanase from Penicillium oxalicum sp. 68
  9. 3189
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    One clone to rule them all: Culture-independent genomics of Chlamydia psittaci from equine and avian hosts in Australia
  10. 3190
    “…In this study, NoMYB60 was cloned from watercress and its structure and function were analyzed. …”
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    Cloning and Functional Analysis of NoMYB60 Gene Involved in Flavonoid Biosynthesis in Watercress (Nasturtium officinale R. Br.)
  11. 3191
    “…The TreS gene from P. monteilii TBRC 1196 was first cloned and expressed in Escherichia coli. Purified recombinant trehalose synthase (PmTreS) had a molecular weight of 76 kDa and showed optimal pH and temperature at 9.0 and 40°C, respectively. …”
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    Screening, Cloning, Expression and Characterization of New Alkaline Trehalose Synthase from Pseudomonas monteilii and Its Application for Trehalose Production
  12. 3192
    “…Therefore, to effectively clone an antigen-specific TCR from these T cells, we developed a TCR cloning system that does not require a single cell level. …”
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    Rapid identification of CMV-specific TCRs via reverse TCR cloning system based on bulk TCR repertoire data
  13. 3193
    “…To investigate the physiochemical characteristics of two new clones, Salix matsudana ‘J172’ (A7) and Salix matsudana ‘Yankang1’ (A64) in combined Cd-Pb contaminated systems, a hydroponic experiment was designed. …”
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    Metal tolerance capacity and antioxidant responses of new Salix spp. clones in a combined Cd-Pb polluted system
  14. 3194
    “…The objective of this study was to clone a CHS gene from trifoliate orange (Poncirus trifoliata) and analyze its biological information and partial functions. …”
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    Cloning of a CHS gene of Poncirus trifoliata and its expression in response to soil water deficit and arbuscular mycorrhizal fungi
  15. 3195
    “…We cloned the CDS of TaeRF1, which is 1311 bp in length and encodes 436 amino acids. …”
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    Cloning of TaeRF1 gene from Caucasian clover and its functional analysis responding to low-temperature stress
  16. 3196
    “…This study applies portable X-ray fluorescence (pXRF) spectrometry to (i) distinguish Eucalyptus clones using pre-processed pXRF data; and (ii) predict the contents of eleven nutrients in the leaves of Eucalyptus (B, Ca, Cu, Fe, K, Mg, Mn, N, P, S, and Zn) aiming to accelerate the diagnosis of nutrient deficiency. …”
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    A Proximal Sensor-Based Approach for Clean, Fast, and Accurate Assessment of the Eucalyptus spp. Nutritional Status and Differentiation of Clones
  17. 3197
    “…Clone BB-35 recorded the highest (18.8 kg plot(−1)) yield in June, followed by clones 11/4 (18.3) in May, 11/56 (14.7) in November, 6/8 (11.7) in December, and 12/38 (5.78 kg plot(−1)) in June. …”
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    Effect of seasonal variation on yield and leaf quality of tea clone (Camellia sinensis (L.) O. Kuntze) in South West Ethiopia
  18. 3198
    “…Yet, most of the infectious clones of RNA virus constructed in E. coli are unstable and toxic. …”
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    Generation of a Triple-Shuttling Vector and the Application in Plant Plus-Strand RNA Virus Infectious cDNA Clone Construction
  19. 3199
    “…In this study, we demonstrate the application of circular polymerase extension cloning (CPEC) to the generation of full-length clones of SARS-CoV-2. …”
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    A Rapid Method for Generating Infectious SARS-CoV-2 and Variants Using Mutagenesis and Circular Polymerase Extension Cloning
  20. 3200
    “…The cell-culture-generated viruses (passage 12; p12) grew efficiently in human cell lines, but the replication was not fully supported in porcine cells. A full-length cDNA clone was constructed using JE04-1601S_p12 as a template. …”
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    Development and Characterization of Efficient Cell Culture Systems for Genotype 1 Hepatitis E Virus and Its Infectious cDNA Clone
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