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  1. 3281
    “…Cloned pigs presented a more different pattern of DNA methylation in unique sequences in both tissues. …”
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    Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls
  2. 3282
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    Molecular cloning, expression and biochemical characterisation of a cold-adapted novel recombinant chitinase from Glaciozyma antarctica PI12
  3. 3283
    por Chinnaraj, Senniappan, Malimuthu, C
    Publicado 2011
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    Development of micro-propagation and mini cutting protocol for fast growing Melia, Dalbergia and Eucalyptus clones for pulpwood and bio-energy plantations
  4. 3284
    “…However, owing to the lack of a BGal_small_N domain, the domain characteristic for the LacZ enzymes of the GH2 family, it was decided to call the enzyme under study 'BgaL'. The bgaL gene was cloned and expressed in Escherichia coli using the pBAD Expression System. …”
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    A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
  5. 3285
    “…A methicillin-resistant Staphylococcus aureus (MRSA) clone known as ST398 has emerged as a major cause of acute infections in individuals who have close contact with livestock. …”
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    Identification of a Highly Transmissible Animal-Independent Staphylococcus aureus ST398 Clone with Distinct Genomic and Cell Adhesion Properties
  6. 3286
    por Kimura, Tadashi, Ono, Seigo, Kubo, Tai
    Publicado 2012
    “…The cDNA sequences of about 1500 clones out of 4000 clones were analyzed after selection using several criteria. …”
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    Molecular Cloning and Sequence Analysis of the cDNAs Encoding Toxin-Like Peptides from the Venom Glands of Tarantula Grammostola rosea
  7. 3287
    “…The results provide support for the hypothesis that the clones responded differently to an infection control measure based on the use of topical antiseptics, which was more effective at reducing transmission of endemic clones. …”
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    Quantifying Type-Specific Reproduction Numbers for Nosocomial Pathogens: Evidence for Heightened Transmission of an Asian Sequence Type 239 MRSA Clone
  8. 3288
    “…Ribavirin enhanced proliferation of T effector cells and increased production of IFN-gamma in TH1 clones, but had only little effect on IL-10 secretion in TH2 clones. …”
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    Ribavirin Exerts Differential Effects on Functions of Cd4(+) Th1, Th2, and Regulatory T Cell Clones in Hepatitis C
  9. 3289
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    Multi-Locus Variable Number of Tandem Repeat Analysis for Rapid and Accurate Typing of Virulent Multidrug Resistant Escherichia coli Clones
  10. 3290
    “…This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype ‘Gifu’ was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. …”
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    A Set of Lotus japonicus Gifu × Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping
  11. 3291
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    Anti-gp41 antibodies inhibit infection and transcytosis of HIV-1 infectious molecular clones expressing transmitted/founder envelopes
  12. 3292
    “…RESULTS: We report here the cloning of an aryl-alcohol dehydrogenase cDNA from the white-rot fungus Phanerochaete chrysosporium, its expression in Escherichia coli and the biochemical characterization of the encoded GST and His(6) tagged protein. …”
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    Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767
  13. 3293
    “…In the present study, we examined the influence of DNA methylation on transformation efficiency in LAB and developed a direct cloning approach for Lactobacillus plantarum CD033. …”
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    “Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”
  14. 3294
    “…BACKGROUND: Molecular cloning of DNA fragments >5 kbp is still a complex task. …”
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    A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments
  15. 3295
    “…The amplified high-molecular-weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. …”
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    A Fosmid Cloning Strategy for Detecting the Widest Possible Spectrum of Microbes from the International Space Station Drinking Water System
  16. 3296
    “…RESULTS: Twenty five clones specifying cellulose activity were identified. …”
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    Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene
  17. 3297
    “…While screening metagenomic library constructed from DNA extracted from the compost-soil in the p18GFP vector, a clone (TSDV-MX1) was detected that exhibited clear zone of xylan hydrolysis on RBB xylan plate. …”
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    Cloning, Expression and Characteristics of a Novel Alkalistable and Thermostable Xylanase Encoding Gene (Mxyl) Retrieved from Compost-Soil Metagenome
  18. 3298
    “…After cell-autonomous and induced differentiation, each iPSC clone exhibited various differentiation properties, which did not correlate with cell-of-origin. …”
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    Genetically Matched Human iPS Cells Reveal that Propensity for Cartilage and Bone Differentiation Differs with Clones, not Cell Type of Origin
  19. 3299
    “…We describe here the cloning and characterization of two paralogs of cytochrome P450 reductase from W. somnifera. …”
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    NADPH-Cytochrome P450 Reductase: Molecular Cloning and Functional Characterization of Two Paralogs from Withania somnifera (L.) Dunal
  20. 3300
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    Genetic Transformation of a Clinical (Genital Tract), Plasmid-Free Isolate of Chlamydia trachomatis: Engineering the Plasmid as a Cloning Vector
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