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  1. 3301
    “…Leymus mollis is highly tolerant to abiotic stresses such as drought and salinity and resistant to various diseases, but the genetic mechanisms controlling its physiological tolerance remain largely unexplored. We identified and cloned an allene oxide cyclase (AOC) gene from L. mollis that was strongly expressed under salt stress. …”
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    Cloning of allene oxide cyclase gene from Leymus mollis and analysis of its expression in wheat–Leymus chromosome addition lines
  2. 3302
    “…The VIM-2-producing multidrug-resistant high-risk clone Pseudomonas aeruginosa sequence type (ST) 175 was isolated in the setting of a large outbreak in Hospital Universitario 12 de Octubre (Spain) from 2007 to 2010. …”
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    Draft Genome Sequence of VIM-2-Producing Multidrug-Resistant Pseudomonas aeruginosa ST175, an Epidemic High-Risk Clone
  3. 3303
    “…Here, we report molecular cloning and immunochemical characterization of a new Japanese cedar (Cryptomeria japonica) pollen allergen (CPA9) homologous to serine protease, which is initially found as a high IgE-binding spot on our two-dimensional (2-D) IgE immunoblotting map. …”
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    Molecular Cloning and Immunochemical Characterization of a New Japanese Cedar Pollen Allergen Homologous to Plant Subtilisin-Like Serine Protease
  4. 3304
    “…A ligation independent cloning (LIC) system has been developed to facilitate the rapid and high-efficiency cloning of genes in a Bombyx mori expression system. …”
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    Construction of New Ligation-Independent Cloning Vectors for the Expression and Purification of Recombinant Proteins in Silkworms Using BmNPV Bacmid System
  5. 3305
    “…Does asymptomatic clonal T-cell large granular lymphocytic proliferation, in our experience, represent either a secondary cancer after auto-SCT or clonal T cell aberration or derive from expansion of coexisting undetected small-sized clone of T cells?…”
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    Small-Sized Clone of T Cells in Multiple Myeloma Patient after Auto-SCT: T-LGL Leukemia Type or Clonal T-Cell Aberration?
  6. 3306
    “…In this report, the human ALCAM promoter was cloned and its transcriptional mechanisms elucidated. …”
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    Cloning of the human activated leukocyte cell adhesion molecule promoter and identification of its tissue-independent transcriptional activation by Sp1
  7. 3307
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    Emergence of Clostridium difficile infection in tuberculosis patients due to a highly rifampicin-resistant PCR ribotype 046 clone in Poland
  8. 3308
    “…In this study, a JNK from the invasive Mediterranean (MED) species of the whitefly Bemisia tabaci complex was cloned and characterized. The full-length JNK cDNA of MED consists of 1565 bp, with an 1176 bp open reading frame encoding 392 amino acids. …”
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    Cloning and Functional Characterization of c-Jun NH(2)-Terminal Kinase from the Mediterranean Species of the Whitefly Bemisia tabaci Complex
  9. 3309
    “…RESULTS: We present a versatile cloning toolkit for constructing fully-customizable three-part fusion proteins based on the MultiSite Gateway cloning system. …”
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    A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector
  10. 3310
    “…CONCLUSION: In S. furcifera, Sfspo was cloned and the conservation of SfSPO is valid. Thus, SfSPO is probably also involved in ecdysteroidogenesis for hemiptera.…”
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    Molecular cloning and RNA interference-mediated functional characterization of a Halloween gene spook in the white-backed planthopper Sogatella furcifera
  11. 3311
    “…Two of the isolated clones were epidemic and known to disseminate carbapenemases. …”
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    High Proportion of Intestinal Colonization with Successful Epidemic Clones of ESBL-Producing Enterobacteriaceae in a Neonatal Intensive Care Unit in Ecuador
  12. 3312
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    Correction: Positional Cloning Reveals Strain-Dependent Expression of Trim16 to Alter Susceptibility to Bleomycin-Induced Pulmonary Fibrosis in Mice
  13. 3313
    “…The MGL3996 gene encodes an enzyme of 616 amino acids, which shows strong similarity with known CYP52s of other species. MGL3996 gene was cloned and expressed in Pichia pastoris (P. pastoris) heterologous yeast expression system. …”
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    Cloning and Expression in Pichia pastoris of a New Cytochrome P450 Gene from a Dandruff-causing Malassezia globosa
  14. 3314
    “…Flat wart samples on the face from 36 immunocompetent patients were collected and screened for HPV. From one sample, we cloned a putative novel genotype. The novel type consisted of 7779 bp in length with a GC content of 47.1%, containing open reading frames for putative early proteins (E1, E2, E4, E6, and E7) and two late proteins (L1 and L2). …”
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    Molecular Cloning and Characterisation of a Novel Type of Human Papillomavirus 160 Isolated from a Flat Wart of an Immunocompetent Patient
  15. 3315
    “…In this study, we report a rapid cloning strategy for large native plasmids via a contig linkage map by BAC libraries. …”
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    Cloning and Analysis of a Large Plasmid pBMB165 from Bacillus thuringiensis Revealed a Novel Plasmid Organization
  16. 3316
    por Stott, D. I., Sims, G. P.
    Publicado 2000
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    Application of ScFv-Phage Display to Analysis of B-Cell Clones Proliferating in the Salivary Glands of a Patient with Sjögren's Syndrome
  17. 3317
    “…Three HPV58 genomes decoded by RCA-seq displayed a total of 52 nucleotide substitutions from reference HPV58, which could be verified by long PCR template cloning and sequencing. However, the long PCR template cloning and sequencing led to additional nucleotide substitutions, insertions, and deletions from an authentic HPV58 genome in a clinical sample, which vary from one cloned sequence to another. …”
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    Genome sequencing accuracy by RCA-seq versus long PCR template cloning and sequencing in identification of human papillomavirus type 58
  18. 3318
    “…The relationship between gas exchange and leaf hydraulics was similar in both drought-tolerant and drought-sensitive clones despite their contrasting vulnerability to stem cavitation and stomatal response to soil drying. …”
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    Threshold response of mesophyll CO(2) conductance to leaf hydraulics in highly transpiring hybrid poplar clones exposed to soil drying
  19. 3319
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    Molecular Cloning, Bioinformatics Analysis and Expression of Insulin-Like Growth Factor 2 from Tianzhu White Yak, Bos grunniens
  20. 3320
    “…DNA amplification products were cloned in E. coli Xl(1) blue cells and DNA sequences were compared with Mo(1,2) gene in M. oleifera. …”
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    Identification and cloning of putative water clarification genes of Moringa peregrina (Forssk.) Fiori in E. coli Xl(1) blue cells
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