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  1. 3341
    “…However, a number of problems are encountered when attempts are made to clone flanking sequences in T-DNA tagged mutants. …”
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    Frequent problems and their resolutions by using thermal asymmetric interlaced PCR (TAIL-PCR) to clone genes in Arabidopsis T-DNA tagged mutants
  2. 3342
    “…In this work, we collected a high salt-tolerant grass Leymus chinensis (Trin.) as the object of study, and cloned and sequenced the eIF2A gene from this species. …”
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    Gene cloning of an important eukaryotic translation initiation factor family, eIF2A gene in halophytic Leymus chinensis (Trin.)
  3. 3343
    “…Genome DNA content of all clones was stable and comparable to native plants. …”
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    Ex situ conservation of Ruscus aculeatus L. – ruscogenin biosynthesis, genome-size stability and propagation traits of tissue-cultured clones
  4. 3344
    “…The results showed MDA-MB-231 clone expansion at low dose rate after 48–72 hours post-radiation. …”
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    Enhancement of viability of radiosensitive (PBMC) and resistant (MDA-MB-231) clones in low-dose-rate cobalt-60 radiation therapy
  5. 3345
    “…Reference sequences were obtained by cloning and Sanger sequencing of all loci for nine supplementary individuals. …”
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    Species-Level Phylogeny and Polyploid Relationships in Hordeum (Poaceae) Inferred by Next-Generation Sequencing and In Silico Cloning of Multiple Nuclear Loci
  6. 3346
    por Qian, Cen, Fang, Qi, Wang, Lei, Ye, Gong-Yin
    Publicado 2015
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    Molecular Cloning and Functional Studies of Two Kazal-Type Serine Protease Inhibitors Specifically Expressed by Nasonia vitripennis Venom Apparatus
  7. 3347
    “…Two proteins, SghA and SghR, which were recently identified and characterized as novel bacterial virulence factors regulating the infection of plant hosts by Agrobacterium, were cloned, overexpressed and purified with high yield. …”
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    Cloning, expression, purification and crystallization of a pair of novel virulence factors, SghA and SghR, from Agrobacterium tumefaciens
  8. 3348
    “…Here we show that despite its utility as a cloning strain, DH5α retains sufficient recombinase activity to assemble up to six double-stranded DNA fragments ranging in size from 150 bp to at least 7 kb into plasmids in vivo. …”
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    Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies
  9. 3349
    “…High- and low-REST PC12 clones were employed to investigate the role and the mechanisms of the repressor action. …”
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    Epigenomics of Neural Cells: REST-Induced Down- and Upregulation of Gene Expression in a Two-Clone PC12 Cell Model
  10. 3350
    “…Results showed the presence of an inversion of approximately 200 kb in the Ty-2 region when comparing S. lycopersicum and S. habrochaites. By sequencing a BAC clone from the Ty-2 introgression line, one inversion breakpoint was identified. …”
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    Detection of an inversion in the Ty-2 region between S. lycopersicum and S. habrochaites by a combination of de novo genome assembly and BAC cloning
  11. 3351
    “…The study was performed on clones originating from western France (within the native range of this invasive species) and grown for seven years. …”
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    Phenotypic Plasticity in Reproductive Traits of the Perennial Shrub Ulex europaeus in Response to Shading: A Multi-Year Monitoring of Cultivated Clones
  12. 3352
    “…Here, we focus on wild Solanum species and potato clones with varying degree of resistance against Phytophthora infestans, which causes the devastating late blight disease in potato. …”
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    A novel workflow correlating RNA-seq data to Phythophthora infestans resistance levels in wild Solanum species and potato clones
  13. 3353
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    Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis
  14. 3354
    por Hu, Qing, Guo, Wei, Gao, Yu, Tang, Rong, Li, Dapeng
    Publicado 2015
    “…The full-length cDNAs of amh and dax1 in the hermaphrodite, rice-field eel (Monopterus albus), were cloned and characterized in this study. Multiple sequence alignment revealed Dax1 was well conserved among vertebrates, whereas Amh had a low degree of similarity between different vertebrates. …”
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    Molecular cloning and characterization of amh and dax1 genes and their expression during sex inversion in rice-field eel Monopterus albus
  15. 3355
    por Goszczynski, D. E.
    Publicado 2015
    “…The clones share 76.3, 73.2 and 85.2, 77.6 % nt sequence similarity with corresponding clones constructed in Italy and Israel. …”
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    Brief report of the construction of infectious DNA clones of South African genetic variants of grapevine virus A and grapevine virus B
  16. 3356
    “…METHODS: In this study, we cloned and performed a molecular characterization of the complete sequence of Peking duck TLR3 (duTLR3). …”
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    Molecular cloning of Peking duck Toll-like receptor 3 (duTLR3) gene and its responses to reovirus infection
  17. 3357
    “…Multiply antibiotic-resistant Acinetobacter baumannii isolate D36 was recovered in Australia in 2008 and belongs to a distinct lineage of global clone 1 (GC1). Here, we present the complete 4.13 Mbp genome sequence (chromosome plus 4 plasmids), generated via long read sequencing (PacBio).…”
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    Genome Sequence of Acinetobacter baumannii Strain D36, an Antibiotic-Resistant Isolate from Lineage 2 of Global Clone 1
  18. 3358
    “…We aimed to design the fusion protein from Brucella L7/L12 and truncated Omp31proteins, in silico, clone the fusion in pET28a vector, and express it in Escherichia coli host. …”
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    In silico design, cloning and high level expression of L7/L12-TOmp31 fusion protein of Brucella antigens
  19. 3359
    “…This truncated Ig exclusion (TIE) checkpoint ablates PC clones with ΔV-κLCs production and exacerbated ER stress response. …”
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    A plasma cell differentiation quality control ablates B cell clones with biallelic Ig rearrangements and truncated Ig production
  20. 3360
    “…Moreover, two cDNAs, SmeChi-1 encoding SmeChiA, and SmeChi-2 encoding SmeChiB were cloned. The linker regions of the deduced amino acid sequences of SmeChi-1 and SmeChi-2 (SmeChi-1 and SmeChi-2) are the longest among the fish stomach chitinases. …”
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    Stomach Chitinase from Japanese Sardine Sardinops melanostictus: Purification, Characterization, and Molecular Cloning of Chitinase Isozymes with a Long Linker
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