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  1. 3361
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    Molecular Cloning and Functional Characterization of a Novel (Iso)flavone 4′,7-O-diglucoside Glucosyltransferase from Pueraria lobata
  2. 3362
    “…In Europe, the European clone that belongs to sequence type ST80 is predominant. …”
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    Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013
  3. 3363
    “…Differentiated somatic cells can be reprogrammed into totipotent embryos through somatic cell nuclear transfer. However, most cloned embryos arrest at early stages and the underlying molecular mechanism remains largely unexplored. …”
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    Identification of key factors conquering developmental arrest of somatic cell cloned embryos by combining embryo biopsy and single-cell sequencing
  4. 3364
    “…Furthermore, relative miRNAs abundance levels of primary and immortalized human keratinocyte clones were evaluated. The global quantitation of miRNA gene abundance was performed using a TaqMan Low Density Array system. …”
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    Comparison of the miRNA profiles in HPV-positive and HPV-negative tonsillar tumors and a model system of human keratinocyte clones
  5. 3365
    “…To understand the relation between γ-ECS and thiols and to correlate latex flow with one-time tapping and continuous tapping, we cloned and derived the full length of one γ-ECS from rubber tree latex (Hbγ-ECS1). …”
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    Cloning and Expression Analysis of One Gamma-Glutamylcysteine Synthetase Gene (Hbγ-ECS1) in Latex Production in Hevea brasiliensis
  6. 3366
    “…A full-length NtPGIP cDNA of 1,412 bp with a 186 bp 5′-untranslated region (UTR), and 209 bp 3′-UTR was cloned from tobacco, NtPGIP is predicted to encode a protein of 338 amino acids. …”
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    Cloning, expression analysis and recombinant expression of a gene encoding a polygalacturonase-inhibiting protein from tobacco, Nicotiana tabacum
  7. 3367
    “…It shows that CD123 immunoprofiling provides further delineation of FLT3+ LSC clone. This novel finding provides a rationale for treatment involving CD123-targeting antibodies with intracellular FLT3 inhibitors directed against CD34+/CD38−/CD123+. …”
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    Immunoprofiling of leukemic stem cells CD34+/CD38−/CD123+ delineate FLT3/ITD-positive clones
  8. 3368
    “…Using polymerase Chain Reaction (PCR), a novel Δ9- fatty acid desaturase gene was isolated, cloned, and successfully expressed in Escherichia coli. …”
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    Molecular Cloning and Functional Expression of a Δ9- Fatty Acid Desaturase from an Antarctic Pseudomonas sp. A3
  9. 3369
    por Yu, Zhenhua, Li, Ao, Wang, Minghui
    Publicado 2016
    “…We examine CloneCNA on simulated heterogeneous and real tumor samples, and the results demonstrate that CloneCNA has higher power to detect copy number alterations than existing methods. …”
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    CloneCNA: detecting subclonal somatic copy number alterations in heterogeneous tumor samples from whole-exome sequencing data
  10. 3370
    “…RESULTS: Five PLE-positive BAC clones, designated BAC-10, BAC-70, BAC-75, BAC-119 and BAC-206, were identified. …”
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    Third-Generation Sequencing and Analysis of Four Complete Pig Liver Esterase Gene Sequences in Clones Identified by Screening BAC Library
  11. 3371
    “…The evolution of leukemic clones during disease development and treatment may have clinical significance. …”
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    Minimal Residual Disease Detection and Evolved IGH Clones Analysis in Acute B Lymphoblastic Leukemia Using IGH Deep Sequencing
  12. 3372
    “…Regulatory authorities require that cell lines used in commercial production of recombinant proteins must be derived from a single cell progenitor or clone. The limiting dilution method of cell cloning required multiple rounds of low‐density cell plating and microscopic observation of a single cell in order to provide evidence of monoclonality. …”
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    Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high resolution cell imaging
  13. 3373
    “…DISCUSSION: Hair follicles represent a valuable keratinocytes source for in vitro hair cloning for use in treating hair balding or grafting in burn patients. …”
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    Induced pluripotent stem cells from human hair follicle keratinocytes as a potential source for in vitro hair follicle cloning
  14. 3374
    “…In the present study, the Per a 9 gene was cloned and expressed in Escherichia coli (E. coli) systems. …”
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    Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach
  15. 3375
    “…Our aim was to identify the sea lamprey GABA(B1) and GABA(B2) transcripts and study their expression in the CNS of adults. We cloned two partial sequences corresponding to the GABA(B1) and GABA(B2) cDNAs of the sea lamprey as confirmed by sequence analysis and comparison with known GABA(B) sequences of other vertebrates. …”
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    Cloning of the GABA(B) Receptor Subunits B1 and B2 and their Expression in the Central Nervous System of the Adult Sea Lamprey
  16. 3376
    “…To identify components of the transduction pathway, we cloned and analysed the expression pattern of several mRNAs accumulating in cucumber plants after induction of Systemic Acquired Resistance. …”
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    Cloning and expression analysis of cDNAs corresponding to genes activated in cucumber showing systemic acquired resistance after BTH treatment
  17. 3377
    “…A gene cluster involving in methy-oxidation of MDE was cloned from QY7-2 and expressed in Escherichia coli BL21 (DE3), and the products were purified by SDS-PAGE. …”
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    Biodegradation of 3-methyldiphenylether (MDE) by Hydrogenophaga atypical strain QY7-2 and cloning of the methy-oxidation gene mdeABCD
  18. 3378
    “…Our approach utilizes standard cloning techniques while simultaneously performing genome editing in the cloned zygotes of a large animal model for agriculture and biomedical applications.…”
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    Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs
  19. 3379
    “…RESULTS: In this work, the Vibrio cholerae MARTX toxin Cysteine Protease Domain (CPD) was inserted in a ligation-independent cloning (LIC) vector to create a C-terminal 6xHis-tagged inducible autoprocessing enzyme tag, called “the CPD-tag”. …”
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    New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag
  20. 3380
    “…METHODS: A full-length cDNA clone was generated from the DEN-2 virus and used to produce recombinant DEN-2 (rDEN-2) and rDEN2Δ30. …”
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    Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2
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