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  1. 3601
    Publicado 1994
    “…A novel member of the cadherin family of cell adhesion molecules has been characterized by cloning from rat liver, sequencing of the corresponding cDNA, and functional analysis after heterologous expression in nonadhesive S2 cells. cDNA clones were isolated using a polyclonal antibody inhibiting Ca(2+)-dependent intercellular adhesion of hepatoma cells. …”
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    Liver-intestine cadherin: molecular cloning and characterization of a novel Ca(2+)-dependent cell adhesion molecule expressed in liver and intestine
  2. 3602
    “…RESULTS: Using this strategy, we isolated 8 full-length molecular clones from the donor. Two of the eight molecular clones, 03In94_D17 and 03In94_D24, (D17 and D24) generated replication-competent viruses. …”
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    Exceptional molecular and coreceptor-requirement properties of molecular clones isolated from an Human Immunodeficiency Virus Type-1 subtype C infection
  3. 3603
    “…In a strategy aimed at identifying novel markers of human prostate cancer, we performed expression analysis using microarrays of clones randomly selected from a cDNA library prepared from the LNCaP prostate cancer cell line. …”
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    Expression analysis onto microarrays of randomly selected cDNA clones highlights HOXB13 as a marker of human prostate cancer
  4. 3604
    “…RESULTS: The mature peptide sequences of Sl alpha, Sl beta and slr are highly similar to previously cloned salmonid forms and contained the typical motifs. …”
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    Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon: Seasonal expression profile in pituitary and ovary of maturing female broodstock
  5. 3605
    “…We obtained a total of 39 936 soybean cDNA clones (GMFL01 and GMFL02 clone sets) in a full-length-enriched cDNA library which was constructed from soybean plants that were grown under various developmental and environmental conditions. …”
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    Sequencing and Analysis of Approximately 40 000 Soybean cDNA Clones from a Full-Length-Enriched cDNA Library
  6. 3606
    por Haviv, Yosef S
    Publicado 2009
    “…CONCLUSION: A 3-step cloning approach can generate a double-targeted, E1B55k-deleted CRAd using a straight-forward, modified in vitro ligation procedure.…”
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    A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus
  7. 3607
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    Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
  8. 3608
    “…FINDINGS: Here, we characterized the 5' untranslated regions of the different transcript variants. We also cloned and functionally tested the alternatively utilized gene promoters that contribute to the production of different MTUS1 transcript variants. …”
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    Genomic characterization of the human mitochondrial tumor suppressor gene 1 (MTUS1): 5' cloning and preliminary analysis of the multiple gene promoters
  9. 3609
    “…In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. …”
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    A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus
  10. 3610
    por Ewald, Rebecca C., Cline, Hollis T.
    Publicado 2009
    “…The NR1-4a/b splice variants appear to be the predominant isoforms while the NR1-3a/b variants appear to be expressed at low levels. We cloned the X. laevis NR2A and NR2B subunits and provide a detailed annotation of their functional domains in comparison with NR2A and NR2B proteins from 10 and 13 other species, respectively. …”
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    Cloning and Phylogenetic Analysis of NMDA Receptor Subunits NR1, NR2A and NR2B in Xenopus laevis Tadpoles
  11. 3611
    por Burnell, Jim N
    Publicado 2010
    “…RESULTS: This paper describes the cloning and expression of both PEPS and DUF299 from E. coli and establishes that E. coli DUF299 catalyzes both the ADP-dependent inactivation and the P(i)-dependent activation of PEPS. …”
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    Cloning and characterization of Escherichia coli DUF299: a bifunctional ADP-dependent kinase - P(i)-dependent pyrophosphorylase from bacteria
  12. 3612
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    Induction/Engineering, Detection, Selection, and Expansion of Clinical-Grade Human Antigen-Specific CD8(+) Cytotoxic T Cell Clones for Adoptive Immunotherapy
  13. 3613
    “…Insulin-reactive CD8 T cells are amongst the earliest islet-infiltrating CD8 T cells in NOD mice. Cloned insulin B15–23-reactive cells (designated G9C8), restricted by H-2K(d), are highly diabetogenic. …”
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    Functional inhibition related to structure of a highly potent insulin-specific CD8 T cell clone using altered peptide ligands
  14. 3614
    “…RESULTS: Therefore, a cDNA encoding Eriocheir sinensis FABP (Es-FABP) was cloned based upon EST analysis of a hepatopancreas cDNA library. …”
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    Molecular cloning and tissue expression of the fatty acid-binding protein (Es-FABP) gene in female Chinese mitten crab (Eriocheir sinensis)
  15. 3615
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    Drosophila type II neuroblast lineages keep Prospero levels low to generate large clones that contribute to the adult brain central complex
  16. 3616
    “…In this paper, hsp60 of the stem borer, Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), was cloned by RT-PCR and rapid amplification of cDNA end (RACE) reactions. …”
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    Cloning of the Heat Shock Protein 60 Gene from the Stem Borer, Chilo suppressalis, and Analysis of Expression Characteristics Under Heat Stress
  17. 3617
    “…PxylCSP3 and Pxyl-CSP4, with GenBank accession numbers ABM92663 and ABM92664, respectively, were cloned and sequenced. The gene sequences both consisted of three exons and two introns. …”
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    Molecular Cloning, Expression Profile and 5′ Regulatory Region Analysis of Two Chemosensory Protein Genes from the Diamondback Moth, Plutella xylostella
  18. 3618
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    Cloning and Characterization of an Armillaria gallica cDNA Encoding Protoilludene Synthase, Which Catalyzes the First Committed Step in the Synthesis of Antimicrobial Melleolides
  19. 3619
    por Jang, Goo, Hong, So Gun, Lee, Byeong Chun
    Publicado 2011
    “…DNA analysis showed that all cloned calves were genetically identical to the donor cells. …”
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    Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
  20. 3620
    “…The aims of this study were to clone the bovine NPM2 gene, determine its temporal expression during oocyte development and early embryogenesis, and evaluate the potential role of miRNA-181a in regulation of its expression. …”
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    Molecular cloning and expression of bovine nucleoplasmin 2 (NPM2): a maternal effect gene regulated by miR-181a
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