Mostrando 3,721 - 3,740 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 2.42s Limitar resultados
  1. 3721
  2. 3722
    “…In this study, we investigated the prevalence and molecular characteristics of this clone isolated from a Chinese university hospital. …”
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  3. 3723
  4. 3724
  5. 3725
    “…The exponentially increasing volumes of DNA sequence data highlight the need for new DNA cloning methods to explore the new information. Here, we describe ‘ExoCET’ (Exonuclease Combined with RecET recombination) to directly clone any chosen region from bacterial and mammalian genomes with nucleotide precision into operational plasmids. …”
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  6. 3726
  7. 3727
    “…MATERIALS AND METHODS: We performed cloning and protein expression of Mce1A gene of Mycobacterium tuberculosis Beijing strain as local isolate and standard strain H37Rv as a comparison on the expression system Escherichia coli BL21(DE3). …”
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  8. 3728
    “…The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v‐Ha‐ras) followed by treatment with a tumor promoter, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), suggesting that the ras oncogene acted as an initiator in two‐stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras‐transfected BALB/3T3 cells. …”
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  9. 3729
    “…To construct A9 cell clones that contain a single X/autosome translocation, micro‐cell fusion was undertaken to transfer these chromosomes from the hybrids to A9 cells. …”
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  10. 3730
  11. 3731
    “…A clone with a high metastatic potential (MH‐02) showed a characteristic biphasic pattern of platelet aggregation, of which the first peak was not present in the aggregation patterns of the clone with low metastatic potential (ML‐01). …”
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  12. 3732
    “…In order to determine whether or not the VHL gene is also a critical gene in rat RCs, we cloned and sequenced the rat homologue of human VHL gene and searched for mutations of the VHL gene in rat RCs. …”
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  13. 3733
    “…We investigated the roles of extracellular matrix proteins, iaminin and fibronectin, in promoting invasiveness through the extracellular matrix in high‐metastatic [RCT(+)] clone cells established from poorly differentiated murine RCT sarcoma in C3H/He mice. …”
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  14. 3734
    “…Among these, the α-β hydrolase enzyme is known for the degradation of organophosphate pesticides. The cloning and expression of the α-β hydrolase gene imply nonspecific cleavage of antibiotics through a cross-resistance phenomenon in the host. …”
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  15. 3735
    “…In this study, the full-length cDNAs of FAD8-1, FAD8-2, and FAD8-3 are cloned from Paeonia ostii T. Hong & J. X. Zhang and named as PoFAD8-1, PoFAD8-2, and PoFAD8-3. …”
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  16. 3736
  17. 3737
    “…In a recent clone-tracking experiment, millions of uniquely tagged hematopoietic stem cells (HSCs) and progenitor cells were autologously transplanted into rhesus macaques and peripheral blood containing thousands of tags were sampled and sequenced over 14 years to quantify the abundance of hundreds to thousands of tags or “clones.” …”
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  18. 3738
    “…Abnormalities in gene expression that negatively affect embryonic development are frequently observed in cloned embryos generated by somatic cell nuclear transfer (SCNT). …”
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  19. 3739
    “…A total of 267 small ruminants (220 sheep, 47 goats) were included; half received RVFV Clone 13 vaccine at the recommended dose and half received the diluent (as placebo) only. …”
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  20. 3740
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