Mostrando 361 - 380 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.21s Limitar resultados
  1. 361
    “…MATERIALS AND METHODS: The S. simulans gene encoding lysostaphin was extracted, amplified by polymerase chain reaction (PCR), and sub-cloned in prokaryotic expression vector pET32a. E. coli BL21 (DE3) plysS were transformed with pET32a-lys and gene expression was induced by IPTG. …”
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  2. 362
    por Itoh, Keiji, Sokol, Sergei Y.
    Publicado 2014
    “…Here we use an expression cloning approach to identify variable regions of llama single domain antibodies (known as nanobodies), which recognize specific embryonic antigens. …”
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  3. 363
    “…However, due to the genetic makeup of plastids and the distinct features of the transformation process, vector design, and the use of specific genetic elements, a large set of basic transformation vectors is required, making cloning a tedious and time-consuming effort. Here, we describe the adoption of standardized modular cloning (GoldenBraid) to the design and assembly of the full spectrum of plastid transformation vectors. …”
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  8. 368
    “…Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. …”
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  9. 369
    “…The first biologically active infectious clones of tomato torrado virus (ToTV) were generated and delivered into Nicotiana benthamiana and Solanum lycopersicum plants via Agrobacterium tumefaciens. …”
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  10. 370
    por Jacobus, Ana Paula, Gross, Jeferson
    Publicado 2015
    “…We tested these optimized cloning parameters during the assembly of five independent DNA constructs and obtained 94% positive clones out of 100 colonies probed. …”
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  11. 371
    “…This report describes the cloning and characterization of the human ITGB6 promoter. …”
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  12. 372
    “…The phbCAB operon was amplified using designed primers. The fragment was cloned into pET-28a expression vector and then transformed into Escherichia coli BL21. …”
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  13. 373
    “…In this study, we characterized three Eimeria tenella cDNA clones encoding LPP named EtLPP1, EtLPP2 and EtLPP3. …”
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  14. 374
    “…BACKGROUND: With the development of several new technologies using synthetic biology, it is possible to engineer genetically intractable organisms including Mycoplasma mycoides subspecies capri (Mmc), by cloning the intact bacterial genome in yeast, using the host yeast’s genetic tools to modify the cloned genome, and subsequently transplanting the modified genome into a recipient cell to obtain mutant cells encoded by the modified genome. …”
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  15. 375
    por Zheng, Bei, Chang, Wei-Shan
    Publicado 2014
    “…Sequence the cloning sequences, comparing the sequencing results by NCBI. …”
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  16. 376
    “…In addition, we sequenced the gap between two contigs from the shotgun cloning of the turkey genome, and found the missing sequence for the turkey Sec-tRNA. …”
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