Mostrando 381 - 400 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.39s Limitar resultados
  1. 381
    “…Our results showed that aggregation of three embryos increased blastocyst formation rate and blastocyst diameter of cloned pig embryos. DNA fragmentation levels in 3x aggregated cloned blastocysts were significantly decreased compared to 1x blastocysts. …”
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  2. 382
    “…We report here a PCR-based cloning methodology that requires no post-PCR modifications such as restriction digestion and phosphorylation of the amplified DNA. …”
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  3. 383
    “…However, such epigenetic memories often persist throughout the course of clonal development, thus decreasing cloning efficiency. Here, we explored reprogramming-refractory regions in bovine SCNT blastocyst transcriptomes. …”
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  4. 384
    “…These hospital-associated clones are characterized by the acquisition of adaptive genetic elements, including genes involved in metabolism, biofilm formation, and antibiotic resistance. …”
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  5. 385
    “…According to the distinct pooling pattern for each clone in the overlapping pool sequencing, alleles for the recovered variants could be assigned to their original clones precisely. …”
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  6. 386
    “…Methods: Rabbit Nox5 was amplified from rabbit tissue, cloned, and sequenced. COS-7 cells were employed for expression and functional analysis via Western blotting and measurements of superoxide. …”
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  7. 387
    por Nicholson, Michael D., Antal, Tibor
    Publicado 2016
    “…Deterministically growing (wild-type) populations which seed stochastically developing mutant clones have found an expanding number of applications from microbial populations to cancer. …”
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  8. 388
    “…To date, few studies have investigated whether or not these cloned dogs will show identical behavior patterns. …”
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  9. 389
    “…However, somatic cloning has been inefficient in all species in which live clones have been produced. …”
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  10. 390
  11. 391
    “…We examined the extent of within and between clone variation in gene expression in the earthworm Dendrobaena octaedra, which reproduces through apomictic parthenogenesis. …”
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  12. 392
    “…BACKGROUND: The aim of this study was the application of complementarity-determining region-3 spectratyping analysis to determine T-cell-repertoire complexity and to detect T-cell-clone expansion, as a measure of immune response in nonfunctioning kidney transplants (group hemodialysis-transplant [HD-Tx]), nontransplanted dialysis patients (group hemodialysis [HD]), and normal subjects as controls (group C). …”
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  13. 393
    “…RESULTS: A TcTrx (Tc: Taiwanofungus camphorata) cDNA (640 bp, GenBank AY838902.1) encoding a putative thioredoxin (Trx) of 135 amino acid residues with calculated molecular mass of 16.17 kDa was cloned from Taiwanofungus c amphorata. The deduced amino acid sequence containing a motif (Cys-Gly-Pro-Cys) that is highly conserved among the reported Trxs. …”
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  14. 394
    por Hanke, Moritz, Foraita, Ronja
    Publicado 2017
    “…CONCLUSIONS: We suggest to use clone temporal centrality measures in incomplete graph sequences settings. …”
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  15. 395
    “…Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined.…”
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  16. 396
  17. 397
    “…The study provides insight into the current status of cloning, expression and sequence analysis of β-Xylosidases for industrial applications.…”
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  18. 398
    “…Whole genome sequencing mixes the signals of sampled populations, diluting the signals of clone-specific aberrations, and complicating estimation of clone-specific genotypes. …”
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  19. 399
    “…This result fails to reject the hypothesis of a single hybridization founder event that resulted in the initial formation of one major clone. The other clones appear to have originated via post-formation microsatellite mutations of the major clone.…”
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  20. 400
    “…Cloning was confirmed by colony PCR, double digestion analysis and sequencing. …”
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