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Mostrando 401 - 420 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.31s Limitar resultados
  1. 401
    “…Rather, using mathematical modelling and computational lattice-based simulations, we show that observed clone size distributions can be explained by a combination of neutral drift and stochastic nucleation of mutations at the boundary of expanding mutant clones that have a competitive advantage. …”
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    Spatial constraints govern competition of mutant clones in human epidermis
  2. 402
    “…Pig is a common laboratory model, however, to the best of our knowledge, its RPL21 gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pig RPL21 gene for the first time. …”
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    Molecular cloning and characterization of porcine ribosomal protein L21
  3. 403
    “…Plant virus infectious clones are important tools with wide‐ranging applications in different areas of biology and medicine. …”
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    A guide to the contained use of plant virus infectious clones
  4. 404
    “…Although these clones expressed similar phenotypes to the parent LAK cells, Lyt‐2 was expressed in a restricted portion of the clones. …”
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    Establishment of Mouse Lymphokine‐activated Killer Cell Clones and Their Properties
  5. 405
    “…MATERIAL AND METHODS: Cell division cycle 42 (Cdc42) from sheep (Ovis aries) (OaCdc42) was cloned by rapid amplification of cDNA ends (RACE), and then tissue distribution and differential expression levels of OaCdc42 mRNA between infected and vaccinated sheep were analysed by RT-qPCR. …”
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    Cloning and Differential Expression Analyses of Cdc42 from Sheep
  6. 406
    “…Different approaches have been used to clone aging and longevity-related genes. In this article we review these cloning techniques and discuss their advantages and limitations. …”
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    Cloning Approaches for Identifying Aging and Longevity-Related Genes in Mammals
  7. 407
    “…We report the identification of Pseudomonas strains with CoA-independent acyltransferase activity as well as the heterologous expression of the enzyme in E. coli. The cloning strategies and selected expression studies are discussed. …”
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    Molecular cloning, expression, and characterization of acyltransferase from Pseudomonas protegens
  8. 408
    “…FX cloning allows for the efficient cloning into an entry vector and the facile transfer of the sequenced insert into a variety of destination vectors. …”
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    A uniform cloning platform for mycobacterial genetics and protein production
  9. 409
    “…B cell clones expand and contract during adaptive immune responses and can persist or grow uncontrollably in lymphoproliferative disorders. …”
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    Computational Evaluation of B-Cell Clone Sizes in Bulk Populations
  10. 410
    “…However, due to the repetitive character and the presence of multiple gene copies, high-throughput cloning of TAL effector genes remains a challenge. …”
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    Efficient enrichment cloning of TAL effector genes from Xanthomonas
  11. 411
    “…AIM: The present study aims to investigate the performance of 2 different PCR protocols; real-time quantitative molecular assays (qPCR) and conventional molecular assays (cPCR), using 2 different sets of primers and by using cloned purified Toxoplasma genomic substances to be evaluated as reference samples. …”
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    Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites
  12. 412
    “…Its promoter activity was examined via the dual-luciferase reporter assay after it had been cloned into a pGL(3)-basic vector generating pGL(3)-p2.6kb and transfected into LNCaP cells. pGL(3)-basic and pGL(3)-control were respectively used as the negative and positive controls. …”
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    Molecular cloning and analysis of the human PCAN1 (GDEP) promoter
  13. 413
    “…This study is the first to clone and characterize the AFAP1-AS1 promoter region. …”
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    Cloning and characterization of the putative AFAP1-AS1 promoter region
  14. 414
    por DAL, Elif, ALKAN, Can
    Publicado 2018
    “…Here we evaluate the accuracies of several genome scaffolding algorithms using two different types of data generated from the genome of the same human individual: whole genome shotgun sequencing (WGS) and pooled clone sequencing (PCS). We observe that it is possible to obtain better assemblies if PCS data are used, compared to using only WGS data. …”
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    Evaluation of genome scaffolding tools using pooled clone sequencing
  15. 415
    por Saini, Monika, Selokar, Naresh L.
    Publicado 2018
    “…The reproductive cloning in buffalo in India has been started using a simplified somatic cell nuclear transfer technique named handmade cloning. …”
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    Approaches used to improve epigenetic reprogramming in buffalo cloned embryos
  16. 416
    “…In this study, a modified method of handmade cloning (m-HMC), which had been originally developed in sheep, was used for somatic cell nuclear transfer (SCNT) in the dromedary camel. …”
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    Development of a modified method of handmade cloning in dromedary camel
  17. 417
    “…Consequently, a typical molecular biology laboratory working across kingdoms must use multiple cloning strategies to assemble DNA constructs for experimental assays. …”
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    A unified multi-kingdom Golden Gate cloning platform
  18. 418
    “…Thirty-two samples were obtained from ten patients. PyClone identified 2 to 10 genetic sub-clones per tumor. …”
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    Rectal cancer sub-clones respond differentially to neoadjuvant therapy()
  19. 419
    “…In the present study, we identify and analyze the DNA sequences of Pa-THYs by bioinformatics analysis. Then we clone, express, and purify the Pa-THYs proteins and evaluate the activity of recombinant Pa-THYs proteins by cell migration and proliferation assays in NIH/3T3 cells. …”
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    Cloning, Expression and Effects of P. americana Thymosin on Wound Healing
  20. 420
    “…SIMPLE SUMMARY: Cloning is the most promising technique for passing the excellent phenotypes of the best individuals in the population. …”
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    Study on Hematological and Biochemical Characters of Cloned Duroc Pigs and Their Progeny
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