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  1. 4781
    “…In silico analysis reveals that the isolate belongs to a ST410-H24Rx high-risk clone. It coharbors the ESBL-encoding genes bla(CTX-M-15), bla(TEM-1), bla(OXA-1) and bla(NDM-5) on an IncFIA/IncFIB/IncFII/IncQ1 multireplicon plasmid. …”
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    Genomic Characterization of International High-Risk Clone ST410 Escherichia coli Co-Harboring ESBL-Encoding Genes and bla(NDM-5) on IncFIA/IncFIB/IncFII/IncQ1 Multireplicon Plasmid and Carrying a Chromosome-Borne bla(CMY-2) from Egypt
  2. 4782
    “…In the present article, we reported our experience with the novel anti-p16 INK4a antibody (clone BC42), whose expression was tested across all different gynecologic neoplasms; we also compared it to the traditional E6H4 clone. …”
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    An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls
  3. 4783
    por Browne, Peter E.
    Publicado 2007
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    Beat clón : prosa y poesía del camino /
  4. 4784
    por Cortázar, Julio
    Publicado 1980
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    Queremos tanto a Glenda
  5. 4785
    por Bajpai, Bhakti
    Publicado 2013
    “…Since the construction of the first generation of general cloning vectors in the early 1970s, a large number of cloning vectors have been developed. …”
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    High Capacity Vectors
  6. 4786
    “…Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids. …”
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    Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost
  7. 4787
    “…DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. …”
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    An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
  8. 4788
    “…The results showed a considerable improvement in cloning efficiency and demonstrated the important role of vector ratio in the cloning process. …”
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    The TelN/tos-assisted precise targeting of chromosome segments (TAPE)
  9. 4789
    Tabla de Contenidos: “…Introduction -- Basic molecular biology -- How to clone a gene -- Cutting and joining DNA -- Vectors -- Genomic and cDNA libraries -- Finding the right clone -- Polymerase chain reaction -- Characterization of a cloned gene -- Analysis of gene expression -- Products from native and manipulated cloned genes -- Genomic analysis -- Analysis of genetic variation -- Post-genomic analysis -- Modifying organisms; transgenics.…”
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    From genes to genomes : concepts and applications of DNA technology /
  10. 4790
    por Goate, Alison M.
    Publicado 1995
    “…Two main types of technology are commonly used to identify genes that cause or predispose a person to a disease: positional cloning techniques and candidate gene techniques. …”
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    Molecular Biology
  11. 4791
    “…CONCLUSION: PlasmaDNA is a program available both on Windows and Apple operating systems, designed to facilitate molecular cloning experiments by building a visual map of the DNA. …”
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    PlasmaDNA: a free, cross-platform plasmid manipulation program for molecular biology laboratories
  12. 4792
    “…When serial or successive rounds of cloning are performed, blastocyst and full term development rates decline even further with the increasing rounds of cloning. …”
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    Transcriptional reprogramming of gene expression in bovine somatic cell chromatin transfer embryos
  13. 4793
    “…Based on the final cloning plasmid, pFPAV3-CcdB-Cm, and our modified SLiCE strategy (SLiCE cloning and lethal CcdB screening), the process for generating recombinant PAdV-3 plasmids required only one step in 3 days, with a cloning efficiency as high as 620±49.56 clones/ng and zero background (100% accuracy). …”
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    A Novel and Simple Method for Rapid Generation of Recombinant Porcine Adenoviral Vectors for Transgene Expression
  14. 4794
    “…IMPORTANCE We complemented a cloning platform with new editing plasmids that allow a quick transition from high-throughput cloning and the expression of new enzymes to the stable integration of genes for the production of enzymes through B. subtilis fermentation. …”
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    Fragment Exchange Plasmid Tools for CRISPR/Cas9-Mediated Gene Integration and Protease Production in Bacillus subtilis
  15. 4795
    “…The cloning of plasmids can be time-consuming or expensive. …”
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    Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi
  16. 4796
    “…SOLiD cloning data differ from Illumina cloning data mainly because of distinct methods of adapter ligation. …”
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    Sequencing bias: comparison of different protocols of MicroRNA library construction
  17. 4797
    por Kobori, Shungo, Ko, Yumi, Kato, Mikio
    Publicado 2009
    “…Residual insertion sequence elements (IS elements) in Escherichia coli strains that are commonly used for DNA cloning are known to cause cloning artifacts by transposing themselves into the recombinant DNA fragments. …”
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    A Target Site for Spontaneous Insertion of IS10 Element in pUC19 DNA Located within Intrinsically Bent DNA
  18. 4798
    “…Mammalian oocytes can reprogram differentiated somatic cells into a totipotent state through somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian species have been successfully cloned, the majority of cloned embryos failed to develop to term, resulting in the overall cloning efficiency being still low. …”
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    Strategies to Improve the Efficiency of Somatic Cell Nuclear Transfer
  19. 4799
    “…A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. …”
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    Vectors for co-expression of an unrestricted number of proteins
  20. 4800
    “…InTag positive selection enables recombinant clones of interest to be directly selected without cloning background, bypassing the laborious process of plating out cultures and colony screening and enabling the cloning procedure to be automated and performed in a high-throughput format. …”
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    One-step zero-background IgG reformatting of phage-displayed antibody fragments enabling rapid and high-throughput lead identification
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