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  1. 4801
    “…BACKGROUND: The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. …”
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    A novel prokaryotic vector for identification and selection of recombinants: Direct use of the vector for expression studies in E. coli
  2. 4802
    “…Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly of complex multipart constructs from reusable basic DNA parts in a rapid, reliable and automation-friendly way. …”
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    Joint universal modular plasmids (JUMP): a flexible vector platform for synthetic biology
  3. 4803
    “…[Image: see text] Modern cloning methods are independent from restriction enzyme recognition sites. …”
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    In Situ Overlap and Sequence Synthesis During DNA Assembly
  4. 4804
    “…Pre-cloning and post-cloning susceptibility of the strain was done for assessment of acquired resistance. …”
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    Escherichia coli harbouring strAB with reduced susceptibility towards gentamicin and amikacin: a single centre study from India
  5. 4805
    “…Although traditional cloning techniques using restriction enzymes or a site-specific recombination system (Gateway or Clontech cloning technology) are highly useful for efficiently transferring DNA fragments into destination plasmids, the process is time consuming and expensive. …”
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    A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics
  6. 4806
    “…BACKGROUND: Functional genomic research always needs to assemble different DNA fragments into a binary vector, so as to express genes with different tags from various promoters with different levels. The cloning systems available bear similar disadvantages, such as promoters/tags are fixed on a binary vector, which is generally with low cloning efficiency and limited for cloning sites if a novel promoter/tag is in need. …”
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    BioVector, a flexible system for gene specific-expression in plants
  7. 4807
    “…To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning. RSF1010-based plasmids replicate in diverse bacterial strains but produce low amounts of useable DNA for cloning. …”
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    Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting
  8. 4808
    “…BACKGROUND: Widely used restriction-dependent cloning methods are labour-intensive and time-consuming, while several types of ligase-independent cloning approaches have inherent limitations. …”
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    An asymmetric PCR-based, reliable and rapid single-tube native DNA engineering strategy
  9. 4809
    “…BACKGROUND: Restriction-free (RF) cloning, a PCR-based method for the creation of custom DNA plasmids, allows for the insertion of any sequence into any plasmid vector at any desired position, independent of restriction sites and/or ligation. …”
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    A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends
  10. 4810
    “…The method described is most directly compatible with Golden Gate cloning but is, in principle, compatible with any cloning method. …”
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    A qPCR Method to Distinguish between Expression of Transgenic and Endogenous Copies of Genes
  11. 4811
    “…We applied tagmentation to metagenomic DNA from soil and gut microbiomes to prepare DNA inserts for high-throughput cloning into functional metagenomic libraries. The presence of mosaic end sequences in the resulting DNA fragments synergized with homology-based assembly cloning to result in a 300-fold increase in cloning efficiency compared to traditional blunt-cloning-based protocols. …”
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    Mosaic Ends Tagmentation (METa) Assembly for Highly Efficient Construction of Functional Metagenomic Libraries
  12. 4812
    por Itaya, Mitsuhiro, Kaneko, Shinya
    Publicado 2010
    “…The stable cloning of giant DNA is a necessary process in the production of recombinant/synthetic genomes. …”
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    Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method
  13. 4813
    “…While this approach relieved the cellular cloning bottleneck and had the desirable ability to screen antibody function prior to cloning, the small volume of hybridoma supernatant available for screening limited the number of antigens for pooled immunisation. …”
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    Development of an antigen microarray for high throughput monoclonal antibody selection
  14. 4814
    “…The existing molecular cloning methods are often limited by the availability of suitable restriction sites. …”
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    An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution
  15. 4815
    “…Four case studies are presented that demonstrate the use of λ‐PCR in simple gene cloning, simultaneous multipart assemblies, gene cloning not achievable with commercial kits, and the use of thermodynamic simulations to guide λ‐PCR assembly strategies. …”
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    Lambda‐PCR for precise DNA assembly and modification
  16. 4816
    “…BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the genome by ordinary PCR based techniques. …”
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    Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA
  17. 4817
    “…Body Mass Index was significantly negatively correlated with W5C5(+) cloning efficiency and W5C5(−) cloning efficiency (P<0.01, respectively), and positively correlated with first trimester loss (P<0.01). …”
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    Deficiency in Clonogenic Endometrial Mesenchymal Stem Cells in Obese Women with Reproductive Failure – a Pilot Study
  18. 4818
    “…Animal cloning has been achieved in many species by transplanting differentiated cell nuclei to unfertilized oocytes. …”
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    Reprogramming towards totipotency is greatly facilitated by synergistic effects of small molecules
  19. 4819
    “…Animal cloning is of great importance to the production of transgenic and genome-edited livestock. …”
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    TSA Activates Pluripotency Factors in Porcine Recloned Embryos
  20. 4820
    por Nørholm, Morten HH
    Publicado 2010
    “…BACKGROUND: The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences. …”
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    A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering
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