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  1. 4821
    por Anisimov, Sergey V, Sharov, Alexei A
    Publicado 2004
    “…We have employed a mathematical model to predict the frequency of quasi-ditags in random nucleotide sequences, and our data show that clones containing less than or equal to 2 ditags (which include chromosomal cloning artifacts) should be excluded from the analysis of SAGE catalogs. …”
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    Incidence of "quasi-ditags" in catalogs generated by Serial Analysis of Gene Expression (SAGE)
  2. 4822
    “…Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures. …”
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    Development of Versatile Vectors for Heterologous Expression in Bacillus
  3. 4823
    “…With the recent advancements in genome editing, next-generation sequencing (NGS), and scalable cloning techniques, scientists can now conduct genetic screens at unprecedented levels of scale and precision. …”
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    An integrated pipeline for mammalian genetic screening
  4. 4824
    por Strand, Tanja M, Höglund, Jacob
    Publicado 2011
    “…At the individual level however, there was a trend towards more alleles scored with RSCA (1-6 per individual) than cloning/sequencing (1-4 per individual). In 63% of the pair-wise comparison, the identical allele was scored in RSCA as in cloning/sequencing. …”
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    Genotyping of black grouse MHC class II B using reference Strand-Mediated Conformational Analysis (RSCA)
  5. 4825
    “…Based on a considerably large data set from 274 SCNT experiments (in total 18,649 reconstructed embryos transferred into 193 recipients), performed over a period of three years, we assessed the relative contribution of season, type of genetic modification, donor cell source, number of cloning rounds, and pre-selection of cloned embryos for early development to the cloning efficiency. …”
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    Factors influencing the efficiency of generating genetically engineered pigs by nuclear transfer: multi-factorial analysis of a large data set
  6. 4826
    “…Yarrowia lipolytica is an alternative yeast for heterologous protein production. Based on auto-cloning vectors, a set of 18 chromogenic cloning vectors was developed, each containing one of the excisable auxotrophic selective markers URA3ex, LYS5ex, and LEU2ex, and one of six different promoters: the constitutive pTEF, the phase dependent hybrid pHp4d, and the erythritol-inducible promoters from pEYK1 and pEYL1 derivatives. …”
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    Production of Rhizopus oryzae lipase using optimized Yarrowia lipolytica expression system
  7. 4827
    por Fuhrmeister, Robert, Streubel, Jana
    Publicado 2023
    “…Finally, to further simplify the cloning of potential candidate transporters, we provide level 0 modular cloning (MoClo) modules for efficient and simple Golden Gate cloning. …”
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    Functional Analysis of Plant Monosaccharide Transporters Using a Simple Growth Complementation Assay in Yeast
  8. 4828
    “…Over the last decades, PCR and molecular cloning have profoundly impacted various biological areas, from basic to pharmaceutical sciences. …”
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    A strategy to recover a poor-quality ligase product
  9. 4829
    Publicado 1993
    Tabla de Contenidos: “…Harrington and Noel J. Buckley -- Cloning of G protein-coupled receptors / Carola Eva and Rolf Sprengel -- Molecular cloning and expression of a Db1s dopamine receptor / Frederick J. …”
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    Receptors : molecular biology, receptor subclasses, localization, and ligand design
  10. 4830
    “…Somatic cell nuclear transfer (SCNT) has broad applications but is limited by low cloning efficiency. In this review, we mainly focus on SCNT-mediated epigenetic reprogramming in livestock and also describe mice data for reference. …”
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    Epigenetic Reprogramming During Somatic Cell Nuclear Transfer: Recent Progress and Future Directions
  11. 4831
    por Kent, Adrian
    Publicado 2019
    “…The impossibility of cloning devices can be derived from the impossibility of superluminal signalling and the projection postulate, together with assumptions about the devices’ location-independent functioning. …”
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    Summoning, No-Signalling and Relativistic Bit Commitments
  12. 4832
    por Hirota, Kiichi
    Publicado 2021
    “…In 2001, cDNA cloning of dioxygenases acting on prolines and asparagine residues, which play essential roles in this process, was reported. …”
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    HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
  13. 4833
    por Chen, Lishan, Oshima, Junko
    Publicado 2002
    “…Werner syndrome is a premature aging disease caused by the mutation in the WRN gene. The cloning and characterization of the WRN gene and its product allows investigators to study the disease and the human aging process at molecular level. …”
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    Werner Syndrome
  14. 4834
    “…SIMPLE SUMMARY: Different reproductive biotechnologies have been applied to sheep, such as cloning, which has been successfully applied in this species. …”
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    Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach
  15. 4835
    “…We compared our results to the national pool of physicians (N = 76,845) and medical specialists (N = 49,956) to identify cloning patterns based on Essed’s cultural cloning theory. …”
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    Diversity in the pathway from medical student to specialist in the Netherlands: a retrospective cohort study
  16. 4836
    “…However, many of the commercial vectors encoding different solubility tags require different cloning and purification steps for each vector, considerably slowing down expression screening. …”
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    A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production
  17. 4837
    “…CONCLUSIONS: We designed and tested a modular system to rapidly clone miRNA expression cassettes. Our strategy reduces the hands-on time required to successfully generate effective constructs, and can be implemented in labs with minimal molecular cloning expertise. …”
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    Construction of permanently inducible miRNA-based expression vectors using site-specific recombinases
  18. 4838
    “…Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich parasite DNA. …”
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    An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum
  19. 4839
    “…This mutant mdr1a cDNA may prove useful for efficient cloning of mdr1a in E. coli.…”
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    Identification of a Cryptic Bacterial Promoter in Mouse (mdr1a) P-Glycoprotein cDNA
  20. 4840
    “…The method explores NHEJ-cloning, genomic integration of a GFP-expressing plasmid without homologous arms and linearized in-cell. …”
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    A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid
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