Tabla de Contenidos: “…General methods -- Bacterial strains and cloning vectors -- Enzymes that modify DNA and RNA -- In vitro amplification of DNA using the polymerase chain reaction (PCR) and the thermostable Taq DNA polymerase -- DNA restriction fragment analysis and preparation -- In vitro labeling of probes and filter hybridization -- Plasmid DNA preparation from E coli hosts -- Preparation of DNA from lambda bacteriophage clones -- Subcloning fragments into plasmid vectors and plasmid construction -- Preparation of genomic DNA -- Preparation and analysis of RNA from eukaryotic cells -- Genomic cloning -- Preparation and analysis of cDNA libraries -- Preparation of subtractive cDNA -- Chain termination sequencing -- Transfection of mammalian cells -- Basic methods of protein analysis -- In situ hybridization -- Transgenic mouse preparation -- Detection and in vitro generation of specific mutations in genes and cDNAs -- Preparation of stock solutions -- Generally useful information -- Suppliers of reagents and equipment.…”