“…A stimulator of Fe transport (SFT) was identified by functional expression cloning in Xenopus oocytes. SFT-mediated transport has properties defined for transferrin-independent Fe uptake, but its cytolocalization in recycling endosomes and the observed stimulation of transferrin-bound Fe assimilation indicate a key role in intracellular Fe membrane transport as well. …”
Enlace del recurso
Enlace del recurso

“…To further understand the mechanisms of calcium regulation in signaling, we have cloned a Drosophila sodium/calcium-potassium exchanger, Nckx30C. …”
Enlace del recurso
Enlace del recurso

“…Continuous lines of murine cytotoxic T lymphocytes (CTL) directed to type A influenza viruses have been generated in vitro by stimulation of individual CTL precursors in the presence of T cell-growth factor TCGF and syngeneic virus-infected stimulator cells. The cloned CTL lines are H-2 restricted in their target cell recognition and exhibit distinct patterns of influenza virus recognition. …”
Enlace del recurso
Enlace del recurso

“…Previous studies have demonstrated that this clone binds antigen in an H-2-restricted manner. …”
Enlace del recurso
Enlace del recurso

“…Using antiidiotypic antibodies and T cell growth factor, P1- specific cytotoxic DA T cell clones were established by limiting dilution and kept in vitro. …”
Enlace del recurso
Enlace del recurso

“…The results of these studies prove unequivocally that cloned Lyt-2+ CTL can perform this task in an immunologically specific, H-2- restricted, and dose-dependent fashion.…”
Enlace del recurso
Enlace del recurso

“…Stimulation of a class II-restricted, antigen-specific T cell clone with interleukin 2 (IL-2) resulted in substantial increases in both cell surface IL-2 receptor (IL-2-R) and cytoplasmic IL-2-R messenger RNA (mRNA), whereas no increase was observed for cell-surface expression of Thy-1 and L3T4 antigens, and only a modest increase in Thy-1 mRNA was observed. …”
Enlace del recurso
Enlace del recurso

“…We have shown that cytotoxic T cell clones specific for the nominal antigen FL will bind high molecular weight (600,000 to 2,000,000) polyacrylamide and Ficoll polymers conjugated with 200-600 FL groups per molecule. …”
Enlace del recurso
Enlace del recurso

“…A panel of H-2k class II-restricted Th clones were established from individual CBA mice primed by infection with X31 influenza virus. 27 clones, which showed specific recognition of the HA surface glycoprotein, were all H3N2 subtype specific, in contrast to a T cell line which was crossreactive and which may have other specificities. 20 distinct HA-specific clones recognized a tryptic cleavage fragment of X31 consisting of residue 28-328 of HA1 (tops) which includes all the Ab-combining regions of the HA molecule. …”
Enlace del recurso
Enlace del recurso

Enlace del recurso
Enlace del recurso

“…At least some long-term in vitro-cultured cytotoxic T cell clones and uncloned cell populations are able, in the presence of Con A, to lyse other cells, to be lysed by other cells, but not to lyse themselves. …”
Enlace del recurso
Enlace del recurso

“…The CD3+, IL-2-dependent normal human thymocyte clone, CII, expresses on its surface a CD3-associated gamma/delta TCR. …”
Enlace del recurso
Enlace del recurso

“…By direct single cell cloning, we observed a number of long-term T cell clones that expressed CD45RA (2H4). …”
Enlace del recurso
Enlace del recurso

Enlace del recurso
Enlace del recurso

“…These results demonstrate a primary failure to censor forbidden clones of self-reactive T cells in inherited susceptibility to organ-specific autoimmune disease, and highlight the importance of thymic mechanisms of tolerance in organ-specific tolerance.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…We present a method, called fingerprint profiling (FPP), that uses restriction digest fingerprints of bacterial artificial chromosome clones to detect and classify rearrangements in the human genome. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…RESULTS: Here we present a hybrid cloning strategy which combines advantages of both recombinational and traditional cloning and which is particularly amenable to low-to-medium throughput investigations of protein function using techniques of molecular biochemistry and cell biology. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

Enlace del recurso
Enlace del recurso

“…From these, leukaemia clone-specific DNA primers were constructed and applied in nested polymerase chain reaction analyses of DNA extracted from the patients' Guthrie cards obtained at birth. …”
Enlace del recurso
Enlace del recurso
Enlace del recurso

“…It provides a first large scale cloning and characterization of wheat miRNAs and their predicted targets.…”
Enlace del recurso
Enlace del recurso
Enlace del recurso