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Biología molecular
5
Clonación molecular
3
Ingeniería genética
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Biología
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Bioquímica
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Biotecnología
2
Clonamiento molecular
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Fisiología
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Clone had no significant effect on specify gravity or moisture content
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Cromosomas
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Cuentos argentinos
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Cultivo de células
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Determinación del sexo
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El análisis de ranking evidenció cuatro clones superiores con potencial de mejoramiento
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El clon no tuvo efecto significativo sobre el peso específico ni el contenido de humedad
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Estética
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Genes
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541por Yan, Liu, Zhi-Qi, Meng, Bao-Long, Niu, Li-Hua, He, Hong-Biao, Weng, Wei-Feng, Shen“…(Lepidoptera: Bombycidae) gene encoding tryptophan oxygenase has been molecularly cloned and analyzed. The tryptophan oxygenase cDNA had 1374 nucleotides that encoded a 401 amino acid protein with an estimated molecular mass of 46.47 kDa and a PI of 5.88. …”
Publicado 2008
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542por Himeda, Toshiki, Hosomi, Takushi, Asif, Naeem, Shimizu, Hiroyuki, Okuwa, Takako, Muraki, Yasushi, Ohara, Yoshiro“…In order to encourage the molecular pathogenetic studies of SAFV, we generated an infectious cDNA clone of SAFV type 3 (SAFV-3). The present study demonstrated that the synthesis of the full-length infectious RNA by T7 RNA polymerase was terminated by a homologous sequence motif with the human preproparathyroid hormone (PTH) signal in the SAFV-3 genome. …”
Publicado 2011
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543por Farajnia, Safar, Rahbarnia, Leila, Maleki zanjani, Bahram, Alimohammadian, Mohammad Hossein, Abdoli Oskoee, Shahin, Beh-pajooh, Abbas, Saeedi, Nazli, Montazer Saheb, Soheila“…The aim of this study was to clone and characterize the gene encoding class I nuclease in L. infantum. …”
Publicado 2011
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544“…Their DNA binding domain consists of tandem arranged repeats, and due to this repetitive structure it is challenging to generate designer TALEs (dTALEs) with user-defined specificity. We present a cloning approach that facilitates the assembly of multiple repeat-encoding DNA fragments that translate into dTALEs with pre-defined DNA binding specificity. …”
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545por Sadeghi, H. Mir Mohammad, Ahmadi, R., Aghaabdollahian, S., Mofid, M.R., Ghaemi, Y., Abedi, D.“…The amplified product was entered into pTZ57R cloning vector by T/A cloning method and transformation was performed by heat shocking of the E. coli XL1-blue competent cells. …”
Publicado 2011
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546“…Among the fast sequencing systems, 454 liquid phase pyrosequencing provides the longest reads and is the most promising method for BAC clones. Here we report the results of pooled sequencing of BAC clones selected with ESTs genetically mapped to chromosome 3H. …”
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547por Cui, Xiaohong, Adler, Stuart P., Davison, Andrew J., Smith, Larry, Habib, EL-Sayed E., McVoy, Michael A.“…Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. …”
Publicado 2012
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551por Tijet, Nathalie, Tang, Patrick, Romilowych, Mya, Duncan, Carla, Ng, Victoria, Fisman, David N., Jamieson, Frances, Low, Donald E., Guyard, Cyril“…In the past 15 years, the incidence of some lineages distributed worldwide has tended to decrease, and local endemic clones and lineages have emerged. Comparative geographic distribution analysis suggests that some lineages are specific to eastern North America. …”
Publicado 2010
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552por Poulsen, Louise Ladefoged, Bisgaard, Magne, Son, Nguyen Thai, Trung, Nguyen Vu, An, Hoang Manh, Dalsgaard, AndersEnlace del recurso
Publicado 2012
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553por Mamedov, Ilgar Z, Britanova, Olga V, Bolotin, Dmitriy A, Chkalina, Anna V, Staroverov, Dmitriy B, Zvyagin, Ivan V, Kotlobay, Alexey A, Turchaninova, Maria A, Fedorenko, Denis A, Novik, Andrew A, Sharonov, George V, Lukyanov, Sergey, Chudakov, Dmitriy M, Lebedev, Yuri B“…Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.…”
Publicado 2011
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555por Chukeatirote, Ekachai, Maharachchikumbura, Sajeewa S. N., Wongkham, Shannaphimon, Sysouphanthong, Phongeun, Phookamsak, Rungtiwa, Hyde, Kevin D.“…The cbhI genes were then amplified and cloned using the pGEM-T Easy Vector Systems. The sizes of these PCR amplicons were between 700~800 bp. …”
Publicado 2012
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556por Baldan, Rossella, Testa, Francesca, Lorè, Nicola I., Bragonzi, Alessandra, Cichero, Paola, Ossi, Cristina, Biancardi, Anna, Nizzero, Paola, Moro, Matteo, Cirillo, Daniela M.“…Between 2006 and 2010, we observed the establishment of the ST22-IV clone displacing the predominant Italian clone, ST228-I, in a large Italian university hospital. …”
Publicado 2012
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557por Chen, Rong, Chang, Guobin, Zhang, Ying, Dai, Aiqin, Ma, Teng, Li, Jianchao, Zhai, Fei, Hua, Dengke, Xia, Mingxiu, Chen, Guohong“…In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the sequences of the PIWIL1 protein. …”
Publicado 2012
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558“…Although stem cell activity is thought to reside in the population of A(single) spermatogonia, new research suggests that clone size alone does not define the stem cell pool. …”
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559“…As a first step towards characterizing the innate immune response of MUC2 in different species, we report the cloning of a full-length, 11,359 bp chicken MUC2 cDNA, and describe the genomic organization and functional annotation of this complex, 74.5 kb locus. …”
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560por Zhang, Peng, Liu, Peng, Dou, Hongwei, Chen, Lei, Chen, Longxin, Lin, Lin, Tan, Pingping, Vajta, Gabor, Gao, Jianfeng, Du, Yutao, Ma, Runlin Z.“…In this study, we used the modified handmade cloning (HMC) established in cattle and pig to produce transgenic sheep with elevated levels of omega-3 (n−3) fatty acids. …”
Publicado 2013
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