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Mostrando 661 - 680 Resultados de 39,348 Para Buscar '"cloning"', tiempo de consulta: 0.29s Limitar resultados
  1. 661
    “…This aim helped in identifying superior clones for downstream breeding. In total, 220 cassava clones, three in uniform yield trials (UYTs) and 217 in a crossing block trial (CBT), were evaluated for virus and disease resistance. …”
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    Resistance of advanced cassava breeding clones to infection by major viruses in Uganda
  2. 662
    por Singh, Amar M.
    Publicado 2019
    “…In this protocol, I provide a method for utilizing FACS to generate single-cell clones of hPSCs with efficiencies approaching 40% within 7–10 days. …”
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    An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells
  3. 663
    “…DNA cloning remains the primary step before the further investigation of gene function. …”
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    ABC cloning: An efficient, simple, and rapid restriction/ligase-free method
  4. 664
    “…Amp-Seq based genotyping of longitudinal samples improves detection of minority clones and estimates of MOI. Amp-Seq permits tracking of clone density over time to study clone competition or the dynamics of specific, i.e. resistance-associated genotypes.…”
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    Longitudinal tracking and quantification of individual Plasmodium falciparum clones in complex infections
  5. 665
    “…At least two Potyviridae species cause the disease: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Cloned viral genome sequences known as infectious clones (ICs) have been important in the study of other viruses, both as a means of standardising infectious material and characterising viral gene function. …”
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    Strategies for the Construction of Cassava Brown Streak Disease Viral Infectious Clones
  6. 666
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    The development of a novel SNP genotyping assay to differentiate cacao clones
  7. 667
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    Differential expression, molecular cloning, and characterization of porcine beta defensin 114
  8. 668
    “…BACKGROUND: Long Adapter Single-Stranded Oligonucleotide (LASSO) probes were developed as a novel tool for massively parallel cloning of kilobase-long genomic DNA sequences. LASSO dramatically improves the capture length limit of current DNA padlock probe technology from approximately 150 bps to several kbps. …”
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    Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning
  9. 669
    “…DNA synthesis and homologous recombination can be used to simplify molecular cloning and to make synthetic biology easily accessible (M.J. …”
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    OVERFRAG: An overlapping DNA fragments generator for molecular cloning and synthetic biology
  10. 670
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    Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius
  11. 671
    “…However, there is no report on the construction of CA10 infectious clones. METHODS: The whole genome of CA10 derived from a clinical isolate was amplified into two fragments and ligated into a linearized plasmid vector in one step by In-Fusion Cloning. …”
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    Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
  12. 672
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    Molecular cloning and functional characterization of chalcone isomerase from Carthamus tinctorius
  13. 673
    “…Clones of both genotypes caused the maximum score of virulence at day 3 post-inoculation in LMH cells, although significant higher cytopathogenic score was found in ITS-OBT-Tg-1 genotype clones at days 1 and 2, as compared to clones with ITS-OBT-Tg-2. …”
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    Membrane associated proteins of two Trichomonas gallinae clones vary with the virulence
  14. 674
    “…Studies on genes and enzymes for biodegrading NOD can reveal the function and the biodegradation pathway of NOD. Based on successful cloning and expression of the USTB-05-A and USTB-05-C genes from Sphingopyxis sp. …”
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    Cloning and Expression of Genes for Biodegrading Nodularin by Sphingopyxis sp. USTB-05
  15. 675
    “…Modern cloning solutions are gradually replacing classical cloning methods. …”
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    MoCloFlex: A Modular Yet Flexible Cloning System
  16. 676
    “…Since its discovery, the stepwise characterization and cloning of RB1 have laid the foundation for numerous advances in the understanding of tumor suppressor genes, retinoblastoma tumorigenesis, and inheritance. …”
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    The RB1 Story: Characterization and Cloning of the First Tumor Suppressor Gene
  17. 677
    “…In this work we characterize the isolates and reconstruct the spread of a multi-clone epidemic event that occurred in an Intensive Care Unit in a hospital in Northern Italy. …”
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    Multiple Klebsiella pneumoniae KPC Clones Contribute to an Extended Hospital Outbreak
  18. 678
    “…PRNP gene encodes PrP protein, which is conservative among different species and associates with the susceptibility of prion disease. In this report, we cloned and sequenced the full-length PRNP gene of Vulpes corsac in Qinghai plateau, China. …”
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    Cloning and analysis of PRNP gene of Vulpes corsac in Qinghai plateau, China
  19. 679
    “…The amplified PCR products of TGEV HB06 were cloned, sequenced, and compared with other TGEV strains genes selected from the GenBank. …”
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    Cloning and expression of the membrane protein gene of TGEV HB06 strain
  20. 680
    “…N gene was cloned into BamHI and EcoRI multiple cloning sites of the prokaryotic expression vector pET 20 b, and named pETN. …”
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    Cloning and expression of nucleocapsid protein gene of TGEV HB06 strain
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