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“…One of the forms of the application of animal cloning is in agriculture. From an Islamic perspective, determining the permissibility of consuming cloned animals as food is essentially based on whether the cloned animal conforms to Islamic law’s principles and criteria. …”
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“…In this study, the cDNA sequence containing the complete coding frame of the costimulatory molecule duck CD40 gene was cloned and reported for the first time, and its mediated antiviral innate immune was verified in vitro. …”
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“…In the mid- to late 1970s, recombinant deoxyribonucleic acid methods for cloning and expressing genes in E. coli were under intense development. …”
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“…SIMPLE SUMMARY: The iron uptake systems are associated with virulence in colistin-resistant high-risk clones of K. pneumoniae. The isolates belonging to high-risk clones of K. pneumoniae are resistant to phagocytosis by neutrophils and induce NET formation. …”
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“…It is critical to curate routinely the epidemic P. aeruginosa clones encountered in the clinic. The aim of the present study was to investigate the connection between virulence factors and antimicrobial resistance profiles in epidemic clones. …”
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“…However, the conventional map-based cloning method is lengthy, requires a large mapping population and confirmation of many candidate genes in a broad genetic region to clone the causal variant. …”
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“…The present study sought to identify the promoter of the Ttyh1 gene and functionally characterize cis-regulatory elements that are potentially involved in the transcriptional regulation of Ttyh1 expression in rat dissociated hippocampal neurons in vitro. We cloned a 592 bp rat Ttyh1 promoter sequence and designed deletion constructs of the transcription factors specificity protein 1 (Sp1), E2F transcription factor 3 (E2f3), and achaete-scute homolog 1 (Ascl1) that were fused upstream of a luciferase reporter gene in pGL4.10[luc2]. …”
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“…The future direction into novel, universal, and high-efficiency methods for cloning NP BGCs is also prospected.…”
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“…In this study, we described a novel mechanism, seed cloning strategy, in a tropical plant species in countering animal predation. …”
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“…Gateway cloning employs the use of the ccdb toxin and has low colony numbers, making it difficult to apply at scale to clone libraries of cDNA vectors. …”
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“…OBJECTIVE: The clone devaluation is a phenomenon reported by the latest paper in which eeriness is evoked when people observe individuals with the same face (clone faces) compared to those with different faces. …”
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“…The use of homologous recombination clones, of PCR products as templates for T7 transcription, and of lipofection for large RNA transfection could increase the efficiency of infectious clone construction. …”
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“…BACKGROUND: Malaria parasites reproduce asexually, leading to the production of large numbers of genetically identical parasites, here termed a clonal line or clone. Infected hosts may harbor one or more clones, and the number of clones in a host is termed multiplicity of infection (MOI). …”
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“…A predicted metalloproteinase gene, HypZn, was cloned from Aspergillus niger CGMCC 3.7193 and expressed in Pichia pastoris GS115, and the physicochemical characteristics of recombinant HypZn were investigated after separation and purification. …”
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“…The transcriptomes of Pseudomonas aeruginosa clone C isolates NN2 and SG17M during the mid-exponential and early stationary phases of planktonic growth were evaluated by direct RNA sequencing on the nanopore platform and compared with established short-read cDNA sequencing on the Illumina platform. …”
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“…Here, via pump laser conveying and two-point locking, we implement re-generation of a Kerr soliton microcomb that faithfully clones the frequency and phase of another microcomb sent from 50 km away. …”
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“…RESULTS: The cDNA coding for the rat PAR4 homologue was cloned from the duodenum. Northern blots demonstrated a 3.0 kb transcript in the duodenum. …”
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“…Five truncated fragments of the LGALS12 promoter were cloned and the 4 kb fragment (L-4 kb) exhibited a high level of promoter activity in adipocytes and no promoter activity in non-adipocytes. …”
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“…Here, we describe a high-throughput protocol for cloning, expressing, purifying, and evaluating bispecific antibodies. …”
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